Laser‐Induced Differentiation of Human Adipose‐Derived Stem Cells to Temporomandibular Joint Disc Cells

Karic, Vesna; Chandran, Rahul; Abrahamse, Heidi

doi:10.1002/lsm.23332

Cited by 12 publications

(7 citation statements)

References 49 publications

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“…Moreover, a statistical significance was noted in flow cytometry analyses for fibroblasts at 1-week post-irradiation in B (8.97%) and LB group (16.57%). Similar results were published with 660 nm diode laser with statistically significant results in same experimental groups at 2-weeks post-irradiation [41]. Hence, the current study results also indicate possible application of lasers (940 nm Fig.…”

Section: Discussionsupporting

confidence: 90%

940 nm diode laser induced differentiation of human adipose derived stem cells to temporomandibular joint disc cells

2022

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Background Temporomandibular disorder (TMD) refers to a group of disorders that affect temporomandibular joint (TMJ) and its associated muscles with very limited treatment options. Stem cell research is emerging as one of the promising fields in the treatment of degenerative diseases. The ability of human adipose derived stem cells to differentiate into many cell types is driving special interest in several disease management strategies. Photobiomodulation has enhanced the role of these stem cells through their ability to promote cell proliferation and differentiation. Hence, this study examined the differentiation potential of human adipose derived stem cells (ADSCs) into fibroblasts and chondrocytes using a 940 nm diode laser for possible TMD therapy. Materials and methods ADSCs were cultured at different seeding densities and for different time intervals. After irradiation at 24, 48, 72 h, 1, 2 and 3 weeks, ADSC viability and morphological changes were assessed in groups with and without basic fibroblast growth factor. Additionally, the level of adenosine triphosphate (ATP) in the cells was also recorded. The differentiated fibroblasts and chondrocytes were characterized with flow cytometry and immunofluorescence techniques, at 1- and 2-weeks post-irradiation. Results Increased ATP proliferation and cell viability above 90% were observed in all post-irradiation experimental groups. Post irradiation results from flow cytometry and immunofluorescence at 1- and 2‐weeks confirmed the expression of chondrogenic and fibroblastic cell surface markers. Conclusion This study describes stimulatory techniques utilized to differentiate ADSCs into fibroblastic and chondrogenic phenotypes using diode lasers at 940 nm. The study proposes a new treatment model for patients with degenerative disc diseases of the TMJ. The study will offer new possibilities in tissue engineering and TMJ disc management through photobiomodulation of ADSCs using a 940 nm diode laser.

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Section: Discussionsupporting

confidence: 90%

940 nm diode laser induced differentiation of human adipose derived stem cells to temporomandibular joint disc cells

2022

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“…CD90 and CD105 are overexpressed in carcinoma-associated fibroblasts [ 62 ]. PBM may modulate the differentiation status of cultured gingival fibroblasts, which agrees with recent studies describing that laser treatment may affect cell differentiation in human gingival fibroblasts, periodontal ligament cells, and stem cells [ 49 , 63 , 64 ]. The present study represents the first evidence of mesenchymal markers expression modulation in response to PBM in human primary gingival fibroblasts, which in part can explain the LLLI effects on cell differentiation reported in previous studies.…”

Section: Discussionsupporting

confidence: 89%

Photobiomodulation with Red and Near-Infrared Light Improves Viability and Modulates Expression of Mesenchymal and Apoptotic-Related Markers in Human Gingival Fibroblasts

et al. 2021

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Photobiomodulation (PBM), also called low-level laser treatment (LLLT), has been considered a promising tool in periodontal treatment due to its anti-inflammatory and wound healing properties. However, photobiomodulation’s effectiveness depends on a combination of parameters, such as energy density, the duration and frequency of the irradiation sessions, and wavelength, which has been shown to play a key role in laser-tissue interaction. The objective of the study was to compare the in vitro effects of two different wavelengths—635 nm and 808 nm—on the human primary gingival fibroblasts in terms of viability, oxidative stress, inflammation markers, and specific gene expression during the four treatment sessions at power and energy density widely used in dental practice (100 mW, 4 J/cm2). PBM with both 635 and 808 nm at 4 J/cm2 increased the cell number, modulated extracellular oxidative stress and inflammation markers and decreased the susceptibility of human primary gingival fibroblasts to apoptosis through the downregulation of apoptotic-related genes (P53, CASP9, BAX). Moreover, modulation of mesenchymal markers expression (CD90, CD105) can reflect the possible changes in the differentiation status of irradiated fibroblasts. The most pronounced results were observed following the third irradiation session. They should be considered for the possible optimization of existing low-level laser irradiation protocols used in periodontal therapies.

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“…In another study, Funch et al examined the effect of 660 nm wavelength on the trans‐differentiation of ADSCs to fibroblasts and chondrocytes. Their findings suggested the potential use of using light stimulation at this wavelength for the treatment of temporomandibular disorder 300 …”

Section: Biophysical Factorsmentioning

confidence: 99%

Recent progress in the manipulation of biochemical and biophysical cues for engineering functional tissues

Bakhshandeh

Ranjbar

Abbasi

et al. 2022

Bioengineering & Transla Med

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Tissue engineering (TE) is currently considered a cutting-edge discipline that offers the potential for developing treatments for health conditions that negatively affect the quality of life. This interdisciplinary field typically involves the combination of cells, scaffolds, and appropriate induction factors for the regeneration and repair of damaged tissue. Cell fate decisions, such as survival, proliferation, or differentiation, critically depend on various biochemical and biophysical factors provided by the extracellular environment during developmental, physiological, and pathological processes. Therefore, understanding the mechanisms of action of these factors is critical to accurately mimic the complex architecture of the extracellular environment of living tissues and improve the efficiency of TE approaches. In this review, we recapitulate the effects that biochemical and biophysical induction factors have on various aspects of cell fate. While the role of biochemical factors, such as growth factors, small molecules, extracellular matrix (ECM) components, and cytokines, has been extensively studied in the context of TE applications, it is only recently that we have begun to understand the effects of biophysical signals such as surface topography, mechanical, and electrical signals. These biophysical cues could provide a more robust set of stimuli to manipulate cell signaling pathways during the formation of the engineered tissue.Furthermore, the simultaneous application of different types of signals appears to elicit synergistic responses that are likely to improve functional outcomes, which could help translate results into successful clinical therapies in the future.

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Laser‐Induced Differentiation of Human Adipose‐Derived Stem Cells to Temporomandibular Joint Disc Cells

Cited by 12 publications

References 49 publications

940 nm diode laser induced differentiation of human adipose derived stem cells to temporomandibular joint disc cells

940 nm diode laser induced differentiation of human adipose derived stem cells to temporomandibular joint disc cells

Photobiomodulation with Red and Near-Infrared Light Improves Viability and Modulates Expression of Mesenchymal and Apoptotic-Related Markers in Human Gingival Fibroblasts

Recent progress in the manipulation of biochemical and biophysical cues for engineering functional tissues

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