Laser‐Assisted Strain Engineering of Thin Elastomer Films to Form Variable Wavy Substrates for Cell Culture

Tomba, Caterina; Petithory, Tatiana; Pedron, Riccardo; Airoudj, Aissam; Meglio, Ilaria Francesca Di; Roux, Aurélien; Luchnikov, Valériy

doi:10.1002/smll.201900162

Cited by 12 publications

(21 citation statements)

References 54 publications

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“…As previously described 41 , silicone elastomer films (Silex Ltd, Linford, UK, super clear sheet, 0.5mm thick, #HT6240 40SH) were prestressed up to 100% of its length with homemade stretchers (composition: stainless steel and Teflon) and exposed with a laser beam (Gravotech, Rillieux-la-Pape, France, LS100 laser). The same stretchers were used for the experiments of compression and stretching of a flat cell monolayer.…”

Section: Methodsmentioning

confidence: 99%

“…11140035), at 37 °C and 5% CO 2 . MDCK Myr-Palm-GFP H2B-mCherry was generated as previously described 41 . Cell lines were regularly tested negative for contamination with mycoplasma (Eurofins GATC Biotech, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…To combine high-statistics, a time resolution of few minutes after rolling and a high-resolution imaging, cells were chemically fixed with 4% paraformaldehyde (PFA) at different timepoints after rolling. We used MDCK stably expressing the 6 nucleus marker H2B-RFP and the plasma membrane marker Myr-Lyn-GFP 41 or MDCK labelled with the plasma membrane marker CellMask and the nuclear stain Hoechst (see Methods). To analyze their shape, we segmented cells using Limeseg, a 3D cell segmentation ImageJ plugin 42 , and we quantified the average cell volume over time, both on planar and tubular regions ( Fig.…”

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confidence: 99%

“…We thus wondered how the response of the epithelium would be when both curvatures are induced on the same substrate. To do this, we took advantage of a technique that we recently developed to produce wavy substrates 41 . First, a thin hard layer at the surface of a prestrained elastomer is formed by infrared laser irradiation, which then spontaneously adopts a wrinkled morphology upon stress relaxation ( Fig.…”

mentioning

confidence: 99%

“…Wavy substrates. As previously described 41 37 °C and 5% CO2. MDCK Myr-Palm-GFP H2B-mCherry was generated as previously described 41 .…”

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confidence: 99%

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Transient active osmotic swelling of epithelium upon curvature induction

Tomba

Luchnikov

Barberi

et al. 2020

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Generation of tissue curvature is essential to morphogenesis. However, how cells adapt to changing curvature is still unknown because tools to dynamically control curvature in vitro are lacking. Here we developed self-rolling substrates to study how flat epithelial cell monolayers adapt to a rapid, anisotropic change of curvature. We show that the primary response is an active and transient osmotic swelling of cells. This cell volume increase is not observed on inducible wrinkled substrates, where concave and convex regions alternate each other over short distances, identifying swelling as a collective response to changes of curvature with persistent sign over large distances. It is triggered by a drop in membrane tension and actin depolymerization, perceived by cells as a hypertonic shock. Osmotic swelling restores tension while actin reorganizes, probably to comply with curvature. Epithelia are thus unique materials that transiently, actively swell while adapting to large curvature induction.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

“…Wavy substrates. As previously described 41 37 °C and 5% CO2. MDCK Myr-Palm-GFP H2B-mCherry was generated as previously described 41 .…”

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confidence: 99%

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Transient active osmotic swelling of epithelium upon curvature induction

Tomba

Luchnikov

Barberi

et al. 2020

Preprint

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Curvature in Biological Systems: Its Quantification, Emergence, and Implications across the Scales

et al. 2023

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Surface curvature both emerges from, and influences the behavior of, living objects at length scales ranging from cell membranes to single cells to tissues and organs. The relevance of surface curvature in biology has been supported by numerous recent experimental and theoretical investigations in recent years. In this review, we first give a brief introduction to the key ideas of surface curvature in the context of biological systems and discuss the challenges that arise when measuring surface curvature. Giving an overview of the emergence of curvature in biological systems, its significance at different length scales becomes apparent. On the other hand, summarizing current findings also shows that both single cells and entire cell sheets, tissues or organisms respond to curvature by modulating their shape and their migration behavior. Finally, we address the interplay between the distribution of morphogens or micro-organisms and the emergence of curvature across length scales with examples demonstrating these key mechanistic principles of morphogenesis. Overall, this review highlights that curved interfaces are not merely a passive by-product of the chemical, biological and mechanical processes but that curvature acts also as a signal that co-determines these processes.

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Wrinkled Interfaces: Taking Advantage of Anisotropic Wrinkling to Periodically Pattern Polymer Surfaces

et al. 2023

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Periodically patterned surfaces can cause special surface properties and are employed as functional building blocks in many devices, yet remaining challenges in fabrication. Advancements in fabricating structured polymer surfaces for obtaining periodic patterns are accomplished by adopting "top-down" strategies based on self-assembly or physico-chemical growth of atoms, molecules, or particles or "bottom-up" strategies ranging from traditional micromolding (embossing) or micro/nanoimprinting to novel laser-induced periodic surface structure, soft lithography, or direct laser interference patterning among others. Thus, technological advances directly promote higher resolution capabilities. Contrasted with the above techniques requiring highly sophisticated tools, surface instabilities taking advantage of the intrinsic properties of polymers induce surface wrinkling in order to fabricate periodically oriented wrinkled patterns. Such abundant and elaborate patterns are obtained as a result of self-organizing processes that are rather difficult if not impossible to fabricate through conventional patterning techniques. Focusing on oriented wrinkles, this review thoroughly describes the formation mechanisms and fabrication approaches for oriented wrinkles, as well as their fine-tuning in the wavelength, amplitude, and orientation control. Finally, the major applications in which oriented wrinkled interfaces are already in use or may be prospective in the near future are overviewed.

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Laser‐Assisted Strain Engineering of Thin Elastomer Films to Form Variable Wavy Substrates for Cell Culture

Cited by 12 publications

References 54 publications

Transient active osmotic swelling of epithelium upon curvature induction

Transient active osmotic swelling of epithelium upon curvature induction

Curvature in Biological Systems: Its Quantification, Emergence, and Implications across the Scales

Wrinkled Interfaces: Taking Advantage of Anisotropic Wrinkling to Periodically Pattern Polymer Surfaces

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