Laser-induced tissue hyperthermia mediated by gold nanoparticles: toward cancer phototherapy

Terentyuk, Georgy S.; Maslyakova, Galina N.; Suleymanova, Leyla V.; Khlebtsov, N. G.; Khlebtsov, Boris N.; Akchurin, Garif G.; Maksimova, Irina L.; Tuchin, Valery V.

doi:10.1117/1.3122371

Cited by 203 publications

(150 citation statements)

References 36 publications

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“…In these studies, the absorption properties of gold nanorods in the surface plasmon resonance wavelength are used to elevate temperature to 50°C and above, usually using lasers with a high optical output (above 10 W/cm 2 for about 20 minutes) in order to achieve effective denaturation and coagulation of cellular proteins, as well as cell death. 11,12,20,21 An in vitro proof of concept for photothermal imaging using targeted gold nanoparticles (GNPs) is demonstrated in this paper. By selectively increasing the temperature of GNPs that specifically target and decorate the surface of cancer cells, we can distinguish between cancerous and noncancerous cells (ie, normal background tissue).…”

Section: Introductionmentioning

confidence: 99%

Towards real-time detection of tumor margins using photothermal imaging of immune-targeted gold nanoparticles

Jakobsohn¹,

Motiei²,

Sinvani³

et al. 2012

IJN

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Background:One of the critical problems in cancer management is local recurrence of disease. Between 20% and 30% of patients who undergo tumor resection surgery require reoperation due to incomplete excision. Currently, there are no validated methods for intraoperative tumor margin detection. In the present work, we demonstrate the potential use of gold nanoparticles (GNPs) as a novel contrast agent for photothermal molecular imaging of cancer. Methods: Phantoms containing different concentrations of GNPs were irradiated with continuous-wave laser and measured with a thermal imaging camera which detected the temperature field of the irradiated phantoms. Results:The results clearly demonstrate the ability to distinguish between cancerous cells specifically targeted with GNPs and normal cells. This technique, which allows highly sensitive discrimination between adjacent low GNP concentrations, will allow tumor margin detection while the temperature increases by only a few degrees Celsius (for GNPs in relevant biological concentrations). Conclusion:We expect this real-time intraoperative imaging technique to assist surgeons in determining clear tumor margins and to maximize the extent of tumor resection while sparing normal background tissue.

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Section: Introductionmentioning

confidence: 99%

Towards real-time detection of tumor margins using photothermal imaging of immune-targeted gold nanoparticles

Jakobsohn¹,

Motiei²,

Sinvani³

et al. 2012

IJN

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“…[3][4][5][6] Furthermore, gold nanoparticles have been investigated in various contexts in relation to cancer diagnosis and treatment, including as a delivery vehicle for several chemotherapeutic agents, as a contrast agent for enhanced imaging, and for thermal ablation therapy. [7][8][9][10][11] Utilizing the differential absorption coefficient of a high atomic number (Z) material, such as gold, compared with soft tissue has shown potential as a radiosensitizing strategy through increased local dose deposition as a result of the generation of Dovepress submit your manuscript | www.dovepress.com secondary electron species. [12][13][14] A tissue modeling study using gold foil reported a dose enhancement factor of 50 due to the production of secondary electrons generated from low-energy photon irradiation.…”

Section: Introductionmentioning

confidence: 99%

Cell type-dependent uptake, localization, and cytotoxicity of 1.9 nm gold nanoparticles

Coulter¹,

Jain²,

Butterworth³

et al. 2012

IJN

154

134

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Background: This follow-up study aims to determine the physical parameters which govern the differential radiosensitization capacity of two tumor cell lines and one immortalized normal cell line to 1.9 nm gold nanoparticles. In addition to comparing the uptake potential, localization, and cytotoxicity of 1.9 nm gold nanoparticles, the current study also draws on comparisons between nanoparticle size and total nanoparticle uptake based on previously published data. Methods: We quantified gold nanoparticle uptake using atomic emission spectroscopy and imaged intracellular localization by transmission electron microscopy. Cell growth delay and clonogenic assays were used to determine cytotoxicity and radiosensitization potential, respectively. Mechanistic data were obtained by Western blot, flow cytometry, and assays for reactive oxygen species. Results: Gold nanoparticle uptake was preferentially observed in tumor cells, resulting in an increased expression of cleaved caspase proteins and an accumulation of cells in sub G 1 phase. Despite this, gold nanoparticle cytotoxicity remained low, with immortalized normal cells exhibiting an LD 50 concentration approximately 14 times higher than tumor cells. The surviving fraction for gold nanoparticle-treated cells at 3 Gy compared with that of untreated control cells indicated a strong dependence on cell type in respect to radiosensitization potential. Conclusion: Gold nanoparticles were most avidly endocytosed and localized within cytoplasmic vesicles during the first 6 hours of exposure. The lack of significant cytotoxicity in the absence of radiation, and the generation of gold nanoparticle-induced reactive oxygen species provide a potential mechanism for previously reported radiosensitization at megavoltage energies.

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“…A variety of different cancer therapeutic strategies have focused on the use of gold nanoparticles including the targeted delivery of anti-cancer agents including methotrexate [5] tamoxifen [6] and oxaliplatin [7], radio frequency radiation [8], thermal ablation [9,10] and metal enhanced radiotherapy [11].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of cytotoxicity and radiation enhancement using 1.9 nm gold particles: potential application for cancer therapy

Butterworth¹,

Coulter²,

Jain³

et al. 2010

Nanotechnology

208

160

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High atomic number (Z) materials such as gold preferentially absorb kilovoltage x-rays compared to soft tissue and may be used to achieve local dose enhancement in tumours during treatment with ionizing radiation. Gold nanoparticles have been demonstrated as radiation dose enhancing agents in vivo and in vitro. In the present study, we used multiple endpoints to characterize the cellular cytotoxic response of a range of cell lines to 1.9 nm gold particles and measured dose modifying effects following transient exposure at low concentrations. Gold nanoparticles caused significant levels of cell type specific cytotoxicity, apoptosis and increased oxidative stress. When used as dose modifying agents, dose enhancement factors varied between the cell lines investigated with the highest enhancement being 1.9 in AGO-1522B cells at a nanoparticle concentration of 100 μg ml −1 . This study shows exposure to 1.9 nm gold particles to induce a range of cell line specific responses including decreased clonogenic survival, increased apoptosis and induction of DNA damage which may be mediated through the production of reactive oxygen species. This is the first study involving 1.9 nm nanometre sized particles to report multiple cellular responses which impact on the radiation dose modifying effect. The findings highlight the need for extensive characterization of responses to gold nanoparticles when assessing dose enhancing potential in cancer therapy.

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Laser-induced tissue hyperthermia mediated by gold nanoparticles: toward cancer phototherapy

Cited by 203 publications

References 36 publications

Towards real-time detection of tumor margins using photothermal imaging of immune-targeted gold nanoparticles

Towards real-time detection of tumor margins using photothermal imaging of immune-targeted gold nanoparticles

Cell type-dependent uptake, localization, and cytotoxicity of 1.9 nm gold nanoparticles

Evaluation of cytotoxicity and radiation enhancement using 1.9 nm gold particles: potential application for cancer therapy

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