“…Tsuda and Novotny [89] studied the band-broadening in 50 -195 lm ID columns, reporting better results than they had anticipated, at low retention factors. With the short light path lengths of on-column UV detection, the demands on sample concentrations were high, however, and that focused greater attention on use of the more sensitive fluorescence detector, as demonstrated by Folestad et al [90], Jorgenson and Guthrie [39], and van Berkel et al [91]. Although the practical difficulties of both making and handling extremely small diameter columns are considerable, columns with inner diameters of 10 lm and less were used by Tsuda et al [92], Kreijci et al [93], Folestad and Larsson [94], Simon et al [95], Dohmeier and Jorgenson [96], and Göhlin and Larsson [97].…”