Laser light scattering immunoassay: An improved data analysis by CONTIN method

Antony, Thomas; Saxena, Anita; Roy, Kunal; Bohidar, H. B.

doi:10.1016/s0165-022x(97)00047-x

Cited by 25 publications

(10 citation statements)

References 11 publications

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“…To determine the hydrodynamic diameter, dynamic light scattering (DLS) measurements of Pdots in an aqueous solution were performed using a 633 nm laser source (Figure S7). The average diameter of the green-, red-, and NIR-emissive Pdots was ∼60 nm according to the constrained regularization method for inverting data (CONTIN) analysis . As shown in Figure S7d, all Pdots show negative zeta potentials, ranging from −37 to −52 mV because of the presence of negatively charged carboxylate groups on the Pdot surfaces. , No aggregation or significant changes in the optical properties were observed for the three types of Pdots, even after several months, indicating the pronounced stability of the dispersed Pdots in aqueous media.…”

Section: Results and Discussionmentioning

confidence: 99%

Green-, Red-, and Near-Infrared-Emitting Polymer Dot Probes for Simultaneous Multicolor Cell Imaging with a Single Excitation Wavelength

et al. 2020

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We report newly synthesized fluorescence resonance energy transfer (FRET)-based green-, red-, and near-infrared (NIR)-emitting polymer dot (Pdot) probes. Fluorescent Pdots (∼60 nm) were prepared with a green-emissive conjugated polymer (PPDT-P, donor) alone or mixing the donor with a red-or NIRemitting fluorophore (T-DCS or ITIC, acceptor), where an optically inert matrix polymer [poly(styrene-co-maleic anhydride)] was mixed together to minimize the aggregation-caused quenching by diluting the fluorophores and surface functionalization for further bioconjugation with antibodies for active targeting. Highly fluorescent green-emissive PPDT-P Pdots were prepared with a photoluminescence (PL) quantum efficiency of ∼30% and the FRETmediated red and NIR PL was intensified by 5.3−8.5 times (with high FRET ratios of ∼9) via the efficient energy transfer (FRET efficiency of 80−98%) and antenna effect, compared with the signals obtained via direct excitation of the fluorophores in Pdots. All three types of Pdot/antibody conjugates were simultaneously immunostained to COS-7 cells (showing minimal cross-reactivity), reducing the tedious sequential immunostaining process to a single step. Finally, we obtained high-contrast three-color cell images with little spectral leakthrough by exciting all the probes simultaneously at a single wavelength at 405 nm without the need for a complicated or expensive multiple-excitation setup.

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Section: Results and Discussionmentioning

confidence: 99%

Green-, Red-, and Near-Infrared-Emitting Polymer Dot Probes for Simultaneous Multicolor Cell Imaging with a Single Excitation Wavelength

et al. 2020

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“…Vega and coworkers proposed two methods to obtain latex particle size distribution by DLS [10,11]. Antony et al reported the feasibility of DLS to detect agglutination of antigen-carrier particles mediated by antibody [12]. However, these treatments lack in the consideration of the q-dependence of the solutes, i.e., the intensity contribution to the time-correlation function via S(q).…”

Section: Introductionmentioning

confidence: 99%

Distribution analyses of multi-modal dynamic light scattering data

Shibayama

Karino

Okabe

2006

Polymer

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“…The correlation function was accepted when the difference between the measured and the calculated baseline was less than 0.1%. CONTIN algorithms were used in the Laplace inversion of the autocorrelation function to obtain the size distribution of micelles (Antony et al, 1998). The morphology of TCH PMs was analyzed by high resolution transmission electron microscopy (JEOL 300 kV, USA).…”

Section: Preparation Of Bfgf Loaded Polymeric Micellementioning

confidence: 99%

Heparin-Conjugated Nanointerfaces for Biomedical Applications

Парк

Joung

2009

NanoScience in Biomedicine

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This chapter describes some examples of heparin-conjugated nanointerfaces for biomedical applications in terms of both development and application. First, heparin-conjugated linear and star shaped poly(lactic acid) (PLA-Hep and sPLA-Hep) to improve blood compatibility and its related biocompatibility of degradable polymers were introduced. Second, a heparin-conjugated PM system that consists of Tetronic and poly( -caprolactone) (PCL) is described as an injectable vehicle for an effective long-term delivery of growth factors. Finally, the heparin-immobilized small intestinal submucosa (SIS) was investigated to observe the effect of heparin immobilization on blood compatibility, in vitro fibroblast attachment and in vivo calcification of SIS. All of these examples demonstrate that heparin-conjugated nanointerfaces might be a versatile and promising tool to achieve advanced biomaterials.

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Laser light scattering immunoassay: An improved data analysis by CONTIN method

Cited by 25 publications

References 11 publications

Green-, Red-, and Near-Infrared-Emitting Polymer Dot Probes for Simultaneous Multicolor Cell Imaging with a Single Excitation Wavelength

Green-, Red-, and Near-Infrared-Emitting Polymer Dot Probes for Simultaneous Multicolor Cell Imaging with a Single Excitation Wavelength

Distribution analyses of multi-modal dynamic light scattering data

Heparin-Conjugated Nanointerfaces for Biomedical Applications

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