Laser-triggered collaborative chemophotothermal effect of gold nanoparticles for targeted colon cancer therapy

Zhang, Yajie; Zhou, Lu; Tan, Jingwei; Liu, Jianling; Shan, Xiaoqing; Ma, Yong

doi:10.1016/j.biopha.2020.110492

Cited by 18 publications

(16 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This investigation suggested that nucleolin played an important role in the acceleration of CRC growth via a signaling of phosphatase of regenerating liver-3. In addition, the binding interaction between ASA and nucleolin enhanced the specificity of gold nanoparticles to CRC cells which was beneficial in chemophotothermal therapy (Zhang et al, 2020b).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, cytotoxicity level of Dox reported in this work was in the same order of magnitude as the results from other research groups. For instance, after treating the cells with Dox for 24, 48, and 72 hours, the IC 50 values were 65.25 µM (Xu et al, 2003), 0.35 µM (Zhang et al, 2020b) and 0.29 µM (Mielczarek-Puta et al, 2019), respectively. This might indicate that cytotoxicity of Dox increased with the longer treatment durations.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Anti-Proliferative Effect of Doxorubicin-Loaded AS1411 Aptamer on Colorectal Cancer Cell

Lohlamoh

Soontornworajit

Rotkrua

2021

Asian Pac J Cancer Prev

View full text Add to dashboard Cite

Background: Doxorubicin (Dox) inhibits DNA replication and causes DNA damage resulting in cell death. It is a common drug for treatment of many cancers. Treatment efficacy and side effects of Dox are critical issues in using it because the drug lacks of specificity. The objective of this study was to improve the specificity of Dox by the incorporation of this drug with AS1411 aptamer (ASA). Methods: Dox was intercalated into the duplex sites of ASA, a recognition molecule for a number of cancer cells, and formed Dox-loaded ASA. The recognition ability proceeded through specific binding between the aptamer and nucleolin overexpressed in the cancer cells. The tested cells were human colorectal adenocarcinoma cell line (SW480) and human normal colon cell CCD841 CoN (CCD841). Binding of ASA to the cells was tested using flow cytometer and fluorescence microscope. Intercalation of Dox into DNA duplex was confirmed by fluorescence spectrometry. Effect of ASA, Dox, and Dox-loaded ASA on cell viability was examined by cell proliferation assay. Caspase-3 activation was analyzed by western blotting. Results: ASA bound specifically to SW480 cells via interaction between the aptamer and nucleolin because the nucleolin was highly expressed in SW480 cells. ASA decreased the viability of SW480 cells in a dose-dependent manner. Dox was more toxic than ASA. Fluorescence quenching revealed that Dox was able to intercalate in base pairing sites of the aptamer. Dox-loaded ASA inhibited the proliferation of SW480 cells, because the aptamer facilitated the Dox uptake into these cells which caused the cell apoptosis, indicated by the significant decrease in procaspase-3, apoptosis marker protein. Conclusion:This study succeeded to prepare Dox-loaded ASA by intercalation of the drug that inherited the binding function from the aptamer and anti-cancer activity from Dox. Dox-loaded ASA showed promise for effective cancer treatment with lower level of side effects.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Anti-Proliferative Effect of Doxorubicin-Loaded AS1411 Aptamer on Colorectal Cancer Cell

Lohlamoh

Soontornworajit

Rotkrua

2021

Asian Pac J Cancer Prev

View full text Add to dashboard Cite

show abstract

“…Among them, combined chemotherapy and photothermal treatment has e cient antitumor effects. For example, Y. Zhang et al synthesized laser-triggered gold nanoparticles with synergistic chemotherapyphotothermal effect for targeted colon cancer therapy [30]. Rong Ma et al constructed a therapeutic indocyanine green-based nanoplatform for near-infrared uorescent image-guided chemotherapy/ photothermal treatment of cervical cancer [31].…”

Section: Discussionmentioning

confidence: 99%

AS1411-targeted graphene oxide nano-drug delivery system for chemo-photothermal therapy of cervical cancer

