Laser Desorption Mass Spectrometry. A Review

Hillenkamp, Franz

doi:10.1007/978-3-642-82724-2_125

Cited by 13 publications

(3 citation statements)

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“…The ejection occurs when a volume of the sample material has achieved a critical momentum density. The existence of thresholds for neutral and ion ejection, observed also experimentally [38][39][40][41]1011, has been predicted on the basis of the pressure-pulse model in MALDI context.…”

Section: Particle-induced Desorption Mass Spectrometry Imentioning

confidence: 98%

Particle‐induced desorption in mass spectrometry. Part I. Mechanisms and processes

Demirev

1995

Mass Spectrometry Reviews

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Desorption of (predominantly) organic molecules, induced by particles with keV to MeV energy incident on a condensed phase target, is the topic of this review. The focus is on MeV atomic ion‐induced desorption–plasma desorption (PD). Parallels with keV particle desorption–secondary ion mass spectrometry (SIMS) and fast atom bombardment (FAB)–will be presented more schematically and chiefly for comparison purposes. Current understanding of desorption mechanisms by referring to insights from theory of the processes is surveyed, and the underlying similarities as well as differences between keV and MeV particle desorption are addressed. Several connections between particle‐induced desorption and photon‐induced desorption (e.g., matrix‐assisted laser desorption/ionization‐MALDI) are briefly outlined. Results from computer simulations are used to visualize the dynamics of the desorption process. A brief description of experimental characteristics of desorbed species, aimed at elucidating the mechanisms of desorption, is given, partly to illustrate the use of mass spectrometry in fundamental studies of ion–solid interactions. Ionization pathways in PD and molecular SIMS are briejy summarized. No special emphasis is placed in describing the “generic” instrumental setup f o r particle desorption and only specific technical aspects, not treated elsewhere, eg., time and spatial correlation of the ejected species, are discussed here. In the second part of this review (subsequent article) desorption induced by polyatomic projectiles, and effects, accompanying ion ejection, namely secondary electron emission, impact‐induced photon emission, and sputtering of clusters (fullerenes in particular), are also reviewed. In conclusion, selected examples for analytical applications of desorption methods that may illustrate current and emerging trends and perspectives are discussed. © 1996 John Wiley & Sons, Inc.

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Section: Particle-induced Desorption Mass Spectrometry Imentioning

confidence: 98%

Particle‐induced desorption in mass spectrometry. Part I. Mechanisms and processes

Demirev

1995

Mass Spectrometry Reviews

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“…This was followed by a breakthrough for large molecule laser desorption/ionisation in 1987, when Tanaka et al [2] managed to ionise proteins with a molecular mass of up to 34,000 Da using fine metal powder matrix dispersed in glycerol and a pulsed N 2 laser at 337 nm. There were further reports from the Hillenkamp group demonstrating MALDI on larger peptides and the use of a matrix for pulsed laser desorption MS [3][4][5].…”

Section: Matrix-assisted Laser Desorption Ionisation (Maldi) Mass Spe...mentioning

confidence: 99%

Matrix-assisted laser desorption ionisation (MALDI) mass spectrometry (MS): basics and clinical applications

Israr

Bernieh

Salzano

et al. 2020

Clinical Chemistry and Laboratory Medicine (CCLM)

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AbstractBackgroundMatrix-assisted laser desorption ionisation (MALDI) mass spectrometry (MS) has been used for more than 30 years. Compared with other analytical techniques, it offers ease of use, high throughput, robustness, cost-effectiveness, rapid analysis and sensitivity. As advantages, current clinical techniques (e.g. immunoassays) are unable to directly measure the biomarker; rather, they measure secondary signals. MALDI-MS has been extensively researched for clinical applications, and it is set for a breakthrough as a routine tool for clinical diagnostics.ContentThis review reports on the principles of MALDI-MS and discusses current clinical applications and the future clinical prospects for MALDI-MS. Furthermore, the review assesses the limitations currently experienced in clinical assays, the advantages and the impact of MALDI-MS to transform clinical laboratories.SummaryMALDI-MS is widely used in clinical microbiology for the screening of microbial isolates; however, there is scope to apply MALDI-MS in the diagnosis, prognosis, therapeutic drug monitoring and biopsy imaging in many diseases.OutlookThere is considerable potential for MALDI-MS in clinic as a tool for screening, profiling and imaging because of its high sensitivity and specificity over alternative techniques.

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“…Laser desorption is one of the so called "soft ionization techniques", which means, that especially thermally labile involatile bioorganic molecules can be desorbed and ionized without large fragmentation [3,4]. The field of laser desorption of isolated organic ____ -.…”

Section: Applications In Biologymentioning

confidence: 99%

Laser mass analysis in biology

Spengler¹,

Karas²,

Bahr³

et al. 1989

Ber Bunsenges Phys Chem

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396B. Spengler et al.: Laser Mass Analysis in Biology arise from the eventual need to increase the chooping frequency to improve the spatial resolution. This will decrease the signal.Considerable improvements to the present experiments can be made when one considers the use of radiation resistant phosphors such as the compound semiconductors [4,9, lo]. We are presently pursuing this line of investigation.We feel that LEM should be able to compete well with IR microscopy, since this latter technique, although chemically selective, is limited to a resolution somewhat larger than the operating wavelength, confocal and other recent o p t i d configurations notwithstanding. The LEM should be superior to spontaneous Raman microscopy because of the sample heating problem for most materials with spontaneous Raman. The sophistication necessary in the design of the optical train is considerably simplified as well.Compared to our earlier work on coherent Raman microscopy, namely the CARS microscope [ll], the present technique shouid be able to eventually achieve considerably improved spatial resolution, probably be easier to implement in a turnkey operation, but requires vacuum over the sample and more extensive sample preparation than needed for the CARS microscope.The LEM should be able to perform analyses which can not be done using well known electron beam microprobe or microimaging methods such as X-ray fluorescence, SIMS, Auger, PES, etc. These conventional methods tend to be strongest in providing atomic species, not molecular information. While some of them do give some molecular specificity, they are incapable of the spectral resolution of a laser based method. In principle, if one sought to image a species such as the extremely narrow bandwidth zero phonon lines of a solid, this should be out possible for the LEM.Even using the simple carbon dioxide laser, there is a large range of applications open to this technique because of the many accidental coincidences in the 9 -11 micron portion of the spectrum [7,8]. These potential applications include: imaging organic residues in wafer production; viewing cured and uncured regions of photopolymers; defects, dislocations, etc. in semiconductors; imaging the distribution of non-ra-dioactive isotopic species; imaging crystalline phases differing only in stoichiometry.The authors wish to thank the following members of the staff of the Naval Research Laboratory: Mr. John Peele for the complete mechanical design, modifications and construction of the instrument: Dr. Claudia Randolph for initially suggesting the manganese difluoride phosphor; and Dr. Mark Seaver for many discussions and suggestions. We also wish to thank Dr. Howard Schlossberg of the Air Force Offce of Scientific Research for providing support and encouragement during various stages of this project.

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Laser Desorption Mass Spectrometry. A Review

Cited by 13 publications

References 36 publications

Particle‐induced desorption in mass spectrometry. Part I. Mechanisms and processes

Particle‐induced desorption in mass spectrometry. Part I. Mechanisms and processes

Matrix-assisted laser desorption ionisation (MALDI) mass spectrometry (MS): basics and clinical applications

Laser mass analysis in biology

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