Laser-targeted ablation of the zebrafish embryonic ventricle: A novel model of cardiac injury and repair

Matrone, Gianfranco; Taylor, Jonathan; Wilson, Kevin S.; Baily, James; Love, Gordon D.; Girkin, John M.; Mullins, John J.; Tucker, Carl; Denvir, Martin A.

doi:10.1016/j.ijcard.2013.06.063

Cited by 41 publications

(51 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Zebrafish have been used successfully as a model for different types of wounds including laser injury [13][14][15] , laser-induced thrombosis 16 , and epithelial wounding 10 . We report a method of mechanical wounding that is simple to execute and produces a controlled injury in an in vivo model that is highly amenable to real-time microscopy.…”

Section: Discussionmentioning

confidence: 99%

“…Injury to the embryo results in a highly conserved cellular response from cells at the injury site and the innate immune cells recruited to respond to the injury and regulate its resolution 11,12 . Other injury models have used focused laser pulses to spatially localize injury to specific cell types including neurons, muscle cells, and cardiomyocytes [13][14][15] . Zebrafish embryos have been used as a model to study hemostasis and thrombosis in conditions of pharmacological and genetic manipulation, using both mechanical and laser-induced thrombus formation [16][17][18][19] .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mechanical Vessel Injury in Zebrafish Embryos

Clay¹,

Coughlin²

2015

JoVE

View full text Add to dashboard Cite

Zebrafish (Danio rerio) embryos have proven to be a powerful model for studying a variety of developmental and disease processes. External development and optical transparency make these embryos especially amenable to microscopy, and numerous transgenic lines that label specific cell types with fluorescent proteins are available, making the zebrafish embryo an ideal system for visualizing the interaction of vascular, hematopoietic, and other cell types during injury and repair in vivo. Forward and reverse genetics in zebrafish are well developed, and pharmacological manipulation is possible. We describe a mechanical vascular injury model using micromanipulation techniques that exploits several of these features to study responses to vascular injury including hemostasis and blood vessel repair. Using a combination of video and timelapse microscopy, we demonstrate that this method of vascular injury results in measurable and reproducible responses during hemostasis and wound repair. This method provides a system for studying vascular injury and repair in detail in a whole animal model. Video LinkThe video component of this article can be found at

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mechanical Vessel Injury in Zebrafish Embryos

Clay¹,

Coughlin²

2015

JoVE

View full text Add to dashboard Cite

show abstract

“…Means±s.e.m. are shown in each figure. ( Matrone et al, 2013). This functional loss recovers fully within 24 h. Inhibition of Cdk9 activity, with either flavopiridol or morpholinos, resulted in a reduced capacity of the ventricle to recover from injury.…”

Section: Response To Laser Injurymentioning

confidence: 94%

“…Laser injury of the zebrafish embryonic heart was induced using a XYclone laser (Hamilton-Thorne, USA), using a technique that has been previously reported by our group (Matrone et al, 2013). The protocol for laser injury and recovery is summarised in Fig.…”

Section: Heart Laser Injurymentioning

confidence: 99%

CDK9 and its repressor LARP7 modulate cardiomyocyte proliferation and response to injury in the zebrafish heart

Matrone¹,

Wilson²,

Maqsood³

et al. 2016

Development

Self Cite

View full text Add to dashboard Cite

Cyclin dependent kinase (Cdk)9 acts through the positive transcription elongation factor-b (P-TEFb) complex to activate and expand transcription through RNA polymerase II. It has also been shown to regulate cardiomyocyte hypertrophy, with recent evidence linking it to cardiomyocyte proliferation. We hypothesised that modification of CDK9 activity could both impair and enhance the cardiac response to injury by modifying cardiomyocyte proliferation. Cdk9 expression and activity were inhibited in the zebrafish (Danio rerio) embryo. We show that dephosphorylation of residue Ser2 on the C-terminal domain of RNA polymerase II is associated with impaired cardiac structure and function, and cardiomyocyte proliferation and also results in impaired functional recovery following cardiac laser injury. In contrast, de-repression of Cdk9 activity, through knockdown of La-related protein (Larp7) increases phosphorylation of Ser2 in RNA polymerase II and increases cardiomyocyte proliferation. Larp7 knockdown rescued the structural and functional phenotype associated with knockdown of Cdk9. The balance of Cdk9 and Larp7 plays a key role in cardiomyocyte proliferation and response to injury. Larp7 represents a potentially novel therapeutic target to promote cardiomyocyte proliferation and recovery from injury.

show abstract

“…For example, in liver regeneration, larger damage of the adult liver lobes usually makes fish die quickly. Other physical injuries are also applied, for example, laser targeted cell ablation is mainly used to induce lesions of retina photoreceptor and heart muscles recently [8,15]. Chemical injury is based on the toxicity of the compounds to the target organs.…”

Section: Organ Injury Approaches Applied In Zebrafishmentioning

confidence: 99%

Using zebrafish as the model organism to understand organ regeneration

Shi

Fang

et al. 2015

Sci. China Life Sci.

View full text Add to dashboard Cite

The limited regenerative capacity of several organs, such as central nervous system (CNS), heart and limb in mammals makes related major diseases quite difficult to recover. Therefore, dissection of the cellular and molecular mechanisms underlying organ regeneration is of great scientific and clinical interests. Tremendous progression has already been made after extensive investigations using several model organisms for decades. Unfortunately, distance to the final achievement of the goal still remains. Recently, zebrafish became a popular model organism for the deep understanding of regeneration based on its powerful regenerative capacity, in particular the organs that are limitedly regenerated in mammals. Additionally, zebrafish are endowed with other advantages good for the study of organ regeneration. This review summarizes the recent progress in the study of zebrafish organ regeneration, in particular regeneration of fin, heart, CNS, and liver as the representatives. We also discuss reasons of the reduced regenerative capacity in higher vertebrate, the roles of inflammation during regeneration, and the difference between organogenesis and regeneration. organ regeneration, zebrafish, fin, heart, liver, CNS Citation:Shi WC, Fang ZB, Li L, Luo LF. Using zebrafish as the model organism to understand organ regeneration.

show abstract

Laser-targeted ablation of the zebrafish embryonic ventricle: A novel model of cardiac injury and repair

Cited by 41 publications

References 27 publications

Mechanical Vessel Injury in Zebrafish Embryos

Mechanical Vessel Injury in Zebrafish Embryos

CDK9 and its repressor LARP7 modulate cardiomyocyte proliferation and response to injury in the zebrafish heart

Using zebrafish as the model organism to understand organ regeneration

Contact Info

Product

Resources

About