Laser-deposited silver island films: an investigation of their structure, optical properties and SERS activity

Vogel, E.M.; Kiefer, W.; Deckert, Volker; Zeisel, Dieter

doi:10.1002/(sici)1097-4555(199808)29:8<693::aid-jrs283>3.0.co;2-o

Cited by 35 publications

(45 citation statements)

References 17 publications

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“…Colloidal silver clusters, which are widely used for SERS, were deployed with excitation in the near infrared, 4 as well as using visible light for the analysis of extremely small drug concentrations. 5 An important option is the application of silver island films 6 or electrochemically roughened silver surfaces. 7 It was demonstrated that even nonlinear Raman spectroscopy is strongly influenced by the surface enhancement effect.…”

Section: Introductionmentioning

confidence: 99%

Surface enhanced Raman scattering (SERS)—a quantitative analytical tool?

Sackmann

Materny

2006

J Raman Spectroscopy

141

113

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Raman spectroscopy is a widely used analytical tool capable of providing valuable information about the chemical structure and composition of molecules. In order to detect substances also at a very low concentration levels, Surface Enhanced Raman Scattering (SERS) was introduced. The different amplification mechanisms result in extreme sensitivity, however, a quantitative use of SERS appears to be problematic. Especially, when deploying silver sols as SERS substrates, the reproducibility of the signal intensities for a given substance concentration is questionable. Experimental results of an investigation of this problem for low concentrations of adenine are presented. Comparison with results obtained for different SERS substrates by other authors reveals clearly different dependencies. Only in a very limited concentration range reproducible and therefore quantitatively utilizable data could be obtained.

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Section: Introductionmentioning

confidence: 99%

Surface enhanced Raman scattering (SERS)—a quantitative analytical tool?

Sackmann

Materny

2006

J Raman Spectroscopy

141

113

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“…Among SERS-active substrates, vacuum-evaporated nanostructured metal films are well suited for SERS mechanism studies and have a high potential for applications [14][15][16][17][18][19][20]24,[39][40][41][42][43]. The effect on the metal film due to deposition rate, mass thickness, and thermal annealing were studied in detail previously [18,[40][41][42][43].…”

Section: Introductionmentioning

confidence: 99%

Adaptive silver films for surface‐enhanced Raman spectroscopy of biomolecules

Drachev

Thoreson

Nashine

et al. 2005

J Raman Spectroscopy

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The interaction of biological molecules with a typical substrate for surface-enhanced Raman scattering (SERS) often leads to their structural and functional changes. In this work we describe SERS substrates, called adaptive silver films (ASFs), in which the biomaterial and the substrate act in concert to produce excellent Raman enhancement through local restructuring of the metal surface while at the same time preserving the properties (such as conformational state and binding activity) of the analyte. These adaptive substrates show great promise for SERS spectroscopy of many different types of biomolecules, and we provide several current examples of their use. IntroductionOne of main advantages of Raman scattering as a detection method for molecule sensing is well known. Raman spectra enable fingerprinting of molecules which is of particular interest for bio-applications. Surface enhanced Raman scattering (SERS) provides greater detection sensitivity than conventional Raman spectroscopy [1][2][3], and it is quickly gaining traction in the study of biological molecules adsorbed on a metal surface [4][5][6][7][8][9][10][11][12]. SERS spectroscopy allows for the detection and analysis of minute quantities of analytes because it is possible to obtain high-quality SERS spectra at submonolayer molecular coverage as a result of the large scattering enhancements. SERS has also been shown to be sensitive to molecular orientation and to the distance from the metal surface [13]. Thus, SERS is well-suited for biomolecule studies in which specificity and sensitivity to the conformational state and orientation of the molecule are very important.The SERS enhancement mechanism originates in part from the large local electromagnetic fields caused by resonant surface plasmons that can be optically excited at certain wavelengths for metal particles of different shapes or closely spaced groups of particles [14][15][16][17][18][19][20][21]. For aggregates of interacting particles, which are often structured as fractals, plasmon resonances can be excited in a very broad spectral range [22]. In addition to electromagnetic field enhancement, metal nanostructures and molecules can form charge-transfer complexes that provide further enhancement for SERS [23][24][25][26][27][28][29]. The resulting overall enhancement depends critically on the particle or aggregate nanostructure morphology [22,[30][31][32][33][34][35][36], and it can be as high as 10 5 to 10 8 for

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“…The effect of deposition rate, mass thickness dependence, and thermal annealing were studied in detail. 22,[41][42][43][44] The concepts of enhanced local field in aggregated particle films based on a Maxwell-Garnett approach were applied to SERS in the 1980s. 18,19,[21][22][23] These theories account for particle shape and particle interaction and emphasize that the large local fields can only be the result of large field fluctuations.…”

Section: Introductionmentioning

confidence: 99%

Surface-Enhanced Raman Difference between Human Insulin and Insulin Lispro Detected with Adaptive Nanostructures

et al. 2004

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A protein sensor based on nanostructured adaptive silver films (ASFs) is developed for soft protein adsorption and detection with surface-enhanced Raman scattering (SERS). Under protein deposition, the ASFs modify their local nanostructure so that the conformational state of proteins is preserved and SERS is optimized. The sensor is used to examine differences in Raman spectra of two insulin isomers, human insulin and its analogue insulin lispro. These two insulins differ only in the interchange of two neighboring amino acids; specifically, the propyl-lysyl sequence at the C-terminus of the B-chain in insulin lispro is inverted as compared to human insulin. This switch leads to conformational changes at the C-and N-termini and has an important clinical effect for diabetes treatment. The difference in SERS spectra for the two insulins was detected at a submonolayer density, 80 fmol/mm 2 , with only 25 amol in the probed area, and macroscopic enhancement factor 3 × 10 6 . The performed studies indicate that SERS detection using ASFs can lead to efficient proteomic sensing technology.

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Laser-deposited silver island films: an investigation of their structure, optical properties and SERS activity

Cited by 35 publications

References 17 publications

Surface enhanced Raman scattering (SERS)—a quantitative analytical tool?

Surface enhanced Raman scattering (SERS)—a quantitative analytical tool?

Adaptive silver films for surface‐enhanced Raman spectroscopy of biomolecules

Surface-Enhanced Raman Difference between Human Insulin and Insulin Lispro Detected with Adaptive Nanostructures

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