Laser speckle contrast analysis (LASCA) technology for the semiquantitative measurement of angiogenesis in in-ovo-tumor-model

Pion, Eric; Asam, Claudia; Feder, Anna-Lena; Felthaus, Oliver; Heidekrueger, Paul I.; Prantl, Lukas; Aung, Tin

doi:10.1016/j.mvr.2020.104072

Cited by 20 publications

(20 citation statements)

References 48 publications

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“…The 3D in vivo tumour model (CAM, chorioallantoic membrane) was carried out as described in previous publications ( Feder et al, 2020 ; Pion et al, 2020 ; Troebs et al, 2020 ). Fertilized chicken eggs were incubated in a ProCon egg incubator (Grumbach) at 37.8°C and 63% humidity under hourly rotation for a 4-d period until a window of ∼1 × 1 cm was cut into the eggshell and sealed again with tape.…”

Section: Methodsmentioning

confidence: 99%

Cancer-associated cells release citrate to support tumour metastatic progression

Drexler

Schmidt²,

Jordan³

et al. 2021

Life Sci. Alliance

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Citrate is important for lipid synthesis and epigenetic regulation in addition to ATP production. We have previously reported that cancer cells import extracellular citrate via the pmCiC transporter to support their metabolism. Here, we show for the first time that citrate is supplied to cancer by cancer-associated stroma (CAS) and also that citrate synthesis and release is one of the latter’s major metabolic tasks. Citrate release from CAS is controlled by cancer cells through cross-cellular communication. The availability of citrate from CAS regulated the cytokine profile, metabolism and features of cellular invasion. Moreover, citrate released by CAS is involved in inducing cancer progression especially enhancing invasiveness and organ colonisation. In line with the in vitro observations, we show that depriving cancer cells of citrate using gluconate, a specific inhibitor of pmCiC, significantly reduced the growth and metastatic spread of human pancreatic cancer cells in vivo and muted stromal activation and angiogenesis. We conclude that citrate is supplied to tumour cells by CAS and citrate uptake plays a significant role in cancer metastatic progression.

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Section: Methodsmentioning

confidence: 99%

Cancer-associated cells release citrate to support tumour metastatic progression

Drexler

Schmidt²,

Jordan³

et al. 2021

Life Sci. Alliance

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“…The Chick Chorioallantoic Membrane (CAM) model was performed as described before [ 11 , 26 , 27 ]. Two days after implantation of 2 × 10 6 Merkel cell carcinoma cells (WaGa and MKL-1), daily treatment with either Na + -gluconate (500 mg/kg/d) or NaCl 0.9% was performed ( n = 20).…”

Section: Methodsmentioning

confidence: 99%

The Role of Citrate Homeostasis in Merkel Cell Carcinoma Pathogenesis

Drexler

Schwertner

Aung

et al. 2022

Cancers

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Merkel cell carcinoma (MCC) is a rare but highly aggressive tumor of the skin with a poor prognosis. The factors driving this cancer must be better understood in order to discover novel targets for more effective therapies. In the search for targets, we followed our interest in citrate as a central and critical metabolite linked to fatty acid synthesis in cancer development. A key to citrate uptake in cancer cells is the high expression of the plasma membrane citrate transporter (pmCiC), which is upregulated in the different adenocarcinoma types tested so far. In this study, we show that the pmCiC is also highly expressed in Merkel cell carcinoma cell lines by western blot and human tissues by immunohistochemistry staining. In the presence of extracellular citrate, MCC cells show an increased proliferation rate in vitro; a specific pmCiC inhibitor (Na+-gluconate) blocks this citrate-induced proliferation. Furthermore, the 3D in vivo Chick Chorioallantoic Membrane (CAM) model showed that the application of Na+-gluconate also decreases Merkel cell carcinoma growth. Based on our results, we conclude that pmCiC and extracellular citrate uptake should be considered further as a potential novel target for the treatment of Merkel cell carcinoma.

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“…The CAM model was performed as previously described [ 24 , 25 , 26 , 27 ]. Upon arrival, chicken eggs were disinfected with ethanol (70%) and placed in a ProCon egg incubator (Grumbach, Asslar, Germany) at 37.8 °C and 63% humidity in combination with hourly rotation.…”

Section: Methodsmentioning

confidence: 99%

“…LSCI is a semi-quantitative method which can be used to measure microcirculatory blood perfusion based on the fluctuations of the contrast in a speckle pattern that is caused by the backscattering of laser arrays due to the movement of erythrocytes in the blood vessels. The PeriCAM perfusion speckle imager (PSI) system high resolution (HR) model (PERIMED, Netherlands) was used for LSCI measurements adjacent to the previously described protocol, and the average perfusion rate was calculated by the PimSoft version 1.5 software [ 25 ]. Minor deviations from the previously described protocol included an increased sampling frequency of 44 Hz, which increased the number of images that were used to calculate an average for one measurement to 88.…”

Section: Methodsmentioning

confidence: 99%

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A 3D In Vivo Model for Studying Human Renal Cystic Tissue and Mouse Kidney Slices

Bichlmayer

Mahl

Hesse

et al. 2022

Cells

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(1) Background: Autosomal dominant polycystic kidney disease (ADPKD) is a frequent monogenic disorder that leads to progressive renal cyst growth and renal failure. Strategies to inhibit cyst growth in non-human cyst models have often failed in clinical trials. There is a significant need for models that enable studies of human cyst growth and drug trials. (2) Methods: Renal tissue from ADPKD patients who received a nephrectomy as well as adult mouse kidney slices were cultured on a chorioallantoic membrane (CAM) for one week. The cyst volume was monitored by microscopic and CT-based applications. The weight and angiogenesis were quantified. Morphometric and histological analyses were performed after the removal of the tissues from the CAM. (3) Results: The mouse and human renal tissue mostly remained vital for about one week on the CAM. The growth of cystic tissue was evaluated using microscopic and CT-based volume measurements, which correlated with weight and an increase in angiogenesis, and was accompanied by cyst cell proliferation. (4) Conclusions: The CAM model might bridge the gap between animal studies and clinical trials of human cyst growth, and provide a drug-testing platform for the inhibition of cyst enlargement. Real-time analyses of mouse kidney tissue may provide insights into renal physiology and reduce the need for animal experiments.

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Laser speckle contrast analysis (LASCA) technology for the semiquantitative measurement of angiogenesis in in-ovo-tumor-model

Cited by 20 publications

References 48 publications

Cancer-associated cells release citrate to support tumour metastatic progression

Cancer-associated cells release citrate to support tumour metastatic progression

The Role of Citrate Homeostasis in Merkel Cell Carcinoma Pathogenesis

A 3D In Vivo Model for Studying Human Renal Cystic Tissue and Mouse Kidney Slices

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