Laser fluorescence spectroscopy and factor analysis in diagnostics of microbial diseases

Alexandrov, M. T.; Kuzmin, G. P.; Gaponenko, O. G.; Khomenko, V. A.; Khokhlov, E. M.; Tikhonevich, O. V.; Vasiliev, E. N.

doi:10.1134/s1054660x07030061

Cited by 2 publications

(1 citation statement)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fluorescence spectroscopy and microscopy are widely used for different biomedical applications [9,10]. The kindling phenomenon of KFP was successfully used for the development of novel subdiffraction method of fluorescent microscopy (RESOLFT) with the resolution up to 50 -100 nm [11].…”

Section: Introductionmentioning

confidence: 99%

The origin of radiationless conversion of the excited state in the kindling fluorescent protein (KFP): femtosecond studies and quantum modeling

et al. 2011

View full text Add to dashboard Cite

The Ala143Gly variant of the chromoprotein asCP from the sea anemony Anemonia sulcata, called the kindling fluorescent protein (KFP), is a promising candidate for the development of novel subdiffraction method of fluorescent microscopy. The pump-probe method with the delay times between the pump and probe pulses up to 5 ps was applied to study dynamics of the primary processes upon excitation of KFP. The differential absorption spectra at 80 fs delay showed the absorption peak in the range 450-510 nm with the maximum wavelength at 490 nm, which diminished almost twice by intensity by 400 fs and practically disappeared by 1.5 ps. The quantum calculations showed that upon photo-excitation of KFP to the first excited state S1, the fast radiationless relaxation occurred to the ground state S 0 due to rotation of the phenolic fragment of the chromophore. Pump-probe kinetics of KFP excited at 560 nm by a 25 fs pulse. The decay of absorption at 490 nm can be fitted by an exponential function with τ (ΔA560) = 395±15 fs

show abstract

Section: Introductionmentioning

confidence: 99%