Laser Raman Spectroscopic Studies on Hemeproteins in Epileptic Children

Abstract

Laser Raman spectroscopic studies were carried out on hemoproteins with special reference to epilepsy and compared the data with those of controls. Some of the bands were found approximately at 368.45 cm −1 , 424.90 cm −1 , 625.27 cm −1 and 807.38 cm −1 in case of normal children and at 1749.00 cm −1 , 1795 cm −1 and 2000 cm −1 in epileptic children cases. A clear cut picture of the hemoproteins has already given in the literature and very interesting bands were found in the range from 300 cm −1 to 1800 cm −1 . Our Raman lines are very effective and peculiar. We did not say anything about the detailing of these bands at this juncture.

Substrate-Independent Control of Polymorphs in Tetraphenylporphyrin Thin Films by Varying the Solvent Evaporation Time Using a Simple Spin-Coating Technique

“…Using this value, the film thickness, d , was estimated from the absorbance ratio of analytes to the standard film, A iso analyte / A iso standard , as shown in eq . Here, A iso means an “orientation-free” spectrum generated by taking the average of the MAIRS-IP and -OP spectra as shown in eq . , The band intensity of an orientation-free spectrum is directly proportional to the quantity of the chemical species. , This method should thus work properly for the estimation of thickness as long as the molecular density is not so different from the standard sample. For the calculation, the most intense band of H 2 TPP at about 800 cm –1 that is assigned to the C–H out-of-plane deformation vibration mode of the porphyrin ring (denoted as γ­(C–H) por ) ,− was used.…”

Raman Spectroscopy of Blood and Blood Components

“…Finally, recent Raman spectroscopy studies have investigated heme proteins from the blood of children with epilepsy, 115 and metHb has been measured as a clinical biomarker of pathophysiology, such as in nitrite poisoning. 116,117…”

“…With the aid of multivariate statistical methods (and analysis of the high-wavenumber region of the spectrum), they achieved a diagnostic accuracy of 98.8% for differentiating diabetic from normal RBC membranes. 114 Finally, recent Raman spectroscopy studies have investigated heme proteins from the blood of children with epilepsy, 115 and metHb has been measured as a clinical biomarker of pathophysiology, such as in nitrite poisoning. 116,117 White Blood Cells White blood cells are part of the body's immune system, and are responsible for fighting bacterial, viral, and fungal infections.…”