Laser Correlation Spectroscopy: Nutritional, Ecological and Toxic Aspects

Karganov, M. Yu.; Алчинова, И. Б.; Arkhipova, Ekaterina A.; Skalny, Anatoly V.

doi:10.5772/35254

Cited by 15 publications

(18 citation statements)

References 14 publications

(11 reference statements)

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“…LCS allows measuring particle size in native biological fluids. It is successfully used in clinical practice for evaluation of the disease severity and treatment efficiency in patients with asthma, diabetes mellitus, myasthenia, some haematological diseases, and other pathologies [5,6,[7][8][9][10].…”

Section: Subfraction Composition Of Blood Serum -Laser Correlation Spmentioning

confidence: 99%

The Complex of Tests for the Quantitative Evaluation of the Effects of Radiation on Laboratory Animals

Алчинова¹

2015

AJLS

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Exposure to ionizing radiation leads to exhaustion of the pool of stem cells, increases differentiated cell stress, and activated reparation and apoptosis processes. Apart from well-documented acute effects, radiotherapy leads to delayed effects manifesting in few years after successful treatment. The aim of our study was the development of a test battery for evaluation of irradiation aftereffects in organisms characterized by different radiosensitivity. We chose C57BL mice as the ideal strain, because according to published data, they are characterized by medium radiosensitivity. At the preliminary stage, we have to assess informative value of some methods for the sublethal irradiation dose of 750 R. For evaluation of the damaging effects of radiation (lymphocyte count and maturity), we used, along with classical criteria, some complex parameters (body weight, serum homeostasis, behavior) characterizing general status of the organism. We also performed histological analysis of the liver, spleen, and pancreas and ranged the injuries by their severity.

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Section: Subfraction Composition Of Blood Serum -Laser Correlation Spmentioning

confidence: 99%

The Complex of Tests for the Quantitative Evaluation of the Effects of Radiation on Laboratory Animals

Алчинова¹

2015

AJLS

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“…Laser correlation spectroscopy (LCS) allows analysis of the subfraction composition of bioingredients in a wide range of their molecular sizes (from 1 to 10,000 nm). From the physical point of view, the method is based on principles of Doppler spectroscopy [8]. Detailed LC-histograms formed by the spectrometer operational program consist of 32 columns.…”

Section: Methodsmentioning

confidence: 99%

Metabolic and Morphological Changes in Rats’ Eye after 7-Days Microgravity Simulation

Vyalkina¹,

Fedorov²,

Эскина³

et al. 2015

AJLS

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Microgravity simulation in rats resulted to changes in eye lavage content. Molecular subfraction analysis with laser correlation spectroscopy revealed significant differences in the pattern of particle distribution in five intervals: 1.9 -2.56 nm, 4.64-6.25 nm, 91.3 nm, 165-300 nm, and 400-734 nm. Microgravity modeling induced a series of interrelated processes in the retina of experimental rats manifesting in decreasing of total and layer-by-layer thickness of the retina, mild edema, and consequently, the increasing of specific density of neurons. In the inner nuclear layer, the cell density decreased because of the amacrine and bipolar cells. These changes could be explained by relative ischemia of the retinal layers, which are dependent on the retinal vessels circulation system.

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“…Subfraction composition of the obtained culture medium was analyzed by laser correlation spectroscopy (LCS) [5]. The method allows evaluating the ratio of particles of various sizes in biological fluids (serum, urine, etc.).…”

Section: Methodsmentioning

confidence: 99%

Changes in Serum and Cell Homeostasis under Irradiation: Factors of Distant Action

Алчинова¹

2015

AJLS

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Extracellular DNA-containing substances can be used as a marker of the balance between two processes: elimination of cells with critical number of lesions and repair of genetic material due to the work of adaptive systems. The idea of using this criterion was inspired by the results of measuring exDNA in blood plasma of chronically irradiated individuals. Here we used material obtained after gamma-irradiation (total dose 0.5 Gy) and subsequent culturing of PHA-stimulated human peripheral blood lymphocytes. The culture medium and exDNA isolated by various methods were treated with DNase and pronase. The concentration of free exDNA isolated by phenol extraction was an individual sign. The culture medium supernatant contained considerable amounts of exDNA in the form of nucleoprotein; cleavage of the protein component of this nucleoprotein considerably increased the size of fragments (from 1 to 20 kb) and reduced their resistance to DNase.

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Laser Correlation Spectroscopy: Nutritional, Ecological and Toxic Aspects

Cited by 15 publications

References 14 publications

The Complex of Tests for the Quantitative Evaluation of the Effects of Radiation on Laboratory Animals

The Complex of Tests for the Quantitative Evaluation of the Effects of Radiation on Laboratory Animals

Metabolic and Morphological Changes in Rats’ Eye after 7-Days Microgravity Simulation

Changes in Serum and Cell Homeostasis under Irradiation: Factors of Distant Action

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