Larvicidal and histopathology effect of endophytic fungal extracts of Aspergillus tamarii against Aedes aegypti and Culex quinquefasciatus

Baskar, Kannan; Ragavendran, Chinnasamy; Pandy, K. N.; Venkatesan, Manigandan; Sebastian, Prakash Joy; Murugesan, Subban; Thomas, Adelina; Kweka, Eliningaya J.

doi:10.1016/j.heliyon.2020.e05331

Cited by 26 publications

(23 citation statements)

References 79 publications

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“…The ethyl acetate extract of Fusarium equiseti ef-2, an endophytic fungus, had significant larvicidal activity against Culex mosquito larvae. This complements recent findings that used the same ethyl acetate extract of another endophytic fungus, Aspergillus tamarii, against two mosquito larvae, Aedes aegypti and Culex quinquefasciatus 47 . The ethyl acetate extract of Cochliobolus spicifer, an endophyte from the date palm Phoenix dactylifera, larvicidal activity against Aedes caspius and Culex pipiens 48 .…”

Section: Discussionsupporting

confidence: 86%

Endophytic Fungi Fusarium Equiseti EF2 Isolated from Leucas Aspera: A Novel Biocontrol Agent Against Culex Sp

Kavitha

Aarthi²,

Prakash

2022

Int J Life Sci Pharm Res

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Among several life-threatening diseases, vector-borne diseases play a significant role. Mosquito-borne conditions are dangerous and are more prevalent. Culex is the most prevalent and causative agent of many zoonotic diseases among various mosquito species. Endophytic fungi that reside in the healthy plant produce an array of bioactive compounds against several conditions causing pathogens. Thus, this study aimed to identify a novel antilarval combination from the endophytic fungi present in Leucas aspera leaves. The objectives include the isolation of endophytic fungi from leaf samples, selection of potent antilarval endophyte, and characterization of its bioactive compound. The exploration of the least studied fungi, Fusarium equiseti as an endophyte in the leaves of Leucas aspera with potent antilarval properties is an inquisitive discovery. A total of 10 endophytic fungi (EF-1 to EF-10) were isolated and screened for the larvicidal activity of the fungal broth and its spore. The best isolate, EF-2 was identified as Fusarium equiseti. The crude sample and the active fraction of the ethyl acetate extract exhibited potent antilarval properties against Culex mosquito larvae with 90% mortality. Phytochemical analysis and characterization studies by UV-Vis spectroscopy and GC-MS revealed bioactive compounds in the active fraction of the extract. Overall, this study suggested a new option for biocide formulation that could aid in the effort to control mosquitoes. Among several discoveries of bioactive compounds from the plant extracts, this study has identified novel compounds from its endophytic fungi rather than the plant itself. The extracts of endophytic fungi Fusarium equiseti isolated from Leucas aspera, has antilarvicidal activity.

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Section: Discussionsupporting

confidence: 86%

Endophytic Fungi Fusarium Equiseti EF2 Isolated from Leucas Aspera: A Novel Biocontrol Agent Against Culex Sp

Kavitha

Aarthi²,

Prakash

2022

Int J Life Sci Pharm Res

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“…Silosuh et al (Silo-Suh et al, 1994) isolated a strain of Bacillus cereus UW85 that controlled alfalfa damping-off by inhibiting oomycete pathogens and producing two antimicrobial substances, aminopolyols and aminoglycosides. Baskar et al (2020) isolated and identified an endophytic fungus from the cactus Aspergillus tamarii based on the fact that its mycelial metabolite ethyl acetate has a good insecticidal effect. An efficient system for the bioconversion of sucrose to oligofructose (FOS) was obtained using Aspergillus tamarii NKRC 1229 mycelial fructosyltransferase (Choukade and Kango, 2019).…”

Section: Effect Of Inoculation With Growthpromoting Strains On Tobaccomentioning

confidence: 99%

Isolation and identification of symbiotic strains in Paris fargesii Franch that promote disease resistance

Yan

Wang²,

Xi³

et al. 2022

Front. Agron.

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Pairs polyphylla var. yunnanensis is a perennial herb, and its rhizomes are used as medicine to invigorate blood circulation, dissipate blood stasis, relieve swelling and pain, clear heat and detoxify the body. However, due to its slow growth, most of the saponin content is low, while the content of saponins is higher in Paris fargesii Franch of the same genus. The isolation and screening of strains from P. fargesii Franch that promote the accumulation of saponin content in P. polyphylla var. yunnanensis is important to improve the growth rate of artificially cultivated P. polyphylla var. yunnanensis and increase its medicinal activity. Plant symbiotic strains are biologically active microorganisms that can promote the growth and development of host plants through direct probiotic effects and indirect disease resistance mechanisms. Here, we isolated four strains of symbiotes, including two bacteria, Bacillus cereus (LgD2) and Bacillus thuringiensis (LgD10), and two fungi, Aspergillus tamari (TPD11) and Fusarium oxysporum (TPB). All of them promoted the biomass, chlorophyll content and photosynthetic efficiency of P. polyphylla var. yunnanensis seedlings to different degrees and effectively stimulated the accumulation of saponin content in P. polyphylla var. yunnanensis seedlings to improve the expression of saponin synthesis pathway genes. The symbiotes also promoted the enzymatic activities of the enzymes superoxide dismutase (SOD), peroxidase (POD), polyphenol oxidase (PPO) and phenylalanine aminolase (PAL), improved the physiological indicators of tobacco plants (plant height, crown width, leaf length and leaf width), reduced the incidence of tobacco black shank, and improved the growth and resistance-related genes of tobacco. In conclusion, the four symbiotic strains TPD11, TPB, LgD10 and LgD2 play an important role in regulating the growth, promoting saponin accumulation, and disease resistance of P. polyphylla var. yunnanensis.

