Laser-Induced Periodic Ag Surface Structure with Au Nanorods Plasmonic Nanocavity Metasurface for Strong Enhancement of Adenosine Nucleotide Label-Free Photoluminescence Imaging

Abstract

The label-free detection of biomolecules by means of fluorescence spectroscopy and imaging is topical. The developed surface-enhanced fluorescence technique has been applied to achieve progress in the label-free detection of biomolecules including deoxyribonucleic acid (DNA) bases. In this study, the effect of a strong enhancement of photoluminescence of 5′-deoxyadenosine-monophosphate (dAMP) by the plasmonic nanocavity metasurface composed of the silver femtosecond laser-induced periodic surface structure (LIPSS) and gold nanorods or nanospheres has been realized at room temperature. The highest value of 1220 for dAMP on the Ag-LIPSS/Au nanorod metasurface has been explained to be a result of the synergetic effect of the generation of hot spots near the sharp edges of LIPSS and Au nanorod tips together with the excitation of collective gap mode of the cavity due to strong near-field plasmonic coupling. A stronger plasmonic enhancement of the phosphorescence compared to the fluorescence is achieved due to a greater overlap of the phosphorescence spectrum with the surface plasmon spectral region. The photoluminescence imaging of dAMP on the metasurfaces shows a high intensity in the blue range. The comparison of Ag-LIPSS/Au nanorod and Ag-LIPSS/Au-nanosphere metasurfaces shows a considerably higher enhancement for the metasurface containing Au nanorods. Thus, the hybrid cavity metasurfaces containing metal LIPSS and nonspherical metal nanoparticles with sharp edges are promising for high-sensitive label-free detection and imaging of biomolecules at room temperature.

Femtosecond Laser Fabrication of Noble Metal Plasma Nanostructures and Its Application-A Mini Review

“…In addition, in 2020, Yeshchenko et al used femtosecond laser-induced silver periodic surface structures and gold nanorods or nanospheres to form plasmonic nanocavities. This structure is expected to be used for high-sensitivity label-free detection and imaging of biomolecules under the greenhouse [44] (Figure 1F).…”

Surface Plasmon-Assisted Fluorescence Enhancing and Quenching: From Theory to Application

“…The nanocarbon materials, for example, carbon nanotube, graphene, and graphene oxide, enable a carbon-based layer to be extensively applied as a spacing matrix, since the rigid framework of a nanocarbon-built matrix can not only place the attached fluorophore at the desired coupling channel in SPCE test but also align and reorientate the attached fluorophores to facilitate the effient exciation and emission through π–π stacking. Moreover, the carbon nanostructure in a nanocavity could largely enhance the emission yield in applications. , The carbon nanotube (CNT) and its nanocomposites, in particular, are often introduced onto a metallic surface as a spacing layer in SPCE measurements . A sensitive immunoassay is accomplished on this CNT spacing matrix as shown in Figure a.…”

“…Moreover, the carbon nanostructure in a nanocavity could largely enhance the emission yield in applications. 155,156 The carbon nanotube (CNT) and its nanocomposites, in particular, are often introduced onto a metallic surface as a spacing layer in SPCE measurements. 157 A sensitive immunoassay is accomplished on this CNT spacing matrix as shown in Figure 16a.…”

Regulating Morphology and Composition of Laser-Induced Periodic Structures on Titanium Films with Femtosecond Laser Wavelength and Ambient Environment

“…Various theoretical approaches have been applied to explain the LIPSS occurrence, including the scattering-interference mechanism, excitation of surface electromagnetic waves (polaritons, plasmons), counter-propagating surface plasmons, and material reorganization [ 3 , 4 , 5 ]. Although a comprehensive theoretical picture is still in development, LIPSS attract research attention due to the remarkable simplicity and reliability of the single-step technique of patterning the surface with subwavelength periodic features suggesting a wide range of possible applications, such as surface colorization, reflection/transmission tuning, wettability and friction control, security marking, energy storage and solar cells efficiency enhancement, biosensing, living cell growth and adhesion control, and more [ 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ].…”