Laser guidance–based cell detection in a microfluidic biochip

Wan, Qin; Schmidt, Lucas; Yang, Xiaoqi; Wei, Lian-Qiang; Huang, Ting; Yuan, Julie X.; Peng, Xiang; Yuan, Xiaocong; Gao, Bruce Z.

doi:10.1117/1.jbo.18.6.060502

Cited by 3 publications

(1 citation statement)

References 10 publications

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“…The microfluidic device was fabricated with multistep process (Fig. 3(c)) using polydimethylsiloxane (PDMS)-based soft lithographic technique similar to the fabrication process reported by previous literatures [29,30]. Based on the physical dimensions of the flow channel in the fabricated microfluidic device, we numerically simulated flow velocity distribution through the flow channel by changing the outlet pumping rates.…”

Section: Capillary Flow Phantommentioning

confidence: 99%

Cerebral capillary velocimetry based on temporal OCT speckle contrast

Choi

Wan

et al. 2016

Biomed. Opt. Express

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Abstract:We propose a new optical coherence tomography (OCT) based method to measure red blood cell (RBC) velocities of single capillaries in the cortex of rodent brain. This OCT capillary velocimetry exploits quantitative laser speckle contrast analysis to estimate speckle decorrelation rate from the measured temporal OCT speckle signals, which is related to microcirculatory flow velocity. We hypothesize that OCT signal due to sub-surface capillary flow can be treated as the speckle signal in the single scattering regime and thus its time scale of speckle fluctuations can be subjected to single scattering laser speckle contrast analysis to derive characteristic decorrelation time. To validate this hypothesis, OCT measurements are conducted on a single capillary flow phantom operating at preset velocities, in which M-mode B-frames are acquired using a high-speed OCT system. Analysis is then performed on the time-varying OCT signals extracted at the capillary flow, exhibiting a typical inverse relationship between the estimated decorrelation time and absolute RBC velocity, which is then used to deduce the capillary velocities. We apply the method to in vivo measurements of mouse brain, demonstrating that the proposed approach provides additional useful information in the quantitative assessment of capillary hemodynamics, complementary to that of OCT angiography.

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Section: Capillary Flow Phantommentioning

confidence: 99%

Cerebral capillary velocimetry based on temporal OCT speckle contrast

Choi

Wan

et al. 2016

Biomed. Opt. Express

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Development of a Compartmentalized Biochip for Axonal Isolation and Neuronal-Circuit Formation at the Single-Cell Level

et al. 2015

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Hydrogel based 3D carriers in the application of stem cell therapy by direct injection

Luan

Liu

Chen

et al. 2017

Nanotechnology Reviews

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Compared with systematic administration such as peripheral intravenous infusion, stem cell therapy by direct injection is theoretically more effective, but some technical barriers such as low stem cell retention rate and low engraftment rate still need to be overcome before its application in humans. Stem cell therapy supported by hydrogel carriers has been increasingly studied in recent years. These hydrogels with properties similar to natural tissues are able to fabricate various forms of carriers, which include in situ forming hydrogels, ex situ forming hydrogels, surface immobilization carriers, microencapsules, and microgels. Some of them are 3D carriers and promise to overcome the technical barriers of stem cell therapy by direct injection. They have different characteristics, application, and prospect in the application of stem cell therapy by direct injection, which is summarized by this review.

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Laser guidance–based cell detection in a microfluidic biochip

Cited by 3 publications

References 10 publications

Cerebral capillary velocimetry based on temporal OCT speckle contrast

Cerebral capillary velocimetry based on temporal OCT speckle contrast

Development of a Compartmentalized Biochip for Axonal Isolation and Neuronal-Circuit Formation at the Single-Cell Level

Hydrogel based 3D carriers in the application of stem cell therapy by direct injection

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