Laryngotracheal reconstruction with porous titanium in rabbits: are vascular carriers and mucosal grafts really necessary?

Janssen, Luuk M.; Osch, Gerjo J. V. M. van; Li, Jia Ping; Kops, Nicole; Groot, K. de; Feenstra, Louw; Hardillo, José A.

doi:10.1002/term.254

Cited by 21 publications

(6 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, recently there have been attempts to use titanium implants in other medical indications, particularly for tracheal replacement. [2][3][4][5] These efforts necessitate a better understanding of the interaction of titanium surfaces with soft tissues. The behaviour of different cell types in contact with titanium can be distinctly different.…”

Section: Introductionmentioning

confidence: 99%

Controlled implant/soft tissue interaction by nanoscale surface modifications of 3D porous titanium implants

et al. 2015

View full text Add to dashboard Cite

Porous titanium implants are widely employed in the orthopaedics field to ensure good bone fixation. Recently, the use of porous titanium implants has also been investigated in artificial larynx development in a clinical setting. Such uses necessitate a better understanding of the interaction of soft tissues with porous titanium structures. Moreover, surface treatments of titanium have been generally evaluated in planar structures, while the porous titanium implants have complex 3 dimensional (3D) architectures. In this study, the determining factors for soft tissue integration of 3D porous titanium implants were investigated as a function of surface treatments via quantification of the interaction of serum proteins and cells with single titanium microbeads (300-500 μm in diameter). Samples were either acid etched or nanostructured by anodization. When the samples are used in 3D configuration (porous titanium discs of 2 mm thickness) in vivo (in subcutis of rats for 2 weeks), a better integration was observed for both anodized and acid etched samples compared to the non-treated implants. If the implants were also pre-treated with rat serum before implantation, the integration was further facilitated. In order to understand the underlying reasons for this effect, human fibroblast cell culture tests under several conditions (directly on beads, beads in suspension, beads encapsulated in gelatin hydrogels) were conducted to mimic the different interactions of cells with Ti implants in vivo. Physical characterization showed that surface treatments increased hydrophilicity, protein adsorption and roughness. Surface treatments also resulted in improved adsorption of serum albumin which in turn facilitated the adsorption of other proteins such as apolipoprotein as quantified by protein sequencing. The cellular response to the beads showed considerable difference with respect to the cell culture configuration. When the titanium microbeads were entrapped in cell-laden gelatin hydrogels, significantly more cells migrated towards the acid etched beads. In conclusion, the nanoscale surface treatment of 3D porous titanium structures can modulate in vivo integration by the accumulative effect of the surface treatment on several physical factors such as protein adsorption, surface hydrophilicity and surface roughness. The improved protein adsorption capacity of the treated implants can be further exploited by a pre-treatment with autologous serum to render the implant surface more bioactive. Titanium microbeads are a good model system to observe these effects in a 3D microenvironment and provide a better representation of cellular responses in 3D.

show abstract

Section: Introductionmentioning

confidence: 99%

Controlled implant/soft tissue interaction by nanoscale surface modifications of 3D porous titanium implants

et al. 2015

View full text Add to dashboard Cite

show abstract

“…We have previously utilized the titanium tracheal implants in mice and sheep with success (Schultz et al, 2002, 2004). Other groups also had success with titanium implants in respiratory area (Janssen et al, 2009, 2010). Trachea is an advantageous area for reconstruction as it is a thin tissue with relatively low oxygen requirements (Kalathur et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Modification of macroporous titanium tracheal implants with biodegradable structures: Tracking in vivo integration for determination of optimal in situ epithelialization conditions

Vrana¹,

Dupret‐Bories²,

Bach³

et al. 2012

Biotech & Bioengineering

View full text Add to dashboard Cite

Previously, we showed that macroporous titanium implants, colonized in vivo together with an epithelial graft, are viable options for tracheal replacement in sheep. To decrease the number of operating steps, biomaterial-based replacements for epithelial graft and intramuscular implantation were developed in the present study. Hybrid microporous PLLA/titanium tracheal implants were designed to decrease initial stenosis and provide a surface for epithelialization. They have been implanted in New Zealand white rabbits as tracheal substitutes and compared to intramuscular implantation samples. Moreover, a basement membrane like coating of the implant surface was also designed by Layer-by-Layer (LbL) method with collagen and alginate. The results showed that the commencement of stenosis can be prevented by the microporous PLLA. For determination of the optimum time point of epithelialization after implantation, HPLC analysis of blood samples, C-reactive protein (CRP), and Chromogranin A (CGA) analyses and histology were carried out. Following 3 weeks the implant would be ready for epithelialization with respect to the amount of tissue integration. Calcein-AM labeled epithelial cell seeding showed that after 3 weeks implant surfaces were suitable for their attachment. CRP readings were steady after an initial rise in the first week. Cross-linked collagen/alginate structures show nanofibrillarity and they form uniform films over the implant surfaces without damaging the microporosity of the PLLA body. Human respiratory epithelial cells proliferated and migrated on these surfaces which provided a better alternative to PLLA film surface. In conclusion, collagen/alginate LbL coated hybrid PLLA/titanium implants are viable options for tracheal replacement, together with in situ epithelialization.

show abstract

“…Recently, development of metallic foams with porosities that would allow in‐growth of cells in vivo has become a very active area of research 4. Utilization of metallic foams enables utilization of titanium in soft tissue applications such as tracheal replacement where mechanical properties of the structure are vital 5. In such structures, understanding the mechanisms of the interactions of the 3D titanium structures with soft tissues is important, but it has not been as widely studied as the interaction of titanium with hard tissues.…”

Section: Introductionmentioning

confidence: 99%

Zucker, Wasser und Neuronen

Lindhorst

2016

Chemie in nserer Zeit

View full text Add to dashboard Cite

Openly porous structures in implants are desirable for better integration with the host tissue. Sintered microbead‐based titanium implants for oto‐rhinolaryngology applications, which create an environment where the cells can migrate in the areas between the microbeads, are developed. This structure promotes fibrovascular tissue formation within the implant in vivo. In this study, it is determine to what extent these events can be controlled by changing the physical environment of the implants both in vitro and in vivo. By cell tracking, it is observed that the size of the beads and the distance between the neighboring beads significantly affect the ability of cells to develop cell‐to‐cell contacts and to bridge the pores. Live cell staining shows that as the bead size gets smaller, the probability to observe cells that fill the porous areas is higher. This also affects the initial attachment and distribution of the cells and collagen secretion by fibroblasts. Obtaining a fast coverage of the system also enables co‐culture systems where, the number and the distribution of the second cell type are boosted by the presence of the first. This concept is utilized to increase the attachment of vascular endothelial cells by an initial layer of fibroblasts. By decreasing the bead diameter, the overall colonization of the implant can be significantly increased in vivo. The effect of bead size has a similar pattern both in rats and rabbits, with faster colonization of smaller bead‐based structures. Using smaller beads would improve clinical outcomes as faster integration facilitates the attainment of functionality by the implant.

show abstract

Laryngotracheal reconstruction with porous titanium in rabbits: are vascular carriers and mucosal grafts really necessary?

Cited by 21 publications

References 19 publications

Controlled implant/soft tissue interaction by nanoscale surface modifications of 3D porous titanium implants

Controlled implant/soft tissue interaction by nanoscale surface modifications of 3D porous titanium implants

Modification of macroporous titanium tracheal implants with biodegradable structures: Tracking in vivo integration for determination of optimal in situ epithelialization conditions

Zucker, Wasser und Neuronen

Contact Info

Product

Resources

About