Laser Induced Fluorescence Studies of Blood Plasma and Tumor Tissue of Men with Prostate Tumors

Abstract

Objectives: Fluorescence spectroscopy which can be used for optical tissue diagnosis of tumor pathology deserves special interest. The purpose of the work was to study blood plasma and tumor tissue of men with different forms of prostate tumors by using laser induced fluorescence. Blood plasma and tumor tissue of the patients with benign hyperplasia of the prostate (BHP), BHP with inflammation, BHP with high grade PIN (BHP with HGPIN) and adenocarcinoma of prostate (CaP) have been studied. Results: In case of blood plasma fluorescence, intensity of the plasma proteins corresponding peak (340-360 nm) was increasing in the following manner: control group → BHP → BHP with HGPIN → CaP. The intensity of the nicotinamide coenzymes correspond peak (440-460 nm) was increased in case of BHP with HGPIN and CaP patients, but decreased in case of BHP, compared to control. In case of tumor tissue, the changes of the collagen peak (390-400 nm) intensity have been revealed in all cases of prostate tumor tissues. These alterations point to altered collagen biosynthesis levels in different tumor tissues, that reflects the structural changes and characteristics of malignant transformation. Also the changes of the nicotinamide coenzymes peak (440-460 nm) intensity in all spectra of tumor tissues were observed. The highest intensity of the peak was observed in the spectra of BHP with HGPIN and in prostate cancer tissue. Conclusions: Alterations of the coenzymes peak intensities perfectly reflect and are in accordance with the specific energy metabolism of prostate epithelial cells. Normalization of fluorescent spectra from different forms of prostate tumor tissues has shown that, each form has typical spectral shape and ratio of fluorescence peaks intensities. L. Ramishvili et al.

Autofluorescence of blood and its application in biomedical and clinical research

“…A total of 420 nm excitation was found to be better than 405 nm to detect leukoplakia and oral squamous cell carcinoma from normal controls. ES of plasma obtained with LIFS shown changes in fluorescence of plasma proteins (340–360 nm) and reduced nicotinamide coenzymes (440–460 nm), considered as disease driven metabolic changes, and was used to detect prostate tumors (Ramishvili et al, 2014). DL decay kinetics was also used to distinguish leukemic serum samples from healthy controls, showing higher initial intensity, peak weight and lower peak decay rate of DL from leukemic serum than that of the healthy group (Bai et al, 2009; Chen et al, 2012).…”

The diagnostic capability of laser induced fluorescence in the characterization of excised breast tissues

“…Recently, some studies introduced spectroscopic techniques for differentiating between normal and malignant tissues [4][5][6]. Laser-induced fluorescence (LIF) is one of the techniques that have been examined for possible use in the detection of malignancy.…”