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“…Two strains of A. tamari F2 and A. flavus F3 also showed pathogenicity against the bug Elasmolomus pallens with mortality rates of 62% and 90%, respectively after 10 days at a concentration of 1 × 10 8 conidia/mL (Umaru and Simarani 2020). Also, A. flavus, A. fumigutus, and A.niger showed pathogenicity against three grasshoppers O. velox, P. pictus, and H. nigrorepletus, whereas A. tamari showed pathogenicity against mosquitos Aedes aegypti and Culex quinquefasciatus (Baskar et al 2020). This is the first report of pathogenicity of fungi A. tamarii, B. bassiana, and M. anisopliae against the grasshopper O. chinensis.…”

Section: Discussionmentioning

confidence: 95%

“…has been reported as potential entomopathogenic fungi for the control of various grasshoppers and locusts. A. oryzae showed pathogenicity against the locust L. migratoria, whereas A. flavus, A. fumigutus, and A.niger showed pathogenicity against grasshoppers Oxya velox, P. pictus, and Hieroglyphus nigrorepletus (Zhang et al 2015;Baskar et al 2020). However, there has been little research on entomopathogenic fungi against the grasshopper O. chinensis so far.…”

Section: Introductionmentioning

confidence: 99%

Isolation and screening of entomopathogenic fungi against the grasshopper Oxya chinensis (Orthoptera: Acrididae) from rice fields in Thua Thien Hue, Vietnam

Cuong

SOUVANNALATH²,

et al. 2022

Biodiversitas

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Abstract. Cuong LCV, Souvannalath B, Ha TP, Lien LQ, Tam VTT, Hoai PTT, Anh HLT, Dat TTH. 2022. Isolation and screening of entomopathogenic fungi against the grasshopper Oxya chinensis (Orthoptera: Acrididae) from rice fields in Thua Thien Hue, Vietnam. Biodiversitas 23: 4906-4911. The grasshopper Oxya chinensis is one of the most abundant herbivorous insects of rice plants in Vietnam and causes severe damage to the crop. Therefore, biological control has become a particularly valuable method for grasshopper and insect control in agricultural production. The aim of the present study was to isolate and investigate the potential entomopathogenic fungi against the grasshopper O. chinensis from rice fields in Thua Thien Hue, to develop environmentally friendly bio-insecticides. The results showed that 37 fungal isolates were isolated from dead grasshoppers collected from rice fields in Thua Thien Hue province. Results of pathogenicity screening of 12 selected fungal isolates showed that the mortality rate of 4th-instar grasshopper nymphs O. chinensis ranged from 21.99% to 100% after 15 treatment days at a concentration of 1 × 108 conidia/ml. Of these, three fungal isolates, namely NL3, NL4, NL12 showed the highest pathogenicity against the grasshopper O. chinensis with mortality rates of 87.73%, 89.81%, and 100%, respectively after 15 days. LC50 values of NL3, NL4, NL12 isolates against 4th-instar nymphs and adults of O. chinensis ranged from 9.5×104 to 4.8×105 conidia/mL and 7.8 × 106 to 2.5 × 106 conidia/mL, respectively at 7 days. LT50 values of NL3, NL4, NL12 isolates against 4th-instar nymphs and adults of O. chinensis were from 4.1 to 4.9 days and 5.5 to 6.1 days, respectively at a concentration of 1 × 108 conidia/mL. Results from morphological identification and nuclear ribosomal internal transcribed spacer (ITS) gene sequence showed that three potential entomopathogenic fungi were Aspergillus tamarii, Beauveria bassiana, and Metarhizium anisopliae. The pathogenicity of Aspergillus tamarii, Beauveria bassiana, and Metarhizium anisopliae against the grasshopper O. chinensis is reported for the first time. The present investigation revealed that these entomopathogenic fungi could be a useful source for developing environment-friendly bio-insecticides for sustainable agricultural production.

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Larvicidal and histopathology effect of endophytic fungal extracts of Aspergillus tamarii against Aedes aegypti and Culex quinquefasciatus

Cited by 26 publications

References 79 publications

Endophytic Fungi Fusarium Equiseti EF2 Isolated from Leucas Aspera: A Novel Biocontrol Agent Against Culex Sp

Endophytic Fungi Fusarium Equiseti EF2 Isolated from Leucas Aspera: A Novel Biocontrol Agent Against Culex Sp

Isolation and identification of symbiotic strains in Paris fargesii Franch that promote disease resistance

Isolation and screening of entomopathogenic fungi against the grasshopper Oxya chinensis (Orthoptera: Acrididae) from rice fields in Thua Thien Hue, Vietnam

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