Laser doppler spectroscopy: Applications to cell and particle electrophoresis

Covalently Bound Antibody on Polystyrene Latex Beads: Formation, Stability, and Use in Analyses of White Blood Cell Populations

“…Multiangle Doppler electrophoretic light scattering (27) measurements on a Coulter DELSA 440 of suspensions of CD4 and CD8 antibody conjugated beads were used to establish the identity and pH stability range of coated beads layer-by-layer on the original aldehyde/sulfate polystyrene latex beads by virtue of the change in charge that accompanies the application of each successive layer (+DAP + sulfo-SMCC + IT-CD4 or IT-CD8) on the original beads. A rectangular sample channel of 1.00-mm height was positioned at the stationary layer (0.16 mm) near the top of the channel.…”

Dispositif de microélectrophorèse utilisant un vélocimètre optique original à réseau de transmission : aspects théoriques et méthodologiques

“…La vélocimétrie Dopplerlaser, dont il existe différentes variantes [14], a été utilisée sous champ électrique en veine liquide initialement par Ware et Flygare [15,16], et Uzgiris [17,18] ; depuis de nombreux groupes ont développé et adapté cette méthode en fonction des différents types d'échantillons étudiés [19][20][21][22][23][24][25][26][27]. Une mise au point sur la méthode et ses applications a été publiée par Uzgiris [28,29], et Ware et Haas [30].…”

Electrophoretic Light Scattering

“…Therefore, measurements can be performed at any location in the capillary if the scheme to acquire the ACF or to perform direct spectrum analysis can accompany rapid changing field polarity. However, when using a high frequency field, spectrum resolution is inevitably reduced and harmonic sidebands will be produced due to field modulation and this will complicate the spectrum, especially for materials of polydisperse mobility (see Figure 6.17 at the end of this subsection) [53]. [53,54] Another type of ELS cell is square cuvettes, made of plastic or quartz.…”

“…Different formulas have been derived to interpret the spectra having imbedded sideband [53,54]. These sidebands carry no useful information but will overlap and mess up the whole spectrum for multiple-mobility samples.…”

“…A few excellent articles published in the early 1980s summarized the applications up to then [28,53,54]. As of today, there has not been an extensive review of advancements in this field.…”