Laser Surface Microstructuring of a Bio-Resorbable Polymer to Anchor Stem Cells, Control Adipocyte Morphology, and Promote Osteogenesis

Ortiz, Rocío; Aurrekoetxea-Rodríguez, Iskander; Rommel, Mathias; Quintana, Iban; Vivanco, María dM; Toca-Herrera, José L.

doi:10.3390/polym10121337

Cited by 24 publications

(27 citation statements)

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“…Its main advantage over other treatments is that it modifies the surface selectively, without destroying the material or producing toxic substances. The studies published so far address the influence of various types of laser treatment (using CO 2 , picosecond, excimer lasers) on the surface of various polymers: polycaprolactone, [ 49 , 50 ] polymethylsiloxane [ 51 ], polyethylene terephthalate [ 52 , 53 ], poly-L-lactic-acid [ 54 , 55 ], poly(L-lactide-co-glycolide) [ 56 ], chitosan, and chitosan/ceramics composites [ 57 ].…”

Section: Introductionmentioning

confidence: 99%

“…Analysis of the most recent published studies, including a thorough review by Professor Ravi-Kumar et al [ 58 ], key experimental works [ 28 , 48 , 54 , 55 ], and the latest studies [ 56 , 57 , 58 , 59 , 61 , 62 ] has provided the basis for summarizing the currently available data and existing notions of the effects produced by laser treatment of polymer materials. Laser ablation is widely used today to treat various materials (metals, ceramics, glass, and polymers).…”

Section: Introductionmentioning

confidence: 99%

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Laser Processing of Polymer Films Fabricated from PHAs Differing in Their Monomer Composition

et al. 2021

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The study reports results of using a CO2-laser in continuous wave (3 W; 2 m/s) and quasi-pulsed (13.5 W; 1 m/s) modes to treat films prepared by solvent casting technique from four types of polyhydroxyalkanoates (PHAs), namely poly-3-hydroxybutyrate and three copolymers of 3-hydroxybutyrate: with 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate (each second monomer constituting about 30 mol.%). The PHAs differed in their thermal and molecular weight properties and degree of crystallinity. Pristine films differed in porosity, hydrophilicity, and roughness parameters. The two modes of laser treatment altered these parameters and biocompatibility in diverse ways. Films of P(3HB) had water contact angle and surface energy of 92° and 30.8 mN/m, respectively, and average roughness of 144 nm. The water contact angle of copolymer films decreased to 80–56° and surface energy and roughness increased to 41–57 mN/m and 172–290 nm, respectively. Treatment in either mode resulted in different modifications of the films, depending on their composition and irradiation mode. Laser-treated P(3HB) films exhibited a decrease in water contact angle, which was more considerable after the treatment in the quasi-pulsed mode. Roughness parameters were changed by the treatment in both modes. Continuous wave line-by-line irradiation caused formation of sintered grooves on the film surface, which exhibited some change in water contact angle (76–80°) and reduced roughness parameters (to 40–45 mN/m) for most films. Treatment in the quasi-pulsed raster mode resulted in the formation of pits with no pronounced sintered regions on the film surface, a more considerably decreased water contact angle (to 67–76°), and increased roughness of most specimens. Colorimetric assay for assessing cell metabolic activity (MTT) in NIH 3T3 mouse fibroblast culture showed that the number of fibroblasts on the films treated in the continuous wave mode was somewhat lower; treatment in quasi-pulsed radiation mode caused an increase in the number of viable cells by a factor of 1.26 to 1.76, depending on PHA composition. This is an important result, offering an opportunity of targeted surface modification of PHA products aimed at preventing or facilitating cell attachment.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Laser Processing of Polymer Films Fabricated from PHAs Differing in Their Monomer Composition

et al. 2021

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“…To obtain a better and faster healing, researchers have introduced the polymeric materials capable of offering support, but also of being seeded with stem cells (Liu et al, 2013). For osteoporosis, natural materials such as green tea can be used for differentiation and guidance of bone marrow thus decreasing the bone resorption (Ortiz et al, 2018). Laser surface micro structuring is a method that, according to some recent studies, can promote osteogenesis by modifying the surface topography of a biomaterial which includes stem cells.…”

