Laser Capture Microdissection of Murine Embryonic Neural Crest Cells

Greene, Robert M.; Smolenkova, Irina; Pisano, M. Michele

doi:10.1007/978-1-4939-9412-0_10

Cited by 2 publications

(2 citation statements)

References 26 publications

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“…Laser capture microdissection (LCM) is a microscopicguided system that can isolate specific cell types or regions of interest from tissue sections with a laser beam [21]. A variety of studies have indicated that LCM is powerful for isolating distinct cells and is helpful for the subsequent analysis of DNA, RNA, or protein profiles by sequencing or microarray [22][23][24]. With respect to the intestine, LCM has been used to obtain high-integrity RNA samples from freshly resected human intestinal tissue and to evaluate the gene expression profiles between crypts and villi of ileal epithelial cells [25,26].…”

Section: Introductionmentioning

confidence: 99%

circFLNA promotes intestinal injury during abdominal sepsis through Fas-mediated apoptosis pathway by sponging miR-766-3p

Shi

Zhang

et al. 2023

Inflamm. Res.

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Background Intra-abdominal infections are the second most common cause of sepsis in the intensive care unit. Intestinal epithelial injury due to abdominal sepsis results in a variety of pathological changes, such as intestinal bacteria and toxins entering the blood, leading to persistent systemic inflammation and multiple organ dysfunction. The increased apoptosis of intestinal epithelial cells induced by sepsis further exacerbates the progression of sepsis. Although several studies have revealed that circRNAs are involved in intestinal epithelial injury in sepsis, few studies have identified the roles of circRNAs in intestinal epithelial apoptosis. Methods We used laser capture microdissection to obtain purified epithelial cells located in intestinal crypts from four patients with abdominal sepsis induced by intestinal perforation and four samples from age and sex-matched non-septic patients. Microarray analysis of circRNAs was conducted to assess differentially expressed circRNAs between patients with and without sepsis. Lastly, in vitro and in vivo assays were performed to study the mechanism of circFLNA in intestinal epithelial apoptosis during sepsis. Results circFLNA was upregulated in the intestinal epithelium after abdominal sepsis induced by intestinal perforation. Inhibition of miR-766-3p impaired si-circFLNA-mediated inhibition of apoptosis and inflammation factor levels in lipopolysaccharide (LPS)-treated HIEC-6 cells. circFLNA aggravated apoptosis and inflammation through the Fas-mediated apoptosis pathway in both LPS-treated HIEC-6 cells and a mouse cecal ligation and puncture model. Conclusion Our findings showed that circFLNA promotes intestinal injury in abdominal sepsis through the Fas-mediated apoptosis pathway by sponging miR-766-3p. The circFLNA/miR-766-3p/Fas axis has potential as a novel therapeutic target for treating intestinal injury in sepsis.

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Section: Introductionmentioning

confidence: 99%

circFLNA promotes intestinal injury during abdominal sepsis through Fas-mediated apoptosis pathway by sponging miR-766-3p

Shi

Zhang

et al. 2023

Inflamm. Res.

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“…Within this cadre of technologies, Laser Capture Microdissection (LCM) [ 4 - 6 ] has proven to be a reliable means of procuring tissue cellular populations, achieving high sensitivity and high precision for multiplex genomic and proteomic profiling [ 4 , 7 - 9 ]. LCM has been used to generate data for a wide variety of research questions, ranging from entomology [ 10 - 12 ], agriculture research [ 13 , 14 ], embryology [ 15 , 16 ], metabolic disease [ 17 - 19 ], heart disease [ 20 , 21 ], neurobiology [ 22 , 23 ], infectious disease [ 24 - 26 ], and cancer [ 6 ] (see below).…”

Section: Introductionmentioning

confidence: 99%

Laser Capture Proteomics: spatial tissue molecular profiling from the bench to personalized medicine

Liotta

Pappalardo

Carpino

et al. 2021

Expert Review of Proteomics

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Introduction: Laser Capture Microdissection (LCM) uses a laser to isolate, or capture, specific cells of interest in a complex heterogeneous tissue section, under direct microscopic visualization. Recently, there has been a surge of publications using LCM for tissue spatial molecular profiling relevant to a wide range of research topics. Areas Covered: We summarize the many advances in tissue Laser Capture Proteomics (LCP) using mass spectrometry for discovery, and protein arrays for signal pathway network mapping. This review emphasizes: a) transition of LCM phosphoproteomics from the lab to the clinic for individualized cancer therapy, and b) the emerging frontier of LCM single cell molecular analysis combining proteomics with genomic, and transcriptomic analysis. The search strategy was based on the combination of MeSH terms with expert refinement. Expert Opinion: LCM is complemented by a rich set of instruments, methodology protocols, and analytical A.I. (artificial intelligence) software for basic and translational research. Resolution is advancing to the tissue single cell level. A vision for the future evolution of LCM is presented. Emerging LCM technology is combining digital and AI guided remote imaging with automation, and telepathology, to a achieve multi-omic profiling that was not previously possible.

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Laser Capture Microdissection of Murine Embryonic Neural Crest Cells

Cited by 2 publications

References 26 publications

circFLNA promotes intestinal injury during abdominal sepsis through Fas-mediated apoptosis pathway by sponging miR-766-3p

circFLNA promotes intestinal injury during abdominal sepsis through Fas-mediated apoptosis pathway by sponging miR-766-3p

Laser Capture Proteomics: spatial tissue molecular profiling from the bench to personalized medicine

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