Laser Direct‐Write Onto Live Tissues: A Novel Model for Studying Cancer Cell Migration

Burks, Hope E.; Phamduy, Theresa B.; Azimi, Mohammad S.; Saksena, Jayant; Burow, Matthew E.; Collins‐Burow, Bridgette M.; Chrisey, Douglas B.; Murfee, Walter L.

doi:10.1002/jcp.25363

Cited by 34 publications

(18 citation statements)

References 53 publications

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“…and allowed optical follow-up. With our method, in vivo studies can be performed with similar results quality and a more relevant animal model 47 .…”

Section: Discussionmentioning

confidence: 99%

Magnetic Resonance Imaging for tracking cellular patterns obtained by Laser-Assisted Bioprinting

Kérourédan

Ribot

Fricain

et al. 2018

Sci Rep

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Recent advances in the field of Tissue Engineering allowed to control the three-dimensional organization of engineered constructs. Cell pattern imaging and in vivo follow-up remain a major hurdle in in situ bioprinting onto deep tissues. Magnetic Resonance Imaging (MRI) associated with Micron-sized superParamagnetic Iron Oxide (MPIO) particles constitutes a non-invasive method for tracking cells in vivo. To date, no studies have utilized Cellular MRI as a tool to follow cell patterns obtained via bioprinting technologies. Laser-Assisted Bioprinting (LAB) has been increasingly recognized as a new and exciting addition to the bioprinting’s arsenal, due to its rapidity, precision and ability to print viable cells. This non-contact technology has been successfully used in recent in vivo applications. The aim of this study was to assess the methodology of tracking MPIO-labeled stem cells using MRI after organizing them by Laser-Assisted Bioprinting. Optimal MPIO concentrations for tracking bioprinted cells were determined. Accuracy of printed patterns was compared using MRI and confocal microscopy. Cell densities within the patterns and MRI signals were correlated. MRI enabled to detect cell patterns after in situ bioprinting onto a mouse calvarial defect. Results demonstrate that MRI combined with MPIO cell labeling is a valuable technique to track bioprinted cells in vitro and in animal models.

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“…and allowed optical follow-up. With our method, in vivo studies can be performed with similar results quality and a more relevant animal model 47 .…”

Section: Discussionmentioning

confidence: 99%

Magnetic Resonance Imaging for tracking cellular patterns obtained by Laser-Assisted Bioprinting

Kérourédan

Ribot

Fricain

et al. 2018

Sci Rep

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“…Laser-assisted bioprinting (LAB) is initially introduced as stereolithography and is similar to inkjet-based bioprinting (IBB) since LAB uses a less viscous bioink to create the spheroids or to directly write onto the tissue (Burks et al, 2016 ). The broad label of LAB can be further defined by laser-guided direct writing (Odde and Renn, 1999 , 2000 ) or modified laser-induced forward transfer (Ringeisen et al, 2002 , 2004 ).…”

Section: D Bioprintingmentioning

confidence: 99%

“…The thin slices of mesenteric tissue facilitate viewing with confocal microscopy. After 5 days of culture, mesentery sections where MDA-MB-231 were printed showed increased number of new capillary sprouts in comparison to mesentery sections without any printing (Phamduy et al, 2015 ; Burks et al, 2016 ). Vascularization during tumor progression was also modeled in a hydrogel composed of an internal collagen/colorectal cancer cell core and an external stromal cover composed of laminin, fibroblast, and endothelial cells.…”

Section: Introductionmentioning

confidence: 99%

Engineering Breast Cancer Microenvironments and 3D Bioprinting

Belgodere

King

Bursavich

et al. 2018

Front. Bioeng. Biotechnol.