Section: Hard Tissue Engineeringmentioning

confidence: 99%

“…Laser surface micro structuring is a method that, according to some recent studies, can promote osteogenesis by modifying the surface topography of a biomaterial which includes stem cells. Pores, grooves or pits can induce the transformation of mesenchymal stem cells into a tissue with specific properties (Béduer et al, 2012, Khang et al, 2012, Ortiz et al, 2018.…”

Section: Hard Tissue Engineeringmentioning

confidence: 99%

Strategies based on stem cells for tissue engineering

2018

Biomater Tissue Eng Bull

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Cell-therapy is a new approach in medical research field. The increased number of patients, which is reported all over the world, has led to the development of innovative solutions capable of improving wound healing. Stem cells, due to their self-renewal and differentiation capacities, gradually replace the traditional medicine used in the past. Even if the risks of using stem cells exists, the promising results they can offer have led to the design of intelligent materials that can make life easier for patients. Stem cells can be classified by many criteria, but the most important are embryonic and adult stem cells. The ability of embryonic stem cells of differentiate into any type of tissue by various strategies makes them an ideal candidate in tissue engineering applications. Ethical problems must be taken into consideration, so the most used in different therapies are adult stem cells. Nowadays, the applications of stem cells are various. They can be used in order to restore the function of soft and hard tissue and to improve the healing time of a wound. Skin problems, osteogenesis, angiogenesis or fracture healing are some examples of tissue disorders that can be restored by using stem cells therapy and nanotechnology.

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“…Laser ablation by CO2 and short-pulsed (nanoseconds) lasers has been applied before on polyhydroxy butyrate (P3HB) to cut the material or modify its surface or bulk properties [14][15][16][17][18]. However, ultrashort pulsed lasers (in the picosecond and the femtosecond range) allow to generate surface microfeatures with higher precision and minimal thermal and chemical impact on biodegradable and biocompatible polymers [19,20]. Here, the response of novel blends of short-chain length (scl-) and medium-chain length (mcl-) PHAs to ps pulsed laser ablation at wavelengths of 355 and 532 nm is reported for the first time.…”

Section: Introductionmentioning

confidence: 99%

Picosecond Laser Ablation of Polyhydroxyalkanoates (PHAs): Comparative Study of Neat and Blended Material Response

Ortiz¹,

Basnett

Roy

et al. 2020

Polymers

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Polyhydroxyalkanoates (PHAs) have emerged as a promising biodegradable and biocompatible material for scaffold manufacturing in the tissue engineering field and food packaging. Surface modification is usually required to improve cell biocompatibility and/or reduce bacteria proliferation. Picosecond laser ablation was applied for surface micro structuring of short- and medium-chain length-PHAs and its blend. The response of each material as a function of laser energy and wavelength was analyzed. Picosecond pulsed laser modified the surface topography without affecting the material properties. UV wavelength irradiation showed halved ablation thresholds compared to visible (VIS) wavelength, revealing a greater photochemical nature of the ablation process at ultraviolet (UV) wavelength. Nevertheless, the ablation rate and, therefore, ablation efficiency did not show a clear dependence on beam wavelength. The different mechanical behavior of the considered PHAs did not lead to different ablation thresholds on each polymer at a constant wavelength, suggesting the interplay of the material mechanical parameters to equalize ablation thresholds. Blended-PHA showed a significant reduction in the ablation threshold under VIS irradiation respect to the neat PHAs. Picosecond ablation was proved to be a convenient technique for micro structuring of PHAs to generate surface microfeatures appropriate to influence cell behavior and improve the biocompatibility of scaffolds in tissue engineering.

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Laser Surface Microstructuring of a Bio-Resorbable Polymer to Anchor Stem Cells, Control Adipocyte Morphology, and Promote Osteogenesis

Cited by 24 publications

References 58 publications

Laser Processing of Polymer Films Fabricated from PHAs Differing in Their Monomer Composition

Laser Processing of Polymer Films Fabricated from PHAs Differing in Their Monomer Composition

Strategies based on stem cells for tissue engineering

Picosecond Laser Ablation of Polyhydroxyalkanoates (PHAs): Comparative Study of Neat and Blended Material Response

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