Laser-treated electrospun fibers loaded with nano-hydroxyapatite for bone tissue engineering

Abstract

Core-shell polycaprolactone/polycaprolactone (PCL/PCL) and polycaprolactone/polyvinyl acetate (PCL/PVAc) electrospun fibers loaded with synthesized nanohydroxyapatite (HAn) were lased treated to create microporosity. The prepared materials were characterized by XRD, FTIR, TEM and SEM. Uniform and randomly oriented beadless fibrous structures were obtained in all cases. Fibers diameters were in the 150-300nm range. Needle-like HAn nanoparticles with mean diameters of 20nm and length of approximately 150nm were mostly encase inside the fibers. Laser treated materials present micropores with diameters in the range 70-120μm for PCL-HAn/PCL fibers and in the 50-90μm range for PCL-HAn/PVAC material. Only samples containing HAn presented bioactivity after incubation during 30days in simulated body fluid. All scaffolds presented high viability, very low mortality, and human osteoblast proliferation. Biocompatibility was increased by laser treatment due to the surface and porosity modification.

Composite scaffold obtained by electro-hydrodynamic technique for infection prevention and treatment in bone repair

“…Specially, synthetic polymers, such as polylactic acid (PLA), polyglycolic acid (PGA), poly(lactic-coglycolic acid) (PLGA), or polycaprolactone (PCL), facilitate control of the physicochemical and mechanical features of the synthesised scaffolds (Dorati et al, 2017;Ghassemi et al, 2018;Liu and Ma, 2004). In particular, electrospun PCL fibres have been previously reported as highly mimetic of the extracellular matrix and promising for bone regeneration strategies, combining the versatility and simplicity of the electrospinning technique with the biocompatibility of PCL and its degradation products (Aragon et al, 2017;Baker et al, 2016;Porter et al, 2009;Song et al, 2013).…”

Covalent immobilization of acetylcholinesterase on a novel polyacrylic acid‐based nanofiber membrane

“…Electrospinning is a suitable and versatile technique for producing NFs from a blend of chemical solution with NF distances across going from several micrometers down to various nanometers. Therefore, the NF membranes are preferable candidates for numerous areas such as, pharmaceutical industry, tissue engineering, cosmetics, clothing, electric devices, and enzyme immobilization . Poly(acrylic acid) (PAA) is an important industrial polymer with its low toxicity, which is generally used as a superabsorbent polymer, a scale inhibitor, and dispersing agent .…”

Production, characterization and testing of antibacterial PVA membranes loaded with HA‐Ag3PO4 nanoparticles, produced by SC‐CO2 phase inversion

“…(b)] confirmed their morphology and structure. The needle‐like shape of the nanoparticles was due to a preferred growth orientation along the c‐axis . SEM image in Fig.…”