Laser irradiation of the guinea pig basilar membrane

Wenzel, Gentiana I.; Pikkula, Brian M.; Choi, Chul-Hee; Anvari, Bahman; Oghalai, John S.

doi:10.1002/lsm.20091

Cited by 18 publications

(13 citation statements)

References 26 publications

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“…We could refer to our animal study results that showed no adverse effects from transmeatal irradiation of 144 J/cm 2 (80 mW/cm 2 x 30 min). It has been reported that LLL irradiation reduced the birefringence within the basilar membrane as well as within other collagen-containing structures of cochlea 16 . The report concluded that these alterations of basement membrane by LLL therapy may affect cochlear tuning and it may benefit for patients with high-frequency sensorineural hearing loss.…”

Section: Discussionmentioning

confidence: 99%

Effect of low level laser (LLL) on cochlear and vestibular inner ear including tinnitus

et al. 2006

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Objectives: 1. To investigate preventive effect of LLL on gentamicin-induced vestibular ototoxicity.2. To evaluate the effectiveness of lower level laser (LLL) in the treatment of tinnitus. Methods: 1.Twenty guinea pigs were divided into control and laser groups. Vestibular ototoxicity was induced by intratympanic injection of gentamicin into left ear. LLL was irradiated into left ear canal of animals in laser group. Vestibular function of the animals was evaluated with vertical and offvertical axis rotation testing. 2. Forty patients with tinnitus were treated with ginkgo biloba orally and randomly divided into control and laser groups. The 20 patients of laser group received 80.4 J/cm 2 of 830 nm laser, 3 times per week for 4 weeks, via transmeatal irradiation. Tinnitus was evaluated by visual analogue scale (VAS) and tinnitus handicap inventory (THI). Results: 1. Preventive effect of LLL to gentamicin induced vestibular ototoxicity was demonstrated by preventing reduction of gain in slow harmonic acceleration test and modulation in the off-vertical axis rotation test. 2. Eleven of 20 laser group patients have shown significant improvement in VAS and THI compared to those of the control group. Conclusions: 1. LLL therapy may have preventive effect to vestibular ototoxicity. 2. LLL therapy in combination with ginkgo biloba seems to be worth trying on patients with tinnitus.

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Section: Discussionmentioning

confidence: 99%

Effect of low level laser (LLL) on cochlear and vestibular inner ear including tinnitus

et al. 2006

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“…We believe this to be a reasonable assumption. However, our model does not consider other changes in cochlear biomechanics that may also occur, such as an increase in CP stiffness (Wenzel et al, 2004) and a decrease in ST fluid mass (de Boer, 1996).…”

Section: Discussionmentioning

confidence: 99%

Predicting the effect of post-implant cochlear fibrosis on residual hearing

2005

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Intracochlear scarring is a well-described sequela of cochlear implantation. We developed a mathematical model of passive cochlear mechanics to predict the impact that this might have upon residual acoustical hearing after implantation. The cochlea was modeled using lumped impedance terms for scala vestibuli (SV), scala tympani (ST), and the cochlear partition (CP). The damping of ST and CP was increased in the basal one half of the cochlea to simulate the effect of scar tissue. We found that increasing the damping of the ST predominantly reduced basilar membrane vibrations in the apex of the cochlea while increasing the damping of the CP predominantly reduced basilar membrane vibrations in the base of the cochlea. As long as intracochlear scarring continues to occur with cochlear implantation, there will be limitations on hearing preservation. Newer surgical techniques and electrode technologies that do not result in as much scar tissue formation will permit improved hearing preservation.

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“…Ren et al used PLM to examine microcirculation in the guinea pig cochlea, 15 whereas Wenzel et al used PLM to examine collagen organization and content within the basilar membrane and other cochlear structures after laser irradiation. 16,17 One of the major drawbacks of standard PLM is that it is not a quantitative technique. The intensity of light observed when a birefringent material is viewed by a polarized light microscope depends on the angle of that material's optic axis relative to the transmission axes of the polarization filters.…”

Section: Introductionmentioning

confidence: 99%

Quantitative polarized light microscopy of unstained mammalian cochlear sections

et al. 2013

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Abstract. Hearing loss is the most common sensory deficit in the world, and most frequently it originates in the inner ear. Yet, the inner ear has been difficult to access for diagnosis because of its small size, delicate nature, complex three-dimensional anatomy, and encasement in the densest bone in the body. Evolving optical methods are promising to afford cellular diagnosis of pathologic changes in the inner ear. To appropriately interpret results from these emerging technologies, it is important to characterize optical properties of cochlear tissues. Here, we focus on that characterization using quantitative polarized light microscopy (qPLM) applied to unstained cochlear sections of the mouse, a common animal model of human hearing loss. We find that the most birefringent cochlear materials are collagen fibrils and myelin. Retardance of the otic capsule, the spiral ligament, and the basilar membrane are substantially higher than that of other cochlear structures. Retardance of the spiral ligament and the basilar membrane decrease from the cochlear base to the apex, compared with the more uniform retardance of other structures. The intricate structural details revealed by qPLM of unstained cochlear sections ex vivo strongly motivate future application of polarization-sensitive optical coherence tomography to human cochlea in vivo.

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Laser irradiation of the guinea pig basilar membrane

Cited by 18 publications

References 26 publications

Effect of low level laser (LLL) on cochlear and vestibular inner ear including tinnitus

Effect of low level laser (LLL) on cochlear and vestibular inner ear including tinnitus

Predicting the effect of post-implant cochlear fibrosis on residual hearing

Quantitative polarized light microscopy of unstained mammalian cochlear sections

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