Laser-induced fluorescence spectroscopy applied to early diagnosis of citrus Huanglongbing

Ranulfi, Anielle C.; Cardinali, M. C. B.; Kubota, T. M. K.; Freitas-Astúa, Juliana; Ferreira, Ednaldo José; Bellete, Barbara Sayuri; Silva, Maria Fátima das Graças Fernandes da; Boas, Paulino Ribeiro Villas; Magalhães, Aida Bebeachibuli; Milori, Débora Marcondes Bastos Pereira

doi:10.1016/j.biosystemseng.2016.02.010

Cited by 32 publications

(39 citation statements)

References 24 publications

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“…They represent the characteristic emission of chlorophyll fluorescence (ChlF). [23][24][25][37][38][39][40] Figure 4 shows the typical fluorescence spectra obtained with the LIFS-561 system, where the two emission peaks, at 680 nm and 730 nm, are characteristic of chlorophyll fluorescence emission (CHlF). [26][27][28][29]40 Figures 5 and 6 show the score plot for three PCs, PC1 Â PC2 Â PC3, of PCA analysis using the fluorescence spectra (LIFS-405 and LIFS-561, respectively) of leaves from different Pera sweet orange accessions grafted in Rangpur lemon rootstock.…”

Section: Resultsmentioning

confidence: 99%

“…The first system has a diode laser CUBE Model produced by Coherent with excitation at 405 nm and maximum output power of 50 mW at room temperature, and a high sensitivity mini-spectrometer manufactured by Ocean Optics (USB4000) with spectral range of 194-894 nm. 18,19,25,30 It will be addressed as LIFS-405 from now on. Referred to as LIFS-561, the second system comprises a diode laser COMPASS model, also produced by Coherent, but with emission at 561 nm.…”

Section: Leaf Samplesmentioning

confidence: 99%

“…23,24 Their applications in agriculture, biology, and botanics has been published in a numerous of reviews in the last 20 years. 18,[23][24][25][26][27][28][29] Laser-induced fluorescence spectroscopy has an optical configuration which allows non-invasive interaction with the sample. Thus, the tool can be made very compact for the development of field devices and incorporated in agricultural vehicles.…”

Section: Introductionmentioning

confidence: 99%

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Laser-induced Fluorescence Spectroscopy (LIFS) for Discrimination of Genetically Close Sweet Orange Accessions (Citrus sinensis L. Osbeck)

et al. 2016

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Although there is substantial diversity among cultivated sweet oranges genotypes with respect to morphological, physiological, and agronomic traits, very little variation at DNA level has been observed. It is possible that this low DNA molecular variability is due to a narrow genetic basis commonly observed in this citrus group. The most different morphological characters observed were originated through mutations, which are maintained by vegetative propagation. Despite all molecular tools available for discrimination between these different accessions, in general, low polymorphism has been observed in all groups of sweet oranges and they may not be identified by molecular markers. In this context, this paper describes the results obtained by using laser-induced fluorescent spectroscopy (LIFS) as a tool to discriminate sweet orange accessions ( Citrus sinensis L. Osbeck) including common, low acidity, pigmented, and navel orange groups, with very little variation at DNA level. The findings showed that LIFS combined with statistical methods is capable to discriminate different accessions. The basic idea is that citrus leaves have multiple fluorophores and concentration depends on their genetics and metabolism. Thus, we consider that the optical properties of citrus leaves may be different, depending on variety. The results have shown that the developed method, for the best classification rate, reaches an average sensitivity and specificity of 95% and 97.5%, respectively. An interesting application of this study is the development of an economically viable tool for early identification in seedling certification, in citrus breeding programs, in cultivar protection, or in germplasm core collection.

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Section: Resultsmentioning

confidence: 99%

Section: Leaf Samplesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Laser-induced Fluorescence Spectroscopy (LIFS) for Discrimination of Genetically Close Sweet Orange Accessions (Citrus sinensis L. Osbeck)

et al. 2016

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“…Today, chlorophyll fluorescence is widely used in research on the correlation with the physiological state of vegetation. Chlorophyll fluorescence detection has achieved good results at the leaf scale and at the canopy scale and has also been used effectively to monitor vegetation water stress [29,30,42,72,73,93,105,106,107,108,109,110,111], ozone stress [66,112,113], nitrogen stress [3,4,54,114,115,116,117,118], pest stress [119,120], GPP [14,15,16,17,18,19,20,21,22,23,121,122,123,124], heat stress [125], and crop productivity [126]. Despite many experiments designed to clarify the relationship between chlorophyll fluorescence and vegetation stress, the internal mechanisms of this relationship remain to be explored.…”

