Laser technique for the fabrication of blood vessels-like models for preclinical studies of pathologies under flow conditions

Abstract

In this work a method for fabricating functionalized preclinical devices is presented. The manufacturing process combines a laser indirect writing technique to fabricate a soda-lime glass master and soft-lithography methods to obtain the final structure in polydimethylsiloxane (PDMS). The roughness of the device is modified in a controlled manner by applying a post-thermal treatment to the master, and thus devices with different roughness values are created. The PDMS devices are fully covered with human umbilical vein cells in a two-step process. In order to determine the most suitable device to perform bioassays, the cell attachment to the channel is evaluated with regards to the walls roughness when flow experiments are carried out.

Determination of hemodynamic risk for vascular disease in planar artery bifurcations

“…However, because the channel through which the flow passes is embedded in a PDMS structure that limits the movements of the walls and taking into account the range of pressures that are reached inside the channel, it seems appropriate to assume the consideration of rigid walls (condition also used in numerical simulations). The fabrication of the device that was used in the experimental validation of the simulations is described in 24 . Briefly, laser technologies were used for manufacturing the channel over soda-lime glass.…”

Synergism between obesity and HFpEF on neutrophils phenotype and its regulation by adipose tissue‐molecules and SGLT2i dapagliflozin

“…The fluidic device was a concave Y‐shaped channel made from polydimethylsiloxane (PDMS, Sylgard 184 from Dow Corning) following the method previously described. 32 For the experiments, HUVEC at confluence were detached with trypsin (0.25% in Hank's balanced salt solution with 1 mM EDTA (Thermo Fisher Scientific, Waltham, MA, USA) and seeded in fibronectin (5 μg/ml in 0.02% gelatine; Gibco®) the pre‐coated PDMS channels at a concentration of 1.5 × 10 6 cells/ml and maintained overnight until the start of the fluidic experiment. Perfusion started at 0.5 ml/min with EGM‐2 medium to avoid damage to the monolayer, velocity was doubled every hour until reach a flow‐rate of 6 ml/min, that was maintained for 4 h. Differentiated HL‐60 with or without CD11b upregulation were labelled with calcein‐AM (Invitrogen®, Thermo Fisher Scientific, Waltham, MA, USA) at a 1 μM concentration for 20 min.…”

“…The fluidic device was a concave Y‐shaped channel made from polydimethylsiloxane (PDMS, Sylgard 184 from Dow Corning) following the method previously described 32 . For the experiments, HUVEC at confluence were detached with trypsin (0.25% in Hank's balanced salt solution with 1 mM EDTA (Thermo Fisher Scientific, Waltham, MA, USA) and seeded in fibronectin (5 μg/ml in 0.02% gelatine; Gibco®) the pre‐coated PDMS channels at a concentration of 1.5 × 10 6 cells/ml and maintained overnight until the start of the fluidic experiment.…”

Internal Microchannel Manufacturing Using Stereolithographic 3D Printing

“…In addition to its multiple applications in chemistry, engineering or sensing, microfluidics is of great interest in medicine and pharmacology, where one of the challenges is to manufacture complex devices capable of mimicking physiological structures [9][10][11], such as vessels, veins and arteries, where novel drugs can be tested under static and flow conditions (dynamic regime). These studies, much closer to reality than the studies carried out by traditional methods, involving testing in wells (static regime), could decrease the animal experimentation needed for testing drugs before the patient dispensation.…”