Laser scanning cytometry for the detection of neoplasia in urologic cytology specimens

Foster, Sarah S.; Leiman, Gladwyn; Schwarz, Janet E.; John, Timothy St.; Beatty, Barbara

doi:10.1002/cncr.20112

Cited by 3 publications

(5 citation statements)

References 65 publications

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“…Schiff's reagent covalently binds DNA in a quantitative way (Chieco and Derenzini, 1999; Hardie et al. , 2002), and propidium iodide has also been used to calculate nuclear DNA content (Foster et al. , 2004; Grundler et al.…”

Section: Discussionmentioning

confidence: 99%

“…Feulgen staining allows static cytometry to determine the DNA content of nuclei DNA can be quantitated by staining DNA with a fluorescent dye, followed by measuring the fluorescence emitted from stained samples. Schiff's reagent covalently binds DNA in a quantitative way (Chieco and Derenzini, 1999;Hardie et al, 2002), and propidium iodide has also been used to calculate nuclear DNA content (Foster et al, 2004;Grundler et al, 2004;Kuta et al, 2004). We used both these dyes to analyse the DNA content of individual nuclei in maize ovaries.…”

Section: Discussionmentioning

confidence: 99%

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Confocal microscopy of whole ovules for analysis of reproductive development: the elongate1 mutant affects meiosis II

Barrell

Grossniklaus²

2005

The Plant Journal

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Analysis of female meiosis (megasporogenesis) and embryo sac development (megagametogenesis) in angiosperms is technically challenging because the cells are enclosed within the nucellus and ovule tissues of the female flower. This is in contrast to male sporogenesis and gametogenesis where development can readily be observed through the easily dissectable developing anthers. Observation of embryo sac development is a particular problem in crassinucellate ovules such as those of maize. To overcome the problems in observing reproductive development, we developed a simple Feulgen staining procedure optimized for use with confocal microscopy to observe reproductive progression in the crassinucellate ovules of maize. The procedure greatly facilitates the observation of nuclei and cell structures of all stages of megasporogenesis and embryo sac development. The high resolution obtained using the technique enabled us to readily visualize chromosomes from individual cells within ovule tissue samples of maize. A propidium iodide staining technique was also used and compared with the Feulgen-based technique. Static cytometry of relative DNA content of individual nuclei was possible using Imaris software on both Feulgen and propidium iodide-stained samples. The techniques also proved successful for the observation of Arabidopsis and Hieracium aurantiacum female gametophyte and seed development, demonstrating the general applicability of the techniques. Using both staining methods, we analysed the maize meiotic mutant elongate1, which produces functional diploid instead of haploid embryo sacs. The precise defect in meiosis from which diploid embryo sacs arise in elongate1 has not previously been reported. We used confocal microscopy followed by static cytometry using Imaris software to show that the defect by which diploid embryo sacs arise in the maize mutant elongate1 is the absence of meiosis II with one of the dyad cells directly initiating megagametogenesis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Confocal microscopy of whole ovules for analysis of reproductive development: the elongate1 mutant affects meiosis II

Barrell

Grossniklaus²

2005

The Plant Journal

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“…The laser-excited fluorescence emitted from individual fluorochromed cells on a microscope slide is rapidly measured at multiple wavelengths with high sensitivity and accuracy. LSC has a number of applications, including the measurement of circulating tumor cells, DNA damage, DNA ploidy and cell-cycle position (19)(20)(21)(22)(23)(24)(25)(26)(27). The cells for the study are selected by the operator in a manner that is similar to that for an image analysis system.…”

Section: Discussionmentioning

confidence: 99%

“…Alternatively, the entire cell population on the slide may be measured automatically using small samples, including biopsy specimens. Although flow cytometric analysis requires a large number of cells, LSC may be performed with a relatively small number (17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28).…”

Section: Discussionmentioning

confidence: 99%

Prognostic significance of S-phase fractions in peritumoral invading zone analyzed by laser scanning cytometry in patients with high-grade glioma: A preliminary study

et al. 2016

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Abstract. The predominant characteristic of malignant glioma is the presence of invading tumor cells in the peritumoral zone. Distinguishing between tumor cells and normal cells in a peritumoral lesion is challenging. Therefore, the aim of the present study was to investigate the cell-cycle phase measurements of fixed paraffin-embedded specimens from the peritumoral invading zone of high-grade gliomas using laser scanning cytometry. A total of 12 high-grade gliomas (2 anaplastic astrocytomas and 10 glioblastomas) were studied. The tumor core and peritumoral invading zone of each tumor specimen were investigated. Tissue sections (50 µm) from the paraffin blocks were deparaffinized, rehydrated and enzymatically disintegrated, and the cells in suspension were stained with propidium iodide and placed on microscope slides. A slight trend for an increased S-phase fraction in the peritumoral invading zone compared with the tumor core was observed (P=0.24). Additionally, there was a trend for a decrease in the overall survival time of patients with increasing peritumoral invading zone S-phase fraction (P=0.12). These data suggest that laser scanning cytometry is a powerful and clinically relevant tool for the objective analysis of the cell cycle in malignant gliomas.

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“…Consequently, this technique has been successfully applied to surgical tricky regions such as the parotid gland (Gerstner et al [32]), the larynx (Gerstner et al [33]), the oral cavity (Remmerbach et al [34]), and the entire upper aerodigestive tract (Gerstner et al [35]). Assays have also been adopted to detect urologic neoplasia in cytologic specimens (Foster et al [36]). These analyses were based on the DNA-ploidy of the tumor cells and showed very high sensitivity and specificity for cancer detection.…”

Section: Clinical Applicationsmentioning

confidence: 99%

Clinical applications of slide‐based cytometry – an update

Gerstner

Laffers

Tárnok

2009

Journal of Biophotonics

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Slide-based cytometric approaches open the possibility to obtain quantitative and objective data from specimens that so far have not been accessible to this kind of analysis. In this review, we will highlight the specific advantages of slide-based cytometry (SBC) and show the applications that have been established for clinical samples. Focuses are cytomic analyses of oncological and hematological samples where the slide-based concept turned out to open new dimensions in understanding underlying cellular networks. We review the recent literature and point out future applications.

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Laser scanning cytometry for the detection of neoplasia in urologic cytology specimens

Cited by 3 publications

References 65 publications

Confocal microscopy of whole ovules for analysis of reproductive development: the elongate1 mutant affects meiosis II

Confocal microscopy of whole ovules for analysis of reproductive development: the elongate1 mutant affects meiosis II

Prognostic significance of S-phase fractions in peritumoral invading zone analyzed by laser scanning cytometry in patients with high-grade glioma: A preliminary study

Clinical applications of slide‐based cytometry – an update

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