Gao

Liang

Che

et al. 2022

Preprint

View full text Add to dashboard Cite

The aim of this study is to design a novel pH and photothermal dual-responsive nanodrug delivery system with high biocompatibility and tumor targeting. Based on the high loading rate and good photothermal properties of graphene oxide (GO), we selected GO modified with chitosan (CO) and γ-polyglutamic acid (γ-PGA) as nanocarriers. CO and γ-PGA increased the dispersion of GO and improved the solubility of GO-CO-γ-PGA (G-C-P) in solution. To further improve the targeting of the system, the nucleic acid aptamer NH2-AS1411 (APT), which targets the nucleolin (C23), was attached to G-C-P and the targeted nano-delivery system APT-GO-CO-γ-PGA (A-G-C-P) was prepared by amidation. The synthesized samples were characterized using Fourier transform infrared spectroscopy (FTIR), Ultraviolet-visible spectrophotometer (UV-Vis), X-ray diffraction (XRD), Transmission electron microscopy (TEM), Dynamic light scattering (DLS) and Zeta potential, and their good biocompatibility and stable photothermal conversion properties were demonstrated experimentally. The nanocarrier can act as a photothermal agent, generating high temperatures to induce cell damage. The lower hemolysis rate reflects the good biocompatibility of the carrier. Doxorubicin hydrochloride (DOX) was selected as the model drug with a nanocarrier loading of 37.0 ± 0.74%, showing dual response drug release characteristics of pH and NIR light. The cytotoxicity and photothermal toxicity of the nanocarrier A-G-C-P and the drug delivery system APT-GO-CO-γ-PGA-DOX (A-G-C-P-D) on HeLa cells were investigated in cellular experiments. It was found that A-G-C-P had almost no toxic side effects on the cells, and the killing effect of A-G-C-P-D with the application of near-infrared light irradiation was more obvious, proving its good photothermal therapeutic effect, and further demonstrating that the combined effect of chemotherapy and photothermal treatment is significant than that of single treatment. Confocal microscopy and flow cytometry determined the distribution of DOX in the cells to different degrees after cell internalization. In vivo anti-tumor experiments were conducted to further investigate the therapeutic effects and safety of the targeted nano-drug delivery system in concert with photothermal. Compared with the weight loss and heart tissue damage caused by free DOX, A-G-C-P-D and NIR light irradiation did not cause any tissue damage and toxic side effects in nude mice, and have good biosafety; A-G-C-P-D and NIR light irradiation can inhibit tumor growth, cause damage to tumor tissue, significantly reduce Ki67 expression and increase Caspase-3 expression, all of which prove their good anti-tumor effects. This relatively non-invasive approach may provide a good direction for targeted drug delivery and chemotherapeutic photothermal treatment of tumors, greatly reducing the side effects of chemotherapy. The therapeutic effect of this nano-drug delivery system provides new ideas for clinical treatment strategies with potential applications and reference values, offering a broad prospect for biological applications.

show abstract

“…And some researches indicate that the combination use of 5-FU, levamisole, and leucovorin in stages II and III colon cancer patients show survival benefit ( 39 ). Other researchers also find combination therapy is effective for treating colon cancer in vitro and in vivo ( 40 , 41 ). But effective combination treatment by novel targeting drugs with lower toxicity is still in exploration.…”

Section: Discussionmentioning

confidence: 99%

Combination of Auranofin and ICG-001 Suppress the Proliferation and Metastasis of Colon Cancer

Lin

Zhao

et al. 2021

Front. Oncol.

View full text Add to dashboard Cite

Colon cancer is one of the deadliest tumors in the world, and with high metastasis rate and mortality, effective drugs for its treatment are still in need. Auranofin (AF) is a gold complex that has been attested by FDA for treating human rheumatism, and researchers have found that AF acts as a great antitumor drug in recent years. ICG-001 is a small molecule inhibitor of Wnt/β-catenin pathway. In the present study, we aimed to explore the synergistic antitumor effects and the underlying mechanisms of AF and ICG-001 combination therapy on human colon cancer. The results showed that AF and ICG-001 synergistically depressed the growth and invasion of human colon cancer cells by inhibiting the phosphorylation of Signal Transducer and Activator of Transcription 3 (STAT3) and its downstream mediator B-cell lymphoma-2-like 1 (Bcl-xL) and inducing caspase-3-dependent apoptosis. Moreover, AF combined with ICG-001 synergistically inhibited the growth of colon cancer in subcutaneous xenograft mice models and restrained metastasis in lung metastasis mice models. In conclusion, our results demonstrated that combination of AF and ICG-001 suppressed the proliferation and metastasis of colon cancer by inhibiting STAT3 phosphorylation. Therefore, this combination therapy may possess potential therapeutic properties for human colon cancer.

show abstract

Laser-triggered collaborative chemophotothermal effect of gold nanoparticles for targeted colon cancer therapy

Cited by 18 publications

References 41 publications

Anti-Proliferative Effect of Doxorubicin-Loaded AS1411 Aptamer on Colorectal Cancer Cell

Anti-Proliferative Effect of Doxorubicin-Loaded AS1411 Aptamer on Colorectal Cancer Cell

AS1411-targeted graphene oxide nano-drug delivery system for chemo-photothermal therapy of cervical cancer

Combination of Auranofin and ICG-001 Suppress the Proliferation and Metastasis of Colon Cancer

Contact Info

Product

Resources

About