Laser-induced fluorescence detection of native-fluorescent analytes in column liquid chromatography, a critical evaluation

New near ultraviolet laser-induced native fluorescence detection coupled to HPLC to analyse residues of oxolinic acid and flumequine: a comparison with conventional xenon flash lamp Nuevo método de análisis de residuos de oxolínico y flumequina utilizando la detección de la fluorescencia nativa inducida por láser acoplada en el ultravioleta cercano acoplada al HPLC: comparación con la lámpara de xenon convencional

“…From this point of view, the insertion of LIF detection in liquid chromatography seems suitable. A previous article (van de Nesse et al, 1995) reviews the aspects that need to be considered when applying lasers instead of conventional lamps in fluorescence detection, such as Rayleigh and Raman scatter from the liquid chromatography eluent, background luminescence and laser light reflected and refracted phenomenons at the flow cell. In any case, we are in agreement with this review, that only a few authors have extensively compared their LIF results with data obtained using a conventional fluorescence detector under the same chromatographic conditions.…”

Analysis of serotonin release from single neuron soma using capillary electrophoresis and laser-induced fluorescence with a pulsed deep-UV NeCu laser

“…Gooijer's research group has undertaken a systematic study of pulsed laser performance, including the XeCl excimer lasers, the frequency-quadrupled Nd:YAG lasers, and the nitrogen laser. These lasers were compared to the conventional CW UV laser [34,35]. They also demonstrated that the 280 nm laser line from a pulsed excimerdye laser was more suitable to analyze substituted naphthalene sulphonates in river water than the 257 nm line from a frequency-doubled CW Ar ion laser, because of the interference with shorter excitation wavelength [36].…”

Post-column reaction detection of biotin in human plasma ultrafiltrate based on laser-induced fluorescence energy transfer in the far-red spectral region

“…There are several advantages of laser-induced fluorescence detection over conventional fluorescence. Since the fluorescence intensity is directly proportional to the intensity of the excitation source, detection sensitivity is increased by the high photon flux provided when lasers are used as excitation sources (Hulshoff and Lingeman, 1985;Van De Nesse et al, 1995). In bioanalysis, low concentration detection is important and detection limits in the picogram per milliliter range are often required for compounds in biological matrices.…”