Laser assisted bioprinting of engineered tissue with high cell density and microscale organization

Guillotin, Bertrand; Souquet, Agnès; Catros, Sylvain; Duocastella, Martí; Pippenger, Benjamin E.; Bellance, Séverine; Bareille, Reine; Rémy, Murielle; Bordenave, Laurence; Amédée, Joëlle; Guillemot, Fabien

doi:10.1016/j.biomaterials.2010.05.055

Cited by 701 publications

(440 citation statements)

References 38 publications

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“…To image the depth and morphology of injection, 0.2 μm fluorescent beads (Thermo Fischer, FluoSpheres® F8811) are added to the ink with a concentration of 5 × 10 9 beads/ mL. For the experiments investigating the potential of our technique as a direct three-dimensional cell-delivery vehicle, cell-like fluorescent beads (∅ 10 μm, density: 1.05 g mL −1 , Polysciences, Fluoresbrite® YG) were also added to the ink with a concentration of 1 × 10 7 beads/mL, similar to cell concentrations used in previous studies on cell delivery using LIFT [16,22].…”

Section: Lift For Direct Three-dimensional Liquid Deliverymentioning

confidence: 99%

“…In standard LIFT, a thin layer (10-100 μm) of the liquid to deliver is coated onto a transparent donor slide. Upon absorption of a nanosecond laser pulse by an absorptive layer of the donor slide, a shockwave is generated, which produces a liquid microjet towards a receiver slide [14][15][16][17]. Thus, LIFT can directly deliver biologically relevant liquids over the two dimensions of the donor slide without any reloading steps.…”

Section: Introductionmentioning

confidence: 99%

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Depth-controlled laser-induced jet injection for direct three-dimensional liquid delivery

et al. 2018

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Needle-free jet injection enables the delivery of drugs into skin or soft tissue by puncturing them with a high-velocity liquid jet. However, precise and efficient drug delivery requires generating such liquid jets with both a controlled velocity and a high throughput, which remains challenging with current spring-and gas-actuated jet injectors. Here, we propose a depth-controlled and high-throughput injection method by adapting laser-induced forward transfer (LIFT), a high-resolution two-dimensional printing technique, for direct three-dimensional liquid delivery into soft tissues.The velocity of thin liquid jets is laser actuated from 10 to 85 m/s so that doses as small as 10 pL, not achievable with other injectors, are injected at a 1 Hz repetition rate into a 300 μm thick soft gelatin substrate with a 25 μm depth precision and 12 μm lateral resolution. We further investigate the potential of this liquid delivery technique as a direct three-dimensional cell-delivery vehicle and show that depth-controlled particle delivery requires high-delivery efficiency. Our direct three-dimensional liquid delivery system opens up more possibilities for pinpoint drug delivery in soft tissues or tissue-engineered constructs.

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Section: Lift For Direct Three-dimensional Liquid Deliverymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Depth-controlled laser-induced jet injection for direct three-dimensional liquid delivery

et al. 2018

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“…Laser-assisted bioprinting also enables a great control over the droplet size and number of printed cells per droplet via manipulation of the laser pulse energy, the laser spot size, the distance between the ribbon and the substrate, and the thickness of both energy absorbing layer and cell support layer [53,57,59,89]. Several works have also shown the ability of laser-assisted bioprinting to print mammalian cells without affecting their viability or function, or inducing DNA damage [14,53,55,58,60,89,90,200,203].…”

Section: Laser-assisted Bioprintingmentioning

confidence: 99%

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

et al. 2017

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Bioprinting technologies are powerful additive biofabrication techniques to produce cellular constructs for skin tissue engineering owing to their unique ability to precisely pattern living and non-living materials in predefined spatial locations. This unique feature, combined with the computer controlled printing and medical imaging techniques, enable researchers and clinicians to generate patient specific constructs partly replicating the intricate compositional and architectural organization of the skin. Bioprinting has been used to automatically dispense hydrogels with skin cells located in prescribed sites that promote skin formation in vitro and in vivo. Current skin bioprinting approaches mostly rely on the sequential printing of fibroblasts and keratinocytes embedded within a homogeneous hydrogel. Although such approaches have already been translated to pre-clinical scenarios, they still present limitations in terms of fully replicating the cellular and extracellular matrix (ECM) heterogeneity in native skin. The success of bioprinting for skin repair strongly depends on the design of printable bioinks capable of supporting the function of printed cells and stimulating the production of new ECM components. To better mimic the human skin, novel developments in dedicated bioprinting technologies, in the design of bioinks, as well as in the printing of vascularised constructs are necessary. This paper presents an overview regarding the use of bioprinting for skin tissue engineering applications. The operating principles of bioprinting technologies are outlined along with requirements of printed skin constructs. Finally, preclinical results are summarized and future perspectives for the field are highlighted.

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“…There are three main methods of bioprinting, each with their own working ideology: laser, inkjet and extrusion [52,53]. Laser-assisted biological printers are based on the laser-induced forward transfer (LIFT) technique and provide the ability to allocate the material with micrometer resolution [54]. Three things are required for laser bioprinting: a laser pulse source, a biological target ribbon and a substrate that acts as a receiver for the printed material [54].…”

Section: Integrins In Tissue Engineeringmentioning

confidence: 99%

“…Laser-assisted biological printers are based on the laser-induced forward transfer (LIFT) technique and provide the ability to allocate the material with micrometer resolution [54]. Three things are required for laser bioprinting: a laser pulse source, a biological target ribbon and a substrate that acts as a receiver for the printed material [54]. The ribbon is composed of a thin metal layer-usually gold or titanium-that can absorb the material [54].…”

Section: Integrins In Tissue Engineeringmentioning

confidence: 99%

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2017

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Chronic kidney disease (CKD) is the leading cause of death for individuals suffering from end-stage kidney failure. While kidney transplantation is the most viable solution to CKD, it has many challenges and limitations. This review evaluates kidney transplant rejection and the various transplantation methods that may be employed to treat CKD. Kidney rejection and long-term transplant survival is the main concern regarding transplantation attempts. Thus, scientist must screen and strategize appropriately to ensure kidney transplant survival. Tissue engineering techniques are explored as means to improve upon transplantation attempts. Research into ensuring adequate organ scaffold design has become crucial in the field of regenerative medicine. Furthermore, the role of integrins is discussed as a central component for future tissue engineering techniques, by taking the study of scaffold design to the molecular level. Finally, a glimpse into the current advancements of three-dimensional bioprinting highlights the future of tissue engineering and shows how the field of regenerative medicine is taking another step closer to developing "home-grown" kidneys. With the prospect of generating a fully lab-created, personalized kidney, CKD may one day be treated without encountering any of the limitations that have hindered previous attempts.

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Laser assisted bioprinting of engineered tissue with high cell density and microscale organization

Cited by 701 publications

References 38 publications

Depth-controlled laser-induced jet injection for direct three-dimensional liquid delivery

Depth-controlled laser-induced jet injection for direct three-dimensional liquid delivery

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

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