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The extracellular matrix (ECM) is a critical cue to direct tumorigenesis and metastasis. Although two-dimensional (2D) culture models have been widely employed to understand breast cancer microenvironments over the past several decades, the 2D models still exhibit limited success. Overwhelming evidence supports that three dimensional (3D), physiologically relevant culture models are required to better understand cancer progression and develop more effective treatment. Such platforms should include cancer-specific architectures, relevant physicochemical signals, stromal–cancer cell interactions, immune components, vascular components, and cell-ECM interactions found in patient tumors. This review briefly summarizes how cancer microenvironments (stromal component, cell-ECM interactions, and molecular modulators) are defined and what emerging technologies (perfusable scaffold, tumor stiffness, supporting cells within tumors and complex patterning) can be utilized to better mimic native-like breast cancer microenvironments. Furthermore, this review emphasizes biophysical properties that differ between primary tumor ECM and tissue sites of metastatic lesions with a focus on matrix modulation of cancer stem cells, providing a rationale for investigation of underexplored ECM proteins that could alter patient prognosis. To engineer breast cancer microenvironments, we categorized technologies into two groups: (1) biochemical factors modulating breast cancer cell-ECM interactions and (2) 3D bioprinting methods and its applications to model breast cancer microenvironments. Biochemical factors include matrix-associated proteins, soluble factors, ECMs, and synthetic biomaterials. For the application of 3D bioprinting, we discuss the transition of 2D patterning to 3D scaffolding with various bioprinting technologies to implement biophysical cues to model breast cancer microenvironments.

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“…41,50 Additionally, recent studies have begun to explore LDW of cancer cells within intact microvascular networks 73 or onto living tissues. 74 These novel techniques provide unique insight into cancer cell dynamics and migration during angiogenesis 73 or within living tissue, 74 and lay the framework to bioprint complex stem cell constructs for regenerative medicine applications. LDW provides a bioprinting modality with excellent print resolution and nozzle-free deposition.…”

Section: Inkjet Bioprintingmentioning

confidence: 99%

“…LDW can also be used in a layer‐by‐layer approach to generate thick, multilayered tissue constructs . Additionally, recent studies have begun to explore LDW of cancer cells within intact microvascular networks or onto living tissues . These novel techniques provide unique insight into cancer cell dynamics and migration during angiogenesis or within living tissue, and lay the framework to bioprint complex stem cell constructs for regenerative medicine applications.…”

Section: Bioprinting Modalities In Regenerative Medicinementioning

confidence: 99%

Stem cell bioprinting for applications in regenerative medicine

Tricomi

Dias

Corr

2016

Annals of the New York Academy of Sciences

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Many regenerative medicine applications seek to harness the biologic power of stem cells in architecturally complex scaffolds or microenvironments. Traditional tissue engineering methods cannot create such intricate structures, nor can they precisely control cellular position or spatial distribution. These limitations have spurred advances in the field of bioprinting, aimed to satisfy these structural and compositional demands. Bioprinting can be defined as the programmed deposition of cells or other biologics, often with accompanying biomaterials. In this concise review, we focus on recent advances in stem cell bioprinting, including performance, utility, and applications in regenerative medicine. More specifically, this review explores the capability of bioprinting to direct stem cell fate, engineer tissue(s), and create functional vascular networks. Furthermore, the unique challenges and concerns related to bioprinting living stem cells, such as viability and maintaining multi- or pluripotency, are discussed. The regenerative capacity of stem cells, when combined with the structural/compositional control afforded by bioprinting, provides a unique and powerful tool to address the complex demands of tissue engineering and regenerative medicine applications.

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Laser Direct‐Write Onto Live Tissues: A Novel Model for Studying Cancer Cell Migration

Cited by 34 publications

References 53 publications

Magnetic Resonance Imaging for tracking cellular patterns obtained by Laser-Assisted Bioprinting

Magnetic Resonance Imaging for tracking cellular patterns obtained by Laser-Assisted Bioprinting

Engineering Breast Cancer Microenvironments and 3D Bioprinting

Stem cell bioprinting for applications in regenerative medicine

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