Laser-assisted skin delivery of immunocontraceptive rabies nanoparticulate vaccine in poloxamer gel

Bansal, Amit; Gamal, Wael; Menon, Ipshita; Olson, Victoria A.; Wu, Xianfu; D’Souza, Martin J.

doi:10.1016/j.ejps.2020.105560

Cited by 13 publications

(3 citation statements)

References 36 publications

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“…Thus, our novel transdermal vaccine has the potential to induce a cell-mediated immune response. This can be attributed to the Langerhans cells present in the skin that take up the antigen and stimulate the immune response by delivering the antigen to nearby lymph nodes [ 15 , 16 , 17 , 18 , 22 , 34 ]. However, in addition to CD4/CD8a expression, a detailed analysis of the cytokine profile is also necessary to supplement the cell-mediated immune response.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, our research group explored transdermal vaccine delivery strategies, such as dissolving microneedles (MNs) [ 15 , 20 ] and LASER ablation-mediated delivery [ 16 , 21 , 22 ], for measles, influenza, COVID-19, and rabies. MNs are micron-sized needles that form microchannels when inserted into the skin, facilitating the entry of vaccine antigens into the skin.…”

Section: Introductionmentioning

confidence: 99%

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Zika Vaccine Microparticles (MPs)-Loaded Dissolving Microneedles (MNs) Elicit a Significant Immune Response in a Pre-Clinical Murine Model

et al. 2023

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Although the global Zika epidemic in 2015–16 fueled vaccine development efforts, there is no approved Zika vaccine or treatment available to date. Current vaccine platforms in clinical trials are administered via either subcutaneous or intramuscular injections, which are painful and decrease compliance. Therefore, in the present study, we explored Zika vaccine microparticles (MPs)-loaded dissolving microneedles (MNs) with adjuvant MPs encapsulating Alhydrogel® and MPL-A® administered via the transdermal route as a pain-free vaccine strategy. We characterized the MNs for needle length, pore formation, and dissolvability when applied to murine skin. Further, we evaluated the in vivo efficacy of vaccine MPs-loaded MNs with or without adjuvants by measuring the immune response after transdermal immunization. The vaccine MPs-loaded dissolving MNs with adjuvants induced significant IgG, IgG1, and IgG2a titers in immunized mice compared to the untreated control group. After the dosing regimen, the animals were challenged with Zika virus, monitored for seven days, and sacrificed to collect spleen and lymph nodes. The lymphocytes and splenocytes from the immunized mice showed significant expressions of helper (CD4) and cytotoxic (CD8a) cell surface markers compared to the control group. Thus, this study puts forth a ‘proof-of-concept’ for a pain-free transdermal vaccine strategy against Zika.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Zika Vaccine Microparticles (MPs)-Loaded Dissolving Microneedles (MNs) Elicit a Significant Immune Response in a Pre-Clinical Murine Model

et al. 2023

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“…In several studies, the effectiveness and suitability of polymeric-based vaccine delivery systems for adjuvant and vaccine delivery applications were examined. The special property of polymeric-based systems is that they serve as adjuvants, may trigger an antigen-mediated immune response, and also substantially deliver the antigen or vaccine to the desired anatomical or physiological location [ 12 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 ]. Moreover, the benefit of polymeric-based delivery methods is that they may be made using natural polymers, which reduces the risk of tissue cytotoxicity [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Natural Polymeric Composites Derived from Animals, Plants, and Microbes for Vaccine Delivery and Adjuvant Applications: A Review

Nordin

Husna

Ahmad

et al. 2023

Gels

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A key element in ensuring successful immunization is the efficient delivery of vaccines. However, poor immunogenicity and adverse inflammatory immunogenic reactions make the establishment of an efficient vaccine delivery method a challenging task. The delivery of vaccines has been performed via a variety of delivery methods, including natural-polymer-based carriers that are relatively biocompatible and have low toxicity. The incorporation of adjuvants or antigens into biomaterial-based immunizations has demonstrated better immune response than formulations that just contain the antigen. This system may enable antigen-mediated immunogenicity and shelter and transport the cargo vaccine or antigen to the appropriate target organ. In this regard, this work reviews the recent applications of natural polymer composites from different sources, such as animals, plants, and microbes, in vaccine delivery systems.

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A recombinant Leishmania amastigote-specific protein, rLiHyG, with adjuvants, protects against infection with Leishmania infantum

Machado¹,

Lage²,

Vale³

et al. 2022

Acta Tropica

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Laser-assisted skin delivery of immunocontraceptive rabies nanoparticulate vaccine in poloxamer gel

Cited by 13 publications

References 36 publications

Zika Vaccine Microparticles (MPs)-Loaded Dissolving Microneedles (MNs) Elicit a Significant Immune Response in a Pre-Clinical Murine Model

Zika Vaccine Microparticles (MPs)-Loaded Dissolving Microneedles (MNs) Elicit a Significant Immune Response in a Pre-Clinical Murine Model

Natural Polymeric Composites Derived from Animals, Plants, and Microbes for Vaccine Delivery and Adjuvant Applications: A Review

A recombinant Leishmania amastigote-specific protein, rLiHyG, with adjuvants, protects against infection with Leishmania infantum

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