Section: Current Problems and Discussionmentioning

confidence: 99%

Estimation of Chlorophyll Fluorescence at Different Scales: A Review

Huo

et al. 2019

Sensors

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Measuring chlorophyll fluorescence is a direct and non-destructive way to monitor vegetation. In this paper, the fluorescence retrieval methods from multiple scales, ranging from near the ground to the use of space-borne sensors, are analyzed and summarized in detail. At the leaf-scale, the chlorophyll fluorescence is measured using active and passive technology. Active remote sensing technology uses a fluorimeter to measure the chlorophyll fluorescence, and passive remote sensing technology mainly depends on the sun-induced chlorophyll fluorescence filling in the Fraunhofer lines or oxygen absorptions bands. Based on these retrieval principles, many retrieval methods have been developed, including the radiance-based methods and the reflectance-based methods near the ground, as well as physically and statistically-based methods that make use of satellite data. The advantages and disadvantages of different approaches for sun-induced chlorophyll fluorescence retrieval are compared and the key issues of the current sun-induced chlorophyll fluorescence retrieval algorithms are discussed. Finally, conclusions and key problems are proposed for the future research.

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“…12 Ainda na temática do Greening, Ranulfi e colaboradores utilizaram espectros de fluorescência na categorização de plantas sadias, sintomáticas e assintomáticas de HBL. 13 A técnica LIFI também apresenta excitação em 405 nm e utiliza uma câmera digital comercial.…”

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Diagnóstico de doenças de soja através da emissão de fluorescência das folhas: estudo de caso para Ferrugem asiática e \"Haste verde e retenção foliar\"

Neto¹,

Antonio²

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Agradeço à minha orientadora, Dra. Débora Marcondes Bastos Pereira, que idealizou esse projeto, ofereceu todas as condições para que eu pudesse desenvolve-lo e sempre me apoiou nos contratempos que a vida nos põe a prova, e não foram poucos. Agradeço por toda confiança em mim depositada. Agradeço também por me mostrar uma visão interdisciplinar da ciência, que muito me inspirou e certamente carregarei na sequência da minha trajetória acadêmica. Agradeço às Instituições que estive vinculado nos últimos anos: à USP, pelo programa e pela estrutura, à UNIFAP por possibilitar a continuidade do programa, mesmo depois da aprovação no concurso, e também à EMBRAPA Instrumentação, por toda estrutura laboratorial requerida no projeto. Agradeço às agências de pesquisa e fomento CNPq e FAPESP pelo financiamento dos laboratórios e projetos de pesquisa os quais me inseri durante o doutorado. Agradeço à minha família, em especial aos meus pais David Antonio Sbrissa Junior e Elaine Aparecida Lucatti Sbrissa, que sempre me apoiaram nas minhas escolhas acadêmicas e deram todo suporte para que eu chegasse até aqui. Sem eles nada disso seria possível. Agradeço ao Colegiado de Física da UNIFAP, por permitirem minha liberação das atividades acadêmica no ano de 2018 para finalização do trabalho, etapa crucial para a conclusão do trabalho Agradeço amigos do laboratório e de ciência, Carla, Anielle, Alex, Vitão, Max, Amanda, Ká, Guto Alfredo. Muito obrigado pelo apoio, pelo carinho e pela motivação nos momentos difíceis, e em especial, pelos bandejões obrigatórios, que renderam boas conversas e incontáveis equívocos com sobremesas. Agradeço aos meus amigos e físicos brilhantes Luis Felipe (Nó), Renato (Thomp) e Tiago (Timo), por compartilhamos bons momentos nesses últimos anos, com destaque aos intermináveis CIV's marcados para as 18h que começavam as 22h. Agradeço aos amigos do xadrez Vivaldinho, Filipe Guerra, Felipe El Debs, pelas boas histórias e recordações do mundo enxadrístico, que desde sempre guiou meu universo de escolhas. Agradeço, por fim, e em especial, a minha namorada Ana Lidia Salmazo, por esse ainda curto, porém intenso relacionamento. Agradeço por todas as vezes que me apoiou quando precisei, por todas as vezes que me criticou quanto agi errado, e obrigado por me mostrar que podemos continuamente amadurecer, sem deixar de viver com alegria e amor. "Tudo é uma questão de manter A mente quieta A espinha ereta E o coração tranquilo" Walter Franco RESUMO SBRISSA NETO, D. A. Diagnóstico de doenças de soja através da emissão de fluorescência das folhas: estudo de caso para Ferrugem asiática e "Haste verde e retenção foliar". 2019. 99 p. Tese (Doutorado

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Laser-induced fluorescence spectroscopy applied to early diagnosis of citrus Huanglongbing

Cited by 32 publications

References 24 publications

Laser-induced Fluorescence Spectroscopy (LIFS) for Discrimination of Genetically Close Sweet Orange Accessions (Citrus sinensis L. Osbeck)

Laser-induced Fluorescence Spectroscopy (LIFS) for Discrimination of Genetically Close Sweet Orange Accessions (Citrus sinensis L. Osbeck)

Estimation of Chlorophyll Fluorescence at Different Scales: A Review

Diagnóstico de doenças de soja através da emissão de fluorescência das folhas: estudo de caso para Ferrugem asiática e \"Haste verde e retenção foliar\"

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