Laser-induced thermal stress and the heat shock response in neural cells

Emohare, Osa; Hafez, M.I.; Sandison, Ann; Coombs, Richard; McCarthy, Ian

doi:10.1080/00016470410001510

Cited by 3 publications

(3 citation statements)

References 11 publications

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“…The Holmium:YAG laser is extensively used in orthopedic surgeries, and efforts to characterize the effects of the laser on cell viability yielded intriguing results; shock waves and thermal shock caused by the laser-induced cell expression of Hsp70 [180]. As the Hsp family of molecules has been implicated in cytoprotection and immune modulation, laser preconditioning of stem and progenitor cells could provide a means of increasing their paracrine actions.…”

Section: Modulating Stem Cell Paracrine Actionsmentioning

confidence: 99%

Stem Cell Paracrine Actions and Tissue Regeneration

2009

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Stem cells have emerged as a key element of regenerative medicine therapies due to their inherent ability to differentiate into a variety of cell phenotypes, thereby providing numerous potential cell therapies to treat an array of degenerative diseases and traumatic injuries. A recent paradigm shift has emerged suggesting that the beneficial effects of stem cells may not be restricted to cell restoration alone, but also due to their transient paracrine actions. Stem cells can secrete potent combinations of trophic factors that modulate the molecular composition of the environment to evoke responses from resident cells. Based on this new insight, current research directions include efforts to elucidate, augment and harness stem cell paracrine mechanisms for tissue regeneration. This article discusses the existing studies on stem/progenitor cell trophic factor production, implications for tissue regeneration and cancer therapies, and development of novel strategies to use stem cell paracrine delivery for regenerative medicine. Keywordscancer; immune modulation; paracrine actions; stem cells; tissue regeneration; trophic factors Regenerative medicine therapies, fueled by advances in stem cell biology and technologies, seek to direct inherent nonhealing injuries towards full restoration of tissue structure and subsequent function. Numerous studies have demonstrated that mobilization of endogenous stem cells or exogenous administration of a number of stem cell populations to injured tissues has resulted in structural regeneration of tissue as well as functional improvement. While the original hypothesis underlying stem cell regenerative therapies was based on functional recovery as a consequence of stem cell differentiation, it is now clear that other mechanisms of action are at play. A recent paradigm shift has emerged suggesting that the biomolecules synthesized by stem cells may be as important, if not more so, than differentiation of the cells in eliciting functional tissue repair. The fate of a stem cell is determined by its niche, or local microenvironment, consisting of surrounding cells and the secreted products of the stem cell [1]. Stem cells actively contribute to their environment by secreting cytokines, growth factors and extracellular matrix (ECM) molecules that act either on themselves (autocrine actions) or

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Section: Modulating Stem Cell Paracrine Actionsmentioning

confidence: 99%

Stem Cell Paracrine Actions and Tissue Regeneration

2009

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“…It is also possible that the heat produces local coagulation of proinflammatory proteins or that heat shock proteins can be induced at the periphery of the treated region, which could then have an anti-inflammatory effect. 16 Results can be compared with output from a theoretical model. 5 In this model, the ultrasound intensity field was approximated by a Gaussian distribution.…”

Section: Discussionmentioning

confidence: 99%

Effects of high‐intensity focused ultrasound on the intervertebral disc: A potential therapy for disc herniations

Forslund¹,

Persson

Strömqvist

et al. 2006

J of Clinical Ultrasound

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“…The high temperature region directly above the heated wires should thus induce cell death while, at short exposure times of a few minutes, the viability of the cells in the surrounding parts should not be affected. 37…”

Section: Temperature Distributionmentioning

confidence: 99%

Inducing microscopic thermal lesions for the dissection of functional cell networks on a chip

et al. 2015

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We present a versatile chip-based method to inflict microscopic lesions on cellular networks or tissue models. Our approach relies on resistive heating of microstructured conductors to impose highly localized thermal stress on specific regions of a cell network. We show that networks can be precisely dissected into individual subnetworks using a microwire crossbar array. To this end, we pattern a network of actively beating cardiomyocyte-like cells into smaller subunits by inflicting thermal damage along selected wires of the array. We then investigate the activity and functional connectivity of the individual subnetworks using a Ca(2+) imaging-based signal propagation analysis. Our results demonstrate the efficient separation of functional activity between individual subnetworks on a microscopic level. We believe that the presented technique may become a powerful tool for investigating lesion and regeneration models in cellular networks.

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Laser-induced thermal stress and the heat shock response in neural cells

Cited by 3 publications

References 11 publications

Stem Cell Paracrine Actions and Tissue Regeneration

Stem Cell Paracrine Actions and Tissue Regeneration

Effects of high‐intensity focused ultrasound on the intervertebral disc: A potential therapy for disc herniations

Inducing microscopic thermal lesions for the dissection of functional cell networks on a chip

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