Laser-induced fluorescence spectroscopy of human colonic mucosa

Kapadia, Cyrus R.; Cutruzzola, Francis W.; O'Brien, Kenneth M.; Stetz, Mark L.; Enriquez, Rosa E.; Deckelbaum, Lawrence I.

doi:10.1016/0016-5085(90)91242-x

Cited by 222 publications

(107 citation statements)

References 22 publications

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“…Such investigations have demonstrated contrast in a variety of tissue types including: between normal tissue and adenomatous (i.e. neoplastic) polyps ex vivo [27][28][29]; between normal tissue and adenomas in vivo [30]; between normal tissue or hyperplastic polyps and adenomas [31]; between hyperplastic polyps and adenomas [32,33]; and between all three of the above tissue groups [34]. Cancers in the upper GI tract (esophagus and stomach) have also been investigated [35][36][37].…”

Section: Introductionmentioning

confidence: 99%

Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

Coda¹,

Thompson²,

Kennedy³

et al. 2014

Biomed. Opt. Express

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Abstract:We present an ex vivo study of temporally and spectrally resolved autofluorescence in a total of 47 endoscopic excision biopsy/resection specimens from colon, using pulsed excitation laser sources operating at wavelengths of 375 nm and 435 nm. A paired analysis of normal and neoplastic (adenomatous polyp) tissue specimens obtained from the same patient yielded a significant difference in the mean spectrally averaged autofluorescence lifetime −570 ± 740 ps (p = 0.021, n = 12). We also investigated the fluorescence signature of non-neoplastic polyps (n = 6) and inflammatory bowel disease (n = 4) compared to normal tissue in a small number of specimens. References and links1. R. Lambert, H. Saito, and Y. Saito, "High-resolution endoscopy and early gastrointestinal cancer...dawn in the East," Endoscopy 39(3), 232-237 (2007

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Section: Introductionmentioning

confidence: 99%

Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

Coda¹,

Thompson²,

Kennedy³

et al. 2014

Biomed. Opt. Express

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“…Diagnostic oncology studies indicate that the native fluorescence properties of tissues can be exploited to distinguish the normal from the malignant condition in breast (Alfano et al, 1991;Katz et al, 1996), cervix (Glassman et al, 1992) and colon (Kapadia et al, 1990;Schomacker et al, 1992;Yang et al, 1995).…”

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confidence: 99%

Characterization of native fluorescence from DMBA-treated hamster cheek pouch buccal mucosa for measuring tissue transformation

Vengadesan

Aruna²,

Ganesan³

1998

Br J Cancer

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Summary The steady-state native fluorescence spectra of extracts of normal mucosa as well as of different stages of oral lesion of the 7,12-dimethylbenz (a) anthracene (DMBA)-induced hamster cheek pouch carcinogenesis model have been measured and analysed at 405 nm excitation. The emission spectra were scanned from 430 to 700 nm to characterize the native fluorescence of endogenous porphyrin and other fluorophores under various tissue transformation conditions, such as hyperplasia, papilloma, early invasive carcinoma and welldifferentiated squamous cell carcinoma. Two ratio parameters, R1 = (ldl/l6) and R2 = (15,,160), are introduced to quantify the diagnostic potentiality. The ratio values were found to decrease as the stage of the cancer increases. The suggested critical values for both R1 and R2 for normal mucosa is above three, and the suggested critical value for tissues with lesion is less than three. It was also found that the values for R1 and R2 for well-differentiated squamous cell carcinoma is less than one. The ratio parameter R2 is selected for discrimination between normal mucosa and oral lesions, as the difference in the R2 value between normal and DMBA-treated tissue is higher than that for R1.

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“…A number of spectroscopic approaches utilizing tissue autofluorescence and/or light scattering have led the way in the development of photonic methods for in-vivo characterization of tissue structures [1][2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Imaging of tissue microstructures using a multimodal microscope design

Demos

Lieber

Lin

et al. 2005

IEEE J. Select. Topics Quantum Electron.

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Abstract:We investigate a microscope design that offers high signal sensitivity and hyperspectral imaging capabilities and allows for implementation of various optical imaging approaches while its operational complexity is minimized. This system utilizes long working distance microscope objectives that enable for off-axis illumination of the tissue thereby allowing for excitation at any optical wavelength and nearly eliminating spectral noise from the optical elements. Preliminary studies using human and animal tissues demonstrate the feasibility of this approach for real-time imaging of intact tissue microstructures using autofluorescence and light scattering imaging methods.2

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Laser-induced fluorescence spectroscopy of human colonic mucosa

Cited by 222 publications

References 22 publications

Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

Characterization of native fluorescence from DMBA-treated hamster cheek pouch buccal mucosa for measuring tissue transformation

Imaging of tissue microstructures using a multimodal microscope design

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