Laser pulse impact on rat mesenteric blood vessels in relation to laser treatment of port wine stain

Verkruysse, Wim; Beek, Hans van; vanBavel, Ed; Gemert, Martin J. C. van; Spaan, Jos A. E.

doi:10.1002/lsm.1075

Cited by 11 publications

(4 citation statements)

References 28 publications

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“…2a) 7,15,19,21,22,107,150. By irradiating skin at a well-absorbed wavelength and sufficient irradiance (the amount of photon energy incident on a unit area of tissue per unit time, expressed in W/cm 2 ), supracritical temperatures (>70 °C) can be generated in the vessel lumen and confined spatially if the pulse duration is kept within the thermal relaxation time of the target blood vessel or, in case of large-diameter vasculature, the volume of blood in which the radiant energy is absorbed.…”

Section: Endovascular Laser–tissue Interactionsmentioning

confidence: 99%

An Overview of Three Promising Mechanical, Optical, and Biochemical Engineering Approaches to Improve Selective Photothermolysis of Refractory Port Wine Stains

et al. 2011

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During the last three decades, several laser systems, ancillary technologies, and treatment modalities have been developed for the treatment of port wine stains (PWSs). However, approximately half of the PWS patient population responds suboptimally to laser treatment. Consequently, novel treatment modalities and therapeutic techniques/strategies are required to improve PWS treatment efficacy. This overview therefore focuses on three distinct experimental approaches for the optimization of PWS laser treatment. The approaches are addressed from the perspective of mechanical engineering (the use of local hypobaric pressure to induce vasodilation in the laser-irradiated dermal microcirculation), optical engineering (laser-speckle imaging of post-treatment flow in laser-treated PWS skin), and biochemical engineering (light- and heat-activatable liposomal drug delivery systems to enhance the extent of post-irradiation vascular occlusion).

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Section: Endovascular Laser–tissue Interactionsmentioning

confidence: 99%

An Overview of Three Promising Mechanical, Optical, and Biochemical Engineering Approaches to Improve Selective Photothermolysis of Refractory Port Wine Stains

et al. 2011

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“…In particular, in one study, rat mesenteric blood vessels were irradiated with a laser pulse (585 nm, 0.2-0.6 ms pulse duration, 0.5-30 J/cm 2 radiant exposure) [147] . Video microscopy was used to assess vessel dilation, formation of intravascular thrombi, bubble formation, and vessel rupture.…”

Section: High-power Laser Treatment Of Vascular Abnormalitiesmentioning

confidence: 99%

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

Galanzha¹

2007

WJG

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Using animal mesentery with intravital optical microscopy is a well-established experimental model for studying blood and lymph microcirculation in vivo . Recent advances in cell biology and optical techniques provide the basis for extending this model for new applications, which should generate significantly improved experimental data. This review summarizes the achievements in this specific area, including in vivo label-free blood and lymph photothermal flow cytometry, super-sensitive fluorescence image cytometry, light scattering and speckle flow cytometry, microvessel dynamic microscopy, infrared (IR) angiography, and high-speed imaging of individual cells in fast flow. The capabilities of these techniques, using the rat mesentery model, were demonstrated in various studies; e.g., real-time quantitative detection of circulating and migrating individual blood and cancer cells, studies on vascular dynamics with a focus on lymphatics under normal conditions and under different interventions (e.g. lasers, drugs, nicotine), assessment of lymphatic disturbances from experimental lymphedema, monitoring cell traffic between blood and lymph systems, and high-speed imaging of cell transient deformability in flow. In particular, the obtained results demonstrated that individual cell transportation in living organisms depends on cell type (e.g., normal blood or leukemic cells), the cell's functional state (e.g., live, apoptotic, or necrotic), and the functional status of the organism. Possible future applications, including in vivo early diagnosis and prevention of disease, monitoring immune response and apoptosis, chemo-and radio-sensitivity tests, and drug screening, are also discussed. TOPIC HIGHLIGHT

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“…The acute and chronic inflammatory responses triggered by laser-induced endovascular damage lead to vascular remodeling processes that result in lesional clearance. The exact mechanisms underlying the (endo)vascular responses to laser irradiation have not been extensively elucidated, primarily due to the fact that only few in vivo techniques have been developed to categorically examine these phenomena [14][15][16][17][18]. Moreover, most of these laserinduced vessel wall injury models [14][15][16] focus the laser beam directly on the endothelial layer, creating a damage profile that differs from selective photothermolysis in that the chromophore-containing red blood cells are circumvented as thermal catalysts and heat diffusion is an irrelevant factor.…”

Section: Introductionmentioning

confidence: 99%

Darkfield orthogonal polarized spectral imaging for studying endovascular laser-tissue interactions in vivo-a preliminary study

et al. 2005

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Due to the limited number of suitable intravital microscopy techniques, relatively little is known about the opto-thermal (endo)vascular responses to selective photothermolysis, used as a default treatment modality for superficial vascular anomalies such as port wine stains, telangiectasias, and hemangiomas. In this preliminary study we present a novel microscopy technique for studying (endo)vascular laser-tissue interactions in vivo, in which conventional orthogonal polarized spectral (OPS) imaging is combined with darkfield (DF) illumination. DFOPS imaging of rat mesenteric vasculature irradiated at increasing powers revealed the following (tissular) responses: formation of translucent aggregates, retrograde flow, gradual and immediate hemostasis, reinstatement of flow, vessel disappearance, and perivascular collagen damage. DFOPS imaging therefore constitutes a useful tool for examining (endo)vascular events following selective photothermolysis. W. Groner, J. W. Winkelman, A. G. Harris, C. Ince, G. J. Bouma, K. Messmer, and R. G. Nadeau, "Orthogonal polarization spectral imaging: a new method for study of the microcirculation," Nat. Med. 5, 1209-1212 (1999

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Laser pulse impact on rat mesenteric blood vessels in relation to laser treatment of port wine stain

Cited by 11 publications

References 28 publications

An Overview of Three Promising Mechanical, Optical, and Biochemical Engineering Approaches to Improve Selective Photothermolysis of Refractory Port Wine Stains

An Overview of Three Promising Mechanical, Optical, and Biochemical Engineering Approaches to Improve Selective Photothermolysis of Refractory Port Wine Stains

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

Darkfield orthogonal polarized spectral imaging for studying endovascular laser-tissue interactions in vivo-a preliminary study

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