Laser speckle contrast imaging of blood microcirculation in pancreatic tissues during laparoscopic interventions

Abstract

Laser speckle contrast imaging of the microcirculatory bed of the pancreas is performed, which allows its condition to be assessed and thereby is an additional valuable tool for making a diagnostic decision and dynamically monitoring the effectiveness of the treatment for pathology of the abdominal organs. Laparoscopic operations on the pancreas are low-traumatic and in most cases avoid open surgical interventions. For the first time an experimental system for recording speckle images, combined with a commercially available five-millimetre rigid laparoscope, is presented. The sensitivity of the system to the fluid motion in a capillary at different velocities is determined, and the possibility of finding areas of blood microcirculation disturbance in modelling pancreatic ischemia in an experiment on laboratory animals is revealed. The laparoscope illumination channel is verified by comparison with speckle dynamics under external illumination of the studied object.

Laser speckle contrast imaging and machine learning in application to physiological fluids flow rate recognition

“…The proposed technic or any of its elements are applicable to the LSCI or LDF methods in the "in vivo" conditions. For example, when studying soft tissues microcirculation [5][6][7] and when the obtained data samples can be represented in form of one-or multi-dimensional sequences of 10 2 -10 3 elements. For the bigger samples, the deep neural networks should be met.…”

“…The study was implemented with the experimental setup that combines the fluid flow circuit and the LSCI equipment (see Fig. 1), designed earlier [6,8,9]…”

“…The study of the rheological and kinematic properties of physiological fluids is an actual task, and of particular interest are minimally invasive and non-invasive diagnostic methods. The laser Doppler flowmetry (LDF) and the laser speckle contrast imaging (LSCI) methods make it possible to carry out studies in the "in vivo" conditions to evaluate microcirculation in biological tissues [5][6][7]. LSCI is used in rheumatology, dermatology, ophthalmology, neurology, and many other areas of medicine [8][9][10].…”

Brain metabolism changes in cases of impaired breathing or blood circulation in rodents evaluated by real time optical spectroscopy methods

“…The setup for laser speckle contrast imaging consisted of a compact laser module LDM785 with a working wavelength of 785 nm and a power of 20 mW (Thorlabs, USA), a diffuser DG10-1500 (Thorlabs, USA), a polarizer LPNIRE100-B (Thorlabs, USA) and a holder for a polarizer RSP1/M (Thorlabs, USA), as well as a fixed focus lens MVL25TM23 (Thorlabs, USA). Blood flow dynamics was recorded with IDS-3260CP-M-GL R2 camera (IDS Imaging Development Systems GmbH, Germany) 10 .…”

Biophotonics methods for functional monitoring of complications of diabetes mellitus

“…These sites are most convenient for research as closely lying vessels provide a higher signal-to-noise ratio. Later, this technology was applied in surgical interventions [123,124].…”