Laser ablation of microdroplets for copper isotopic analysis via MC-ICP-MS. Analysis of serum microsamples for the diagnosis and follow-up treatment of Wilson's disease

Abstract

Cu isotopic analysis -can provide valuable hindsight when investigating Wilson’s disease (WD), but one of the problems related with this type of study is that usually low sample volumes are...

Atomic spectrometry update: review of advances in the analysis of clinical and biological materials, foods and beverages

“…External reproducibility from independently digested and puried CRMs was found to be 0.05& for d 123 Sb. Another interesting piece of work 71 reported total Cu and Cu isotope determination in microvolumes of serum (2 to 15 mL) for the purpose of diagnosis and treatment follow-up in Wilson's disease. The total Cu method involved direct injection of only 1 mL of pre-treated serum followed by time resolved ICP-MS analysis, achieving an LOD of 3 mg L À1 .…”

Cu fractionation, isotopic analysis, and data processing via machine learning: new approaches for the diagnosis and follow up of Wilson's disease via ICP-MS

“…Values in that range (0.932-0.999 mg L −1 ) have also been reported for some WD patients, due to the eventual release of Cu from the liver. 12 In the case of 24 h urinary Cu excretion, the cutoff is set at an excreted Cu amount of higher than 100 mg in 24 h. However, asymptomatic WD patients sometimes show an excreted Cu amount lower than such a threshold (57-95 mg). These are only some examples of the issues preventing efficient WD diagnosis.…”

“…3,13,14 Hence it is important to continue the research on new methods to diagnose WD at an early stage before any symptoms appear. In this regard, investigations focused on Cu isotopic analysis 12,15,16 or on parameters related to the Cu bound to ceruloplasmin and/or free Cu 13,[17][18][19] have been carried out.…”

Isotopic Analysis by Laser Ablation Solution Sampling MC-ICP-MS─An Example of Boron

“…One tends to convert liquids into solids even when LA is chosen as a sample introduction method for solution samples. 5 For conventional solution-based MC-ICP-MS analysis, it is usually necessary to separate analytes from matrix elements (e.g., by chromatography columns) to minimize isobaric/polyatomic interferences and matrix effects. 6 The purification process usually consumes many chemicals and utensils (e.g., ultrapure water, acids, tubes, resin, and columns), poses risks of contamination and isotopic fractionation, and is time-consuming and labor-intensive.…”

“…Besides isotopic measurements in polyphase fluid inclusions, , to the best of our knowledge, currently there is no study on LA direct sampling of solution samples for MC-ICP-MS isotopic analysis. One tends to convert liquids into solids even when LA is chosen as a sample introduction method for solution samples . For conventional solution-based MC-ICP-MS analysis, it is usually necessary to separate analytes from matrix elements (e.g., by chromatography columns) to minimize isobaric/polyatomic interferences and matrix effects .…”