Laser-induced breakdown spectroscopy (LIBS) as a novel technique for detecting bacterial infection in insects

Killiny, Nabil; Etxeberria, Ed; Flores, Alejandro Ponce; Blanco, Pedro González; Flores, T.; Ponce, L.

doi:10.1038/s41598-019-39164-8

Cited by 18 publications

(7 citation statements)

References 41 publications

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“…'Todo del Ano' grafted on C. paradisi cv. 'Duncan' rootstock) infected with HLB, where the Zn content tended to increase in lemon varieties and decrease in others citrus [40]. These results are contradictory with those presented in other investigations, and they may have their origin in the physiological changes caused by HLB or the coexistence of Phytophthora [41].…”

Section: Mineral Content Of Citrus Limon and Citrus Sinensiscontrasting

confidence: 59%

Rootstock Influence on Growth and Mineral Content of Citrus limon and Citrus sinensis cv. Valencia Inoculated with Candidatus Liberibacter Asiaticus

et al. 2020

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Huanglongbing (HLB) reduces the growth and development of citrus and induces changes in secondary metabolites such as flavonoids, limonoids, and polyamines. Likewise, infected plants have a deficient absorption of nutrients such as zinc, potassium, manganese, and copper. Therefore, the objective of this study was to evaluate the influence of different rootstocks on morphology and mineral changes of Citrus limon and Citrus sinensis cv. Valencia plants inoculated with Candidatus Liberibacter asiaticus. In a greenhouse of the Experimental Station-Autonomous University of Tamaulipas, the Candidatus Liberibacter asiaticus bacteria were inoculated to Citrus limon plants (growing on Citrus volkameriana, Citrus macrophylla, and Citrus aurantium rootstocks) and Citrus sinensis cv. Valencia (growing on Citrus volkameriana and Citrus aurantium rootstocks). The experiment was established under a completely randomized design with 45 graft/rootstock repetitions. In each graft/rootstock combination, the plant height and stem diameter were determined using a tape measurer and a Vernier, respectively. In addition, the nutrient content of foliar samples was determined by an X-ray fluorescence spectrometer. In both citrus species, the C. aurantium rootstock promoted a higher concentration of the bacteria. On the other hand, the rootstock that showed the best agronomical results after inoculation with the bacteria was C. volkameriana, presenting the least variation in mineral content and conferring greater plant height (15%) and stem diameter (23%). In contrast, the presence of Ca. Liberibacter asiaticus decreased S content and increased Cu concentration in C. lemon plants. Similarly, plants infected with C. sinensis presented higher Fe content. Finally, in both species, no significant differences were observed for Mn, P, and Zn concentration.

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Section: Mineral Content Of Citrus Limon and Citrus Sinensiscontrasting

confidence: 59%

Rootstock Influence on Growth and Mineral Content of Citrus limon and Citrus sinensis cv. Valencia Inoculated with Candidatus Liberibacter Asiaticus

et al. 2020

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“…In the experiment, air was irradiated by high-energy lasers in LIBS and subsequently turned into a plasma state. With all the elements identified, it allowed the spectrometer to properly determine the material's composition [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Analysis and Dynamic Monitoring of Indoor Air Quality Based on Laser-Induced Breakdown Spectroscopy and Machine Learning

et al. 2022

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The air quality of the living area influences human health to a certain extent. Therefore, it is particularly important to detect the quality of indoor air. However, traditional detection methods mainly depend on chemical analysis, which has long been criticized for its high time cost. In this research, a rapid air detection method for the indoor environment using laser-induced breakdown spectroscopy (LIBS) and machine learning was proposed. Four common scenes were simulated, including burning carbon, burning incense, spraying perfume and hot shower which often led to indoor air quality changes. Two steps of spectral measurements and algorithm analysis were used in the experiment. Moreover, the proposed method was found to be effective in distinguishing different kinds of aerosols and presenting sensitivity to the air compositions. In this paper, the signal was isolated by the forest, so the singular values were filtered out. Meanwhile, the spectra of different scenarios were analyzed via the principal component analysis (PCA), and the air environment was classified by K-Nearest Neighbor (KNN) algorithm with an accuracy of 99.2%. Moreover, based on the establishment of a high-precision quantitative detection model, a back propagation (BP) neural network was introduced to improve the robustness and accuracy of indoor environment. The results show that by taking this method, the dynamic prediction of elements concentration can be realized, and its recognition accuracy is 96.5%.

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“…Over the last decades, laser-driven sound and ultrasound generation has attracted interest in a variety of scientific fields and applications, such as laser-induced breakdown spectroscopy 7 , 8 (LIBS), inertial nuclear fusion 9 , control of meteorological phenomena 10 , 11 , military and defense applications 12 , 13 and others. Laser-driven sound generation takes place when short or ultrashort laser pulses of sufficient energy are focused into a gaseous, liquid or solid target.…”

Section: Introductionmentioning

confidence: 99%

Laser-sound: optoacoustic transduction from digital audio streams

Kaleris

Stelzner

Hatziantoniou

et al. 2021

Sci Rep

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This work presents a novel laser-based optoacoustic transducer capable of reproducing controlled and continuous sound of arbitrary complexity in the air or on solid targets. Light-to-sound transduction is achieved via laser-induced breakdown, leading to the formation of plasma acoustic sources in any desired spatial location. The acoustic signal is encoded into pulse streams via a discrete-time audio modulation and is reproduced by fast consecutive excitation of the target medium with appropriately modulated laser pulses. This results in the signal being directly reconstructed at the desired location of the target medium without the need for a receiver or demodulation device. In this work, the principles and evaluation results of such a novel laser-sound prototype system are presented. The performance of the prototype is evaluated by systematic experimental measurements of audio test signals, from which the basic acoustical response is derived. Moreover, a generic computational model is presented that allows for the simulation of laser-sound reproduction of 1-bit or multibit audio streams. The model evaluations are validated by comparison with the acoustic measurements, whereby a good agreement is found. Finally, the computational model is used to simulate an ideal optoacoustic transducer based on the specifications of state-of-the-art commercially available lasers.

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Laser-induced breakdown spectroscopy (LIBS) as a novel technique for detecting bacterial infection in insects

Cited by 18 publications

References 41 publications

Rootstock Influence on Growth and Mineral Content of Citrus limon and Citrus sinensis cv. Valencia Inoculated with Candidatus Liberibacter Asiaticus

Rootstock Influence on Growth and Mineral Content of Citrus limon and Citrus sinensis cv. Valencia Inoculated with Candidatus Liberibacter Asiaticus

Analysis and Dynamic Monitoring of Indoor Air Quality Based on Laser-Induced Breakdown Spectroscopy and Machine Learning

Laser-sound: optoacoustic transduction from digital audio streams

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