Larvicidal and Enzymatic Inhibition Effects of Annona Muricata Seed Extract and Main Constituent Annonacin against Aedes Aegypti and Aedes Albopictus (Diptera: Culicidae)

Rodrigues, Alzeir Machado; Silva, Antonio Adailson Sousa; Pinto, Cleonilda Claita Carneiro; Santos, Dayanne Lima dos; Freitas, José Cláudio Carneiro de; Martins, Víctor Emanuel Pessoa; Morais, Selene Maia de

doi:10.3390/ph12030112

Cited by 19 publications

(11 citation statements)

References 40 publications

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“…However, the larvicidal mechanism action of these compounds was not reported. Annonacin isolated from the seed of A. muricata was evaluated for its larvicidal activity on Aedes aegypti and Aedes albopictus larvae [ 202 ]. The greater larvicidal activity was reported against Aedes aegypti with a LC 50 of 2.65 μg/mL compared to Aedes albopictus with LC 50 of 8.34 μg/mL.…”

Section: Pharmacological Activity Of Isolated Compounds From ...mentioning

confidence: 99%

Traditional Uses, Phytochemistry and Pharmacological Activities of Annonacae

2022

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In 1789, the Annonaceae family was catalogued by de Jussieu. It encompasses tropical and subtropical plants which are widespread in distribution across various continents such as Asia, South and Central America, Australia and Africa. The genus of Annona is one of 120 genera of the Annonaceae family and contains more than 119 species of trees and shrubs. Most species are found in tropical America, where over 105 species have been identified. Due to its edible fruits and medicinal properties, Annona is the most studied genus of Annonaceae family. To date, only a limited number of these species have economic value, including A. squamosa L. (sugar apple), A. cherimola Mill. (Cherimoya), A. muricata L. (guanabana or soursop), A. atemoya Mabb. (atemoya), a hybrid between A. cherimola and A. squamosa, A. reticulata L. (custard apple), A. glabra L. (pond-apple) and A. macroprophyllata Donn. Sm. (ilama). Phytochemically, several classes of secondary metabolites, including acetogenins, essential oils, alkaloids, terpenoids and flavonoids. The pharmacological activities of Annona species leaves and seeds include antibacterial, anticancer, antidiabetic and anti-inflammatory properties.

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Section: Pharmacological Activity Of Isolated Compounds From ...mentioning

confidence: 99%

Traditional Uses, Phytochemistry and Pharmacological Activities of Annonacae

2022

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“…In order to assess whether protein protease inhibitors contribute to the mortality, the extract was digested with Proteinase K. For Annona muricata leaf extract the Proteinase K treatment did not change the protease inhibition significantly and the mortality rate remained the same (Table 5) indicating that that the inhibitor in the extract is not a protein or not degraded by Proteinase K treatment. Rodrigues et al reported the larvicidal effects of A. muricata seed extract against Aedes aegypti and A. albopictus and Annonacin as the major component [35] . But it remains to be seen whether it is the same inhibitor in the leaf showing the larvicidal effect.…”

Section: Toxicity Of Plant Extracts To Fourth Instars Aedes Aegypti Larvae After Proteinase K Treatmentmentioning

confidence: 99%

Toxicity of plant extracts containing trypsin inhibitor to the larvae of Aedes aegypti

Aparna¹,

Parambil²,

George³

et al. 2021

Int. J. Mosq. Res.

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Mosquitoes are vectors of many pathogens and can transmit a range of potentially fatal diseases. Some of these diseases spread via mosquitoes belonging to the genus Aedes include Dengue, Chikungunya, Yellow fever, Zika etc. Conventional methods of mosquito control are not always completely successful. Therefore new strategies need to be adopted for mosquito control. Plant protease inhibitors are promising candidates as they prevent larval growth by inhibiting gut proteases of mosquito larvae. In this study we screened 15 plant extracts containing trypsin inhibitors for their toxicity to the larvae of Aedes aegypti. Leaf extracts from Lawsonia inermis showed the highest mortality with 100% mortality after 48 hours in second, third and in fourth instar larvae. The treatment with extract from Annona muricata leaf led to 100, 96 and 66% mortality after 48 hours in second, third and fourth instar larvae respectively. After Proteinase K treatment of Lawsonia inermis leaf extract, a reduction in the in vitro inhibition of larval gut protease activity from 82 to 6% and a decrease in mortality from 80 to 50% were observed compared to untreated. This revealed that the mortality, at least in part, is due to the presence of a protenaceous plant protease inhibitor in the extract. Expressing the gene coding for such protease inhibitors in micro organisms or algae upon which the mosquito larvae feeds will serve as an alternate method for control of mosquito population.

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“…f. & Thomson reported a potential larvicidal activity (LC 5 0 , 6.95 ppm; LC 9 0 , 30.12 ppm) against C. quinquefasciatus mosquito vector. The acetone seed extract (Rodrigues et al 2019). From the above findings, we can conclude that plant-based products can prove to be the best alternatives or effective source for the control of pest species because these products have minimum LC values and biodegradable nature as mentioned above.…”

Section: Bioassay Studies Of Some Herbal Extracts Against the Larvae mentioning

confidence: 99%

Biocontrol of mosquito vectors through herbal-derived silver nanoparticles: prospects and challenges

Kumar

Singh

et al. 2020

Environ Sci Pollut Res

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Mosquitoes spread several life-threatening diseases such as malaria, filaria, dengue, Japanese encephalitis, West Nile fever, chikungunya, and yellow fever and are associated with millions of deaths every year across the world. However, insecticides of synthetic origin are conventionally used for controlling various vector-borne diseases but they have various associated drawbacks like impact on non-targeted species, negative effects on the environment, and development of resistance in vector species by alteration of the target site. Plant extracts, phytochemicals, and their nanoformulations can serve as ovipositional attractants, insect growth regulators, larvicides, and repellents with least effects on the environment. Such plant-derived products exhibit broad-spectrum resistance against various mosquito species and are relatively cheaper, environmentally safer, biodegradable, easily accessible, and are non-toxic to non-targeted organisms. Therefore, in this review article, the current knowledge of phytochemical sources exhibiting larvicