Laser-based cell printing techniques for additive biomanufacturing

Vinson, Benjamin T.; Sklare, Samuel C.; Chrisey, Douglas B.

doi:10.1016/j.cobme.2017.05.005

Cited by 15 publications

(6 citation statements)

References 81 publications

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“…However, challenges associated with droplet inconsistency, low cell density, easy nozzle blockage, and physical stresses on cells limit the range of this technique’s applicability. Alternatively, laser-based bioprinting also enables droplet-based printing for single cell manipulation or 3D spheroid formation[ 116 , 117 ]. Using the platform, laser direct-write, Kingsley et al .…”

Section: Spheroid Generation Methodsmentioning

confidence: 99%

Using Spheroids as Building Blocks Towards 3D Bioprinting of Tumor Microenvironment

Zhuang¹,

Chiang²,

Fernanda³

et al. 2024

IJB

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Cancer still ranks as a leading cause of mortality worldwide. Although considerable efforts have been dedicated to anticancer therapeutics, progress is still slow, partially due to the absence of robust prediction models. Multicellular tumor spheroids, as a major three-dimensional (3D) culture model exhibiting features of avascular tumors, gained great popularity in pathophysiological studies and high throughput drug screening. However, limited control over cellular and structural organization is still the key challenge in achieving in vivo like tissue microenvironment. 3D bioprinting has made great strides toward tissue/organ mimicry, due to its outstanding spatial control through combining both cells and materials, scalability, and reproducibility. Prospectively, harnessing the power from both 3D bioprinting and multicellular spheroids would likely generate more faithful tumor models and advance our understanding on the mechanism of tumor progression. In this review, the emerging concept on using spheroids as a building block in 3D bioprinting for tumor modeling is illustrated. We begin by describing the context of the tumor microenvironment, followed by an introduction of various methodologies for tumor spheroid formation, with their specific merits and drawbacks. Thereafter, we present an overview of existing 3D printed tumor models using spheroids as a focus. We provide a compilation of the contemporary literature sources and summarize the overall advancements in technology and possibilities of using spheroids as building blocks in 3D printed tissue modeling, with a particular emphasis on tumor models. Future outlooks about the wonderous advancements of integrated 3D spheroidal printing conclude this review.

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Section: Spheroid Generation Methodsmentioning

confidence: 99%

Using Spheroids as Building Blocks Towards 3D Bioprinting of Tumor Microenvironment

Zhuang¹,

Chiang²,

Fernanda³

et al. 2024

IJB

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“…[187] Another interesting binding strategy is the use of DNA-directed immobilization (DDI), as demonstrated by Lemma et al for selective deposition of two different cell types on 2PL written scaffold structures. [188] The direct positioning of cells, approach (II), has been addressed with noncontact printing methods like extrusion/dropon-demand (DoD) printing of cells and spheroids, [190,191] laserbased cell printing, [192] and inkjet based cell printing. [189] In addition, there are also physical methods to position cells.…”

Section: Engineering the Spatial Contact Of Cells With Bioelectronic ...mentioning

confidence: 99%

Printed Electronic Devices and Systems for Interfacing with Single Cells up to Organoids

Saghafi,

Vasantham,

Hussain

et al. 2023

Adv Funct Materials

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The field of bioelectronics with the aim to contact cells, cell clusters, biological tissues and organoids has become a vast enterprise. Currently, it is mainly relying on classical micro‐ and nanofabrication methods to build devices and systems. Very recently the field is highly pushed by the development of novel printable organic, inorganic and biomaterials as well as advanced digital printing technologies such as laser and inkjet printing employed in this endeavor. Recent advantages in alternative additive manufacturing and 3D printing methods enable interesting new routes, in particular for applications requiring the incorporation of delicate biomaterials or creation of 3D scaffold structures that show a high potential for bioelectronics and building of hybrid bio‐/inorganic devices. Here the current state of printed 2D and 3D electronic structures and related lithography techniques for the interfacing of electronic devices with biological systems are reviewed. The focus lies on in vitro applications for interfacing single cell, cell clusters, and organoids. Challenges and future prospects are discussed for all‐printed hybrid bio/electronic systems targeting biomedical research, diagnostics, and health monitoring.

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“…To overcome the limitations, BioLP was developed by Barron et al . utilizing a three-layer approach by including a laser absorption interlayer (thickness: 75 – 100 nm) to prevent the laser direct interaction with the bioink[ 34 ], which improves the reproducibility of the droplet ejection and makes BioLP more efficient than other laser-assisted biomanufacturing techniques.…”

Section: Additive Biomanufacturing Techniquesmentioning

confidence: 99%

Hybrid biomanufacturing systems applied in tissue regeneration

Liu¹,

Aslan

2022

IJB

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Scaffold-based approach is a developed strategy in biomanufacturing, which is based on the use of temporary scaffold that performs as a house of implanted cells for their attachment, proliferation, and differentiation. This strategy strongly depends on both materials and manufacturing processes. However, it is very difficult to meet all the requirements, such as biocompatibility, biodegradability, mechanical strength, and promotion of cell-adhesion, using only single material. At present, no single bioprinting technique can meet the requirements for tissue regeneration of all scales. Thus, multi-material and mixing-material scaffolds have been widely investigated. Challenges in terms of resolution, uniform cell distribution, and tissue formation are still the obstacles in the development of bioprinting technique. Hybrid bioprinting techniques have been developed to print scaffolds with improved properties in both mechanical and biological aspects for broad biomedical engineering applications. In this review, we introduce the basic multi-head bioprinters, semi-hybrid and fully-hybrid biomanufacturing systems, highlighting the modifications, the improved properties and the effect on the complex tissue regeneration applications.

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Laser-based cell printing techniques for additive biomanufacturing

Cited by 15 publications

References 81 publications

Using Spheroids as Building Blocks Towards 3D Bioprinting of Tumor Microenvironment

Using Spheroids as Building Blocks Towards 3D Bioprinting of Tumor Microenvironment

Printed Electronic Devices and Systems for Interfacing with Single Cells up to Organoids

Hybrid biomanufacturing systems applied in tissue regeneration

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