Laser Correlation Spectroscopy of Macromolecular Complexes in Blood Serum as an Effective Method of Monitoring the Progress of Bronchial Asthma in Children

Piruzyan, L. A.; Ковалев, И. Е.; Kovaleva, V. L.; Tyumentseva, E S; Балаболкин, И.И.; Karganov, M. Yu.; Kovaleva, O. I.; Rumyantseva, E. I.

doi:10.1023/b:dobi.0000025560.07930.7d

Cited by 5 publications

(4 citation statements)

References 1 publication

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“…LCS allows measuring particle size in native biological fluids. It is successfully used in clinical practice for evaluation of the disease severity and treatment efficiency in patients with asthma, diabetes mellitus, myasthenia, some haematological diseases, and other pathologies [5,6,[7][8][9][10].…”

Section: Subfraction Composition Of Blood Serum -Laser Correlation Spmentioning

confidence: 99%

The Complex of Tests for the Quantitative Evaluation of the Effects of Radiation on Laboratory Animals

Алчинова¹

2015

AJLS

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Exposure to ionizing radiation leads to exhaustion of the pool of stem cells, increases differentiated cell stress, and activated reparation and apoptosis processes. Apart from well-documented acute effects, radiotherapy leads to delayed effects manifesting in few years after successful treatment. The aim of our study was the development of a test battery for evaluation of irradiation aftereffects in organisms characterized by different radiosensitivity. We chose C57BL mice as the ideal strain, because according to published data, they are characterized by medium radiosensitivity. At the preliminary stage, we have to assess informative value of some methods for the sublethal irradiation dose of 750 R. For evaluation of the damaging effects of radiation (lymphocyte count and maturity), we used, along with classical criteria, some complex parameters (body weight, serum homeostasis, behavior) characterizing general status of the organism. We also performed histological analysis of the liver, spleen, and pancreas and ranged the injuries by their severity.

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Section: Subfraction Composition Of Blood Serum -Laser Correlation Spmentioning

confidence: 99%

The Complex of Tests for the Quantitative Evaluation of the Effects of Radiation on Laboratory Animals

Алчинова¹

2015

AJLS

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“…However, the use of dynamic light scattering methods and solution of the inverse problem by using regularization approach allowed us to construct the particle size distribution from the spectrum of fluctuation of the light scattered on these particles. The range of this method is from nanometers to tens and even hundreds of micrometers (5-6 orders of magnitude), which is unachievable for other methods [11,12]. The obtained results suggest that the method of laser correlation spectroscopy is an adequate and convenient tool for the study of interaction of nanoobjects with components of biological fluids.…”

Section: Figure 5 Lc-histograms Of Blood Serum From Patients With Mmentioning

confidence: 96%

Interaction of Titanium Dioxide Nanoparticles and Blood Serum of Patients with Bronchial Asthma and Myasthenia Gravis

Arkhipova¹

2015

AJCEM

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Abstract:The interest to nanoobjects is mainly attracted by the fact that their properties (and also biological in particular interest to the project) differ drastically from the properties of either bulk material or constituent molecules. The fundamental task is correlation between structure of the nanoparticles and their interaction with surrounding media. The aim of project is a study of interaction of titanium dioxide nanoparticles and blood serum of patients with verified myasthenia and allergy. Laser correlation spectroscopy (LCS) allows fast and easy hydrodynamic diameter determination in a broad range from nanometer to hundred micrometers, which is an advantage in comparison to other technique for a mixture of nano and microobjects of very different sizes, which constitutes biological liquids. LCS measurement of titanium dioxide nanoparticles specimen showed that main part of light scattering is due to particles with radial size 4.64-8.42 nm. LCS and other physical methods measurement data are coincided. Comparison of LC-histograms of blood serum revealed increased contribution of 4.64 -15.28 nm particles into light scattering in bronchial asthma patients and 404.65 -1794.35 nm particles in myasthenia gravis patients. Interaction between titanium dioxide nanoparticles, stabilized by phosphoric acid, and blood serum results to albumin-and globulin-containing complexes formation and precipitation. Interaction between titanium dioxide nanoparticles, stabilized by phosphoric acid, and blood serum results to albumin-and globulin-containing complexes formation and precipitation. Interaction between titanium dioxide nanoparticles and myasthenia patients blood serum results to removing of considerable part (40%) of circulating immune complexes. TiO2 nanoparticles under interaction with asthma patients blood serum form complexes with small size proteins. LCS analysis confirmed removal of particles corresponding by their size to the major serum proteins and redistribution towards larger particles presented by glycolipids, glycoproteins, and antigen-antibody complexes.

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“…This mathematical apparatus for the solution of inverse spectral problem is similar to that used successfully in modern X-ray and NMR tomography. The LCS method was previously applied for evaluation of the disease severity and efficiency of treatment in patients with asthma [12], diabetes mellitus [13,14], myasthenia [15], and other pathologies.…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of the Informative Value of Radioimmunoassay and Laser Correlation Spectroscopy in Myasthenia Gravis

et al. 2014

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The objective of this study was to compare informative value of traditional approach (anti-AChR antibody radioimmunoassay) and evaluation of metabolic shifts by laser correlation spectroscopy in myasthenia gravis. The method based on changes in spectral characteristics of laser radiation caused by scattering in a disperse system yields a histogram reflecting particle contribution into light scatter as a function of particle radius in nanometers. The spectrum of anti-AChR-positive serum is characterized by appreciably increased contribution of particles with a radius 4.6–6.2 nm. Binding of serum components with solubilized AChR confirms that this peak is determined by elevated concentration of antibodies to this receptor. The search for the relationship between the disease severity and the distribution pattern of subfraction serum components revealed three informative zones: 6–15, 27–67, and 127–223 nm. In patients without disturbances of vital functions, the contribution of the first zone particles into light scatter increases and that of the third zone particles decreases. Considerable differences attaining the level of statistical significance in zones 4–6 and 20 nm were revealed in the spectra of serum from patients with myasthenia gravis of the same severity with and without thymoma. This opens prospects for dynamic monitoring of the efficiency of therapy.

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Laser Correlation Spectroscopy of Macromolecular Complexes in Blood Serum as an Effective Method of Monitoring the Progress of Bronchial Asthma in Children

Cited by 5 publications

References 1 publication

The Complex of Tests for the Quantitative Evaluation of the Effects of Radiation on Laboratory Animals

The Complex of Tests for the Quantitative Evaluation of the Effects of Radiation on Laboratory Animals

Interaction of Titanium Dioxide Nanoparticles and Blood Serum of Patients with Bronchial Asthma and Myasthenia Gravis

Comparative Analysis of the Informative Value of Radioimmunoassay and Laser Correlation Spectroscopy in Myasthenia Gravis

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