Laser Immunotherapy: A Novel Treatment Modality for Metastatic Tumors

Chen, Wei R.; Carubelli, R.; Liu, Hong; Nordquist, Robert E.

doi:10.1385/mb:25:1:37

Cited by 61 publications

(66 citation statements)

References 11 publications

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“…There have been several reports on the generation of antitumour immunity in mouse or rat cancer models by PDT (Korbelik and Cecic, 1999;Korbelik and Dougherty, 1999) and also by a related photothermal technology (Chen et al, 2003). It is thought that tumour-specific cytotoxic T lymphocytes can be generated by these therapies and that these account for the longterm memory immune response against the tumour (Korbelik et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

A green fluorescent protein-expressing murine tumour but not its wild-type counterpart is cured by photodynamic therapy

2006

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The ideal cancer treatment should both destroy the primary tumour and at the same time educate the immune system to recognise the tumour as foreign so that distant metastases will also be eradicated. Photodynamic therapy (PDT) involves the i.v. administration of photosensitisers followed by illumination of the tumour with red light producing reactive oxygen species that eventually cause vascular shutdown and tumour cell death by apoptosis and necrosis. Anti-tumour immunity is stimulated after PDT due to the acute inflammatory response, generation of tumour-specific antigens, and induction of heat-shock proteins. Green fluorescent protein (GFP) is used as an optical reporter to noninvasively image the progression of mouse tumours, and in addition, may act as a foreign (jellyfish) antigen. We asked whether GFP-expressing tumours could be used to monitor the response of tumour-bearing mice to PDT, and whether the tumour response differed when a nonimmunogenic tumour cell line was transduced with GFP. We injected RIF-1 or RIF-1 EGFP (stably transduced with a retroviral vector) cells in the leg of C3H/HeN mice and both the cells and tumour grew equally well. We used PDT with benzoporphyrin derivative and a short drug-light interval. There were complete cures and 100% mouse survival of RIF-1 EGFP while RIF-1 wild-type tumours all recurred. Cured mice were resistant to rechallenge with RIF-1 EGFP cells and a rechallenge with wild-type RIF-1 cells grew significantly slower. There was also slower RIF-1 EGFP rechallenge growth but no rejection when RIF-1 EGFP tumours were surgically removed. There was a low rate of PDT cure of tumours when RIF-1 cells were transduced with an empty retroviral vector. The presence of antibodies against EGFP in mouse serum suggests EGFP can act as a foreign antigen and PDT can then stimulate a long-term memory immune response.

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Section: Discussionmentioning

confidence: 99%

A green fluorescent protein-expressing murine tumour but not its wild-type counterpart is cured by photodynamic therapy

2006

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“…4 4 mg SWNTs were placed in a 50 mL test tube. Then, 8 mL of 0.5% GC was dispensed in the test tube.…”

Section: Immunologically Modi¯ed Carbon Nanotubementioning

confidence: 99%

“…1(a), the SWNTs had a (n, m) ratio distribution of: 23% (6,5), 21% (7,3), 9% (10,3), 9% (7,6), 8% (8,4), and 32% (other). The average diameter from°uorescence analysis of the SWNTs was 0.856 nm [ Fig.…”

Section: Size Distribution Of Swnts and Optical Absorbancementioning

confidence: 99%

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Anti-tumor response induced by immunologically modified carbon nanotubes and laser irradiation using rat mammary tumor model

Acquaviva

Bahavar

Zhou

et al. 2015

J. Innov. Opt. Health Sci.

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The ideal treatment modality for metastatic cancer would be a local treatment that can destroy primary tumors while inducing an e®ective systemic anti-tumor response. To this end, we developed laser immunotherapy, combining photothermal laser application with an immunoadjuvant for the treatment of metastatic cancer. Additionally, to enhance the selective photothermal e®ect, we integrated light-absorbing nanomaterials into this innovative treatment. Speci¯cally, we developed an immunologically modi¯ed carbon nanotube combining single-walled carbon nanotubes (SWNTs) with the immunoadjuvant glycated chitosan (GC). To determine the effectiveness of laser irradiation, a series of experiments were performed using two di®erent irradiation durations -5 and 10 min. Rats were inoculated with DMBA-4 cancer cells, a metastatic cancer cell line. The treatment group of rats receiving laser irradiation for 10 min had a 50% longterm survival rate without residual primary or metastatic tumors. The treatment group of rats receiving laser irradiation for 5 min had no long-term survivors; all rats died with multiple metastases at several distant sites. Therefore, LaserþSWNT-GC treatment with 10 min of laser ¶ Corresponding author. This is an Open Access article published by World Scienti¯c Publishing Company. It is distributed under the terms of the Creative Commons Attribution 3.0 (CC-BY) License. Further distribution of this work is permitted, provided the original work is properly cited. Vol. 8, No. 4 (2015) 1550036 (8 pages irradiation proved to be e®ective at reducing tumor size and inducing long-term anti-tumor immunity. Journal of Innovative Optical Health Sciences

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“…A new compound, glycated chitosan (GC), derived from chitosan by attaching galactose molecules to the chitosan molecules, was developed as a novel immunostimulant. 1,4 GC, as a water soluble compound, is more suitable for in vitro and in vivo biomedical applications. 5,6 LIT using dye-enhanced thermal interaction and GC has been proven to be highly e®ective in the treatment of metastatic tumors in animal studies and pre-clinical studies.…”

Section: Introductionmentioning

confidence: 99%

Anti-Tumor Responses Induced by Laser Irradiation and Immunological Stimulation Using a Mouse Mammary Tumor Model

Zhou

Song

et al. 2013

J. Innov. Opt. Health Sci.

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Anti-tumor immunological response induced by local intervention is ideal for treatment of metastatic tumors. Laser immunotherapy was developed to synergize photothermal interaction with immunological stimulation for cancer treatment. Using an infrared laser, indocyanine green (ICG, as a light absorbing agent), and glycated chitosan (GC, as an immunostimulant), laser immunotherapy has resulted in tumor suppression and anti-tumor responses in pre-clinical as well as clinical studies. To further understand the mechanism of laser immunotherapy, the effects of laser and GC treatment without specific enhancement of laser absorption were studied. Passive adoptive immunity transfer was performed using splenocytes as immune cells. Spleen cells harvested from tumor-bearing mice treated by laser + GC provided 60% immunity in naive recipients. Furthermore, cytotoxicity and TNF-α secretion by splenocytes from treated mice also indicated that laser + G induced immunity was tumor-specific. The high level of infiltrating T cells in tumors after laser + GC treatment further confirmed a specific anti-tumor immune response. Therefore, laser + GC could prove to be a promising selective local treatment modality that induces a systemic anti-tumor response, with appropriate laser parameters and GC doses.

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Laser Immunotherapy: A Novel Treatment Modality for Metastatic Tumors

Cited by 61 publications

References 11 publications

A green fluorescent protein-expressing murine tumour but not its wild-type counterpart is cured by photodynamic therapy

A green fluorescent protein-expressing murine tumour but not its wild-type counterpart is cured by photodynamic therapy

Anti-tumor response induced by immunologically modified carbon nanotubes and laser irradiation using rat mammary tumor model

Anti-Tumor Responses Induced by Laser Irradiation and Immunological Stimulation Using a Mouse Mammary Tumor Model

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