Laser induced cell fusion in combination with optical tweezers: The laser cell fusion trap

Steubing, Rosemarie Wiegand; Cheng, Steve; Wright, W. H.; Numajiri, Yasuyuki; Berns, Michael W.

doi:10.1002/cyto.990120607

Cited by 188 publications

(86 citation statements)

References 21 publications

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“…Steubing et al (1991) pioneered bringing two cells into contact in an optical trap and then fusing them by cutting the common wall of the two cells with several pulses of UV laser microbeams. This method provides an effective way of generating hybrid cells without losing cell functions and has been used to selectively transport Review.…”

Section: Laser Scissors/scalpels and Opto-injectionmentioning

confidence: 99%

Optical tweezers for single cells

Zhang

Liu

2008

J. R. Soc. Interface.

661

441

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Optical tweezers (OT) have emerged as an essential tool for manipulating single biological cells and performing sophisticated biophysical/biomechanical characterizations. Distinct advantages of using tweezers for these characterizations include non-contact force for cell manipulation, force resolution as accurate as 100 aN and amiability to liquid medium environments. Their wide range of applications, such as transporting foreign materials into single cells, delivering cells to specific locations and sorting cells in microfluidic systems, are reviewed in this article. Recent developments of OT for nanomechanical characterization of various biological cells are discussed in terms of both their theoretical and experimental advancements. The future trends of employing OT in single cells, especially in stem cell delivery, tissue engineering and regenerative medicine, are prospected. More importantly, current limitations and future challenges of OT for these new paradigms are also highlighted in this review.

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Section: Laser Scissors/scalpels and Opto-injectionmentioning

confidence: 99%

Optical tweezers for single cells

Zhang

Liu

2008

J. R. Soc. Interface.

661

441

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“…Fertilization is the consequence of egg-sperm fusion, and multinucleated muscle cells are formed from fusion of myoblasts during embryonal development. In vitro, cell-cell fusion can be induced artificially by adding a fusiogenic agent, such as polyethylene glycol (PEG) (1), by subjecting cells in contact with each other to an electric field, which is called electrofusion (1), or by laser-based schemes (2,3).…”

mentioning

confidence: 99%

Manipulating the genetic identity and biochemical surface properties of individual cells with electric-field-induced fusion

Strömberg

Ryttsén

Chiu

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

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“…An important condition for successful fusion-initiated reactions is to stimulate the vesicles in order to obtain an effective fusion event since vesicle fusion is inefficient in the absence of external stimuli. Fusion can be induced artificially by means of membrane stress (Cohen et al, 1982;Shillcock et al, 2005), ions or organic reagents (Estes et al, 2006;Neil et al, 1993;Lentz, 2007;Haluska et al, 2006;Kunishima et al, 2006), DNA (Stengel et al, 2007), proteins (Jahn et al, 2006;Peters et al, 1999 and summary by Walde et al, 2001), laser beams (Steubing et al, 1991;Weber et al, 1992;Kulin et al, 2003) or electric fields (Strömberg et al, 2000). The last one is termed electrofusion.…”

Section: Electrofusion Protocolmentioning

confidence: 99%