Laser-induced fluorescence detection for conventional and microcolumn liquid chromatography

Folestad, Staffan; Johnson, L.D.; Josefsson, Björn; Galle, Bo

doi:10.1021/ac00243a019

Cited by 124 publications

(39 citation statements)

References 15 publications

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“…Tsuda and Novotny [89] studied the band-broadening in 50 -195 lm ID columns, reporting better results than they had anticipated, at low retention factors. With the short light path lengths of on-column UV detection, the demands on sample concentrations were high, however, and that focused greater attention on use of the more sensitive fluorescence detector, as demonstrated by Folestad et al [90], Jorgenson and Guthrie [39], and van Berkel et al [91]. Although the practical difficulties of both making and handling extremely small diameter columns are considerable, columns with inner diameters of 10 lm and less were used by Tsuda et al [92], Kreijci et al [93], Folestad and Larsson [94], Simon et al [95], Dohmeier and Jorgenson [96], and Göhlin and Larsson [97].…”

Section: The Fate Of the Open Tubular Column In Lcmentioning

confidence: 99%

Capillary columns in liquid chromatography: between conventional columns and microchips

Saito

Jinno

Greibrokk³

2004

J of Separation Science

105

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Liquid chromatography on columns with small internal diameters has been reviewed as the intermediate technique between conventional liquid chromatography and microchip separations. The development of micro column separations in the early years has been described, starting with the papers of Horváth and co-workers and Ishii and co-workers, continuing into the first part of the eighties, then making a leap in time to recent innovations with small-bore columns. Based on internal diameters a classification of the different analytical HPLC columns has been suggested. The advantages of small-bore columns have been discussed, with particular emphasis on the advantage of coupling to concentration sensitive detectors when the sample amount is limited. Open tubular columns are treated as a part of the historic background. The recent developments include a brief look into the current status of monolithic columns, the use of packed nano columns and micro columns with electrospray mass spectrometry, and the potential of two-dimensional comprehensive liquid chromatography. Finally, the coupling of sample preparation to analytical columns and the future applications of the novel technological improvements to the microchip separation methods have been discussed.

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Section: The Fate Of the Open Tubular Column In Lcmentioning

confidence: 99%

Capillary columns in liquid chromatography: between conventional columns and microchips

Saito

Jinno

Greibrokk³

2004

J of Separation Science

105

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“…For columns of 5 and 10-pm i.d., a laser induced fluorescence detection system [25] consisting of an He-Cd laser (Model 4210 NB from Liconix) or an Argon ion laser (Model 2025 from Spectra Physics) was used. The layout of the optical system has been described previously [26].…”

Section: Equipmentmentioning

confidence: 99%

Narrow (5‐50‐μm i.d.) open tubular columns in liquid chromatography using immobilized polymethyloctadecylsiloxane as stationary phase

Göhlin

Larsson

1991

J Microcolumn Separations

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Abstract. This paper presents in detail the preparation of highly efficient, stable open tubular (OT) columns for reversed-phase liquid chromatography. Chromatographic properties of 5-50-pm i.d. fused silica columns (1.5-5 m in length) are described, and details of the coating procedure are discussed. On-column fluorescence detection was used for the model substituted-anthracene test solutes. Gas chromatography was used as a complementary technique for column characterization.The stationary phase studied, immobilized polymethyloctadecylsiloxane, PMSCI8, was chosen because of its more lipophilic character compared to other siloxanes. As expected, a higher retention power was found compared to polydimethylsiloxane coatings.The polymeric stationary phase showed good properties. More than 900,000 plates were achieved in 25 min (k = 0.1) in a 5-pm i.d. fused silica OT column. The stationary phase contribution to band broadening was less than 10% in an 11-pm i.d. column at a flow rate thirty times uOpt (k = 1.3).

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“…22 They used a 120-.um-diam exit nozzle to produce a very fine jet of liquid at the exit of the column. However, the low volumetric flow rates employed in capillary separations limited the performance of the liquid jet detector.…”

Section: B Fluorescence Detectionmentioning

confidence: 99%

Laser-based microchemical analysis

Dovic̀hi

1990

Review of Scientific Instruments

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Microchemical analysis is the determination of the chemical composition of small volume samples, typically smaller than 1 mm3. The spatial coherence of the laser has been exploited by workers in many fields to probe these small volume samples. This review considers three classes of microchemical analyses: detectors for microscale separations, spectroscopic studies of minute volume samples, and laser-based microscopy. In the first case, high-sensitivity laser-based detectors are coupled with high-efficiency separation techniques to produce powerful analytical tools for submicroliter volume samples. In the second case, highly selective measurements are made on small volume samples through use of either immunological reagents or spectroscopically rich techniques. In the third case, high spatial resolution images of solid samples are created by recording a spectroscopic signal as a sample is moved with respect to a tightly focused laser beam. In each case, measurements with uniquely high sensitivity, selectivity, and spatial resolution are made possible by use of a laser beam.

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Laser-induced fluorescence detection for conventional and microcolumn liquid chromatography

Cited by 124 publications

References 15 publications

Capillary columns in liquid chromatography: between conventional columns and microchips

Capillary columns in liquid chromatography: between conventional columns and microchips

Narrow (5‐50‐μm i.d.) open tubular columns in liquid chromatography using immobilized polymethyloctadecylsiloxane as stationary phase

Laser-based microchemical analysis

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