Laser-Based Fluorimetric Detection Schemes for the Analysis of Proteins by Capillary Zone Electrophoresis

Swaile, David F.

doi:10.1080/01483919108049292

Cited by 93 publications

(38 citation statements)

References 35 publications

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“…In 1991, Swaile and Sepaniak [59] were the first to detect the native fluorescence of proteins in CE using a 257 nm excitation wavelength with an argon-ion laser operated at 514 nm with frequency doubling. However, the LOD obtained for conalbumin was poor (0.25 nM).…”

Section: Detection Of Native Fluorescent Peptides and Proteinsmentioning

confidence: 99%

“…On-column, on-capillary or in-capillary derivatization can be a valuable way to avoid complicated set-ups, dilution and turbulence problems encountered with postcolumn derivatization [59]. It can be achieved by three different strategies, named at-inlet, zone-passing and throughoutcapillary techniques, depending on where and how derivatization reaction occurs.…”

Section: Derivatization Modes and Set-upsmentioning

confidence: 99%

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LIF detection of peptides and proteins in CE

2007

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CE- and microchip-based separations coupled with LIF are powerful tools for the separation, detection and determination of biomolecules. CE with certain configurations has the potential to detect a small number of molecules or even a single molecule, thanks to the high spatial coherence of the laser source which permits the excitation of very small sample volumes with high efficiency. This review article discusses the use of LIF detection for the analysis of peptides and proteins in CE. The most common laser sources, basic instrumentation, derivatization modes and set-ups are briefly presented and special attention is paid to the different fluorogenic agents used for pre-, on- and postcapillary derivatization of the functional groups of these compounds. A table summarizing major applications of these derivatization reactions to the analysis of peptides and proteins in CE-LIF and a bibliography with 184 references are provided which covers papers published to the end of 2005.

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Section: Detection Of Native Fluorescent Peptides and Proteinsmentioning

confidence: 99%

Section: Derivatization Modes and Set-upsmentioning

confidence: 99%

LIF detection of peptides and proteins in CE

2007

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“…Swaile and Sepaniak [45] were the first of demonstrate native fluorescence with on-column detection using UV excitation (frequency-doubled Ar-ion laser, 257 nm). They were capable of detection conalbumin at a [47,48].…”

Section: Direct On-column Detectionmentioning

confidence: 99%

Detection in capillary electrophoresis

2000

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“…tryptophan, tyrosine, and phenylalanine) could be directly applied to protein detection with high sensitivity [12][13][14], not all proteins could generate native fluorescence signals. Therefore, the development and application of fluorescence reagents is indispensable to improve the detection sensitivity of proteins [15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%