Laser fluorimetry for capillary column liquid chromatography: high-sensitivity detection of derivatized biological compounds

Gluckman, Jennifer C.; Shelly, Dennis C.; Novotný, Miloš V.

doi:10.1016/s0021-9673(01)91684-x

Cited by 75 publications

(32 citation statements)

References 47 publications

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“…Some interesting work -applying laser-induced fluorescence detection in microcolumn LC -has been published by Novotny and co-workers [164,165] and by McGuffin and Zare [166]. McGuffin and Zare [166] detected and analyzed carboxylic acids -after derivatization with 4-bromomethyl-7-methoxycoumarin to match the emission wavelength of a He-Cd laser (325 nm)-in saponified peanut and sesame oils.…”

Section: Fluorescence Detectionmentioning

confidence: 99%

“…The compounds were identified on retention time. An almost similar tag was used by Gluckman et al [165] for the analysis of bile acids and solvolyzed plasma steroids. A steroid standardcontaining nine standard bile acids of approximately 50 pg of each acid -was separated on a capillary LC column, of which a chromatogram is shown in Fig.…”

Section: Fluorescence Detectionmentioning

confidence: 99%

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Microcolumn liquid chromatography: instrumentation, detection and applications

Vissers¹,

Claessens

Cramers

1997

Journal of Chromatography A

208

101

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This review discusses many different aspects of microcolumn liquid chromatography (LC) and reflects the areas of microcolumn LC research interest over the past decades. A brief theoretical discussion on a number of major issues, such as column characterisation, chromatographic dilution effects and extracolumn band broadening in microcolumn LC is given. Recent progress in column technology and the demands and developments of instrumentation and accessories for microcolumn LC are also reviewed. Besides that, the developments in a large number of established and also more recent detection techniques for microcolumn LC are also discussed. The potential of hyphenation of microcolumn LC with other techniques, more particularly of multidimensional chromatography and microcolumn LC coupled to mass spectrometry is reviewed. Finally, the perspective of microcolumn LC separation methods is stressed by a number of relevant applications.

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Section: Fluorescence Detectionmentioning

confidence: 99%

Section: Fluorescence Detectionmentioning

confidence: 99%

Microcolumn liquid chromatography: instrumentation, detection and applications

Vissers¹,

Claessens

Cramers

1997

Journal of Chromatography A

208

101

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“…As an example of the state-of-the-art biochemically important application from the 1980s, we demonstrate in Fig. 3 a chromatogram of solvolyzed plasma steroids on a packed capillary column [55]. The hydroxysteroids were fluorescently labeled to ensure their detection through the laser-induced fluorescence.…”

Section: Evolution Of Molecular Profiling Techniquesmentioning

confidence: 99%

Biochemical individuality reflected in chromatographic, electrophoretic and mass-spectrometric profiles

Novotný

Soini

Mechref

2008

Journal of Chromatography B

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This review discusses the current trends in molecular profiling for the emerging systems biology applications. Historically, the methodological developments in separation science were coincident with the availability of new ionization techniques in mass spectrometry. Coupling miniaturized separation techniques with technologically-advanced MS instrumentation and the modern data processing capabilities are at the heart of current platforms for proteomics, glycomics and metabolomics. These are being featured here by the examples from quantitative proteomics, glycan mapping and metabolomic profiling of physiological fluids.

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“…Novotny (18) has pointed out an alternate trend in the effort to utilize the potential power of the laser for HPLC detection, namely the "tuning" of the chemistry of the system to match the available laser wavelengths (19). The use of naphthalenedialdehyde as discussed above is a good example.…”

Section: Unfortunately Several Aspects Of Laser Light Sources Limit mentioning

confidence: 99%

Detection Limits for Amino Acids in Environmental Samples

Hare

John²

1987

ACS Symposium Series

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Liquid chromatography techniques using fluorescent derivatives and laser-induced fluorescence can separate and detect sub-femtomole levels of amino acids. The widespread distribution of amino acids and proteins from living organisms produces nanomolar and higher levels of amino acid material in most environments of the earth's surface. Contamination usually occurs to some extent during sample collecting and processing and must be recognized and addressed before meaningful amino acid concentrations and distributions can be obtained from environmental samples. Amino acids are distributed ubiquitously throughout much of the earth's crust, including the atmosphereTheir occurrence and important role in living organisms are well known, but amino acids have also been found in fossils and rocks hundreds of millions of years and even billions of years old (2). Amino acids have been reported at parts-per-billion levels in extra-terrestrial samples such as the Apollo moon rocks as well as in several meteorites (3,4). Even distilled water, reagent-grade HC1 and other chemicals frequently contain trace amounts of amino acids. Figure 1 summarizes the levels of amino acids found in several samples of environmental interest.Current analytical techniques for the analysis of the common amino acids are able to detect less than femtomole (10~15) amounts using liquid chromatographic methods with fluorescent derivatives. However, this level of sensitivity may be extremely difficult to utilize because of the widespread presence of amino acids from living organisms. For example, the relatively high levels of amino acids in human body tissues and fluids make human fingerprints or even a person's breath a potential serious contaminant in detecting amino acids in specific environmental samples (5). Sample collecting and preparation are major concerns to amino acid trace analysis. Even with careful handling during sample collection and preparation it is always possible that the sample may have been contaminated in situ before its collection.

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Laser fluorimetry for capillary column liquid chromatography: high-sensitivity detection of derivatized biological compounds

Cited by 75 publications

References 47 publications

Microcolumn liquid chromatography: instrumentation, detection and applications

Microcolumn liquid chromatography: instrumentation, detection and applications

Biochemical individuality reflected in chromatographic, electrophoretic and mass-spectrometric profiles

Detection Limits for Amino Acids in Environmental Samples

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