Laser-Induced Forward Transfer with Optical Stamp of a Protein-Immobilized Calcium Phosphate Film Prepared by Biomimetic Process to a Human Dentin

Abstract

The rapid and area-specific printing of calcium phosphate with superior biocompatibility and osteoconductivity is a useful technique for the surface functionalization of biomedical devices. We recently demonstrated the laser-induced forward transfer (LIFT) of a brittle calcium phosphate film onto a soft and shock-absorbing polydimethylsiloxane (PDMS) substrate. In this work, a new LIFT using an optically transparent PDMS-coated stamp, which we hereafter call LIFT with optical stamp (LIFTOP), was introduced to achieve the transfer of brittle films to harder substrates. Cell adhesion protein fibronectin-immobilized calcium phosphate films (Fn-CaP) were prepared on the optical stamp through a biomimetic process. Then, the irradiation of a single laser pulse transferred the Fn-CaP film from the optical stamp onto relatively hard substrates, polyethylene terephthalate and human dentin. As a result of this LIFTOP process, Fn-CaP microchips with a shape corresponding to the laser beam spot were printed on the substrates. Cross-sectional observation of the interface between the Fn-CaP microchip and the dentin substrate revealed good attachment between them without obvious gaps for the most part.

There Are over 60 Ways to Produce Biocompatible Calcium Orthophosphate (CaPO4) Deposits on Various Substrates

“…The LIFT process usually involves the irradiation of a single laser pulse through a transparent support onto a donor material or sacrificial layer that absorbs laser light, which causes heating, melting and ablation, and other laser-induced phenomena. In general, laserinduced changes cause a transient excitation field at high temperature and/or high pressure, thereby moving the donor material toward the acceptor substrate placed against the donor [715]. In 2020, a novel LIFT process, a soft shock-absorbing polydimethylsiloxanecoated transparent support, which the authors called LIFT with OPtical stamp (LIFTOP), was developed to imprint fragile CaPO 4 microchips onto a rigid acceptor substrate.…”

“…The LIFTOP process was initially driven by a laser-induced process such as sacrificial layer ablation to drive the shock-absorbing layer. It was developed to enable the transfer of material from the optical stamp to the target substrate [715]. The description of LIFTOP deposition seems complicated, so let me quote the authors: "As an optical stamp, we used a transparent polyethylene terephthalate (PET) substrate coated with a polydimethylsiloxane (PDMS) shock-absorbing layer.…”

(Bio)manufactured Solutions for Treatment of Bone Defects with an Emphasis on US‐FDA Regulatory Science Perspective

“…[236] In a recent study, functionalization technique, Narazaki et al proposed a novel LIFT method using an optically transparent PDMS-coated stamp for rapid and accurate bioprinting of fibronectin-immobilized CaP on human dentin. [237] Microvalve Bioprinting: Another 3D bioprinting method is microvalve bioprinting, which by definition includes a microvalve in the printhead to control the dripping of bioink droplets, by being at closed and opened states. [238] The fluid motive force is a constant pneumatic pressure.…”

Applications of scanning electron microscopy and focused ion beam milling in dental research

“…While many of these studies elucidate surface morphology, FIB cross-sectioning with SEM and energy dispersive X-ray spectroscopy is the best way to obtain subsurface structural and chemical information. Several publications have demonstrated the use of SEM/FIB cross-sectioning for subsurface imaging of dentin and enamel for studies on morphology [41,[56][57][58][59], effect of treatments [60][61][62][63][64][65][66][67][68][69][70][71][72][73], effect of erosion [74], interface studies [56,[75][76][77][78], and wear mechanisms [3,37,39,40,79,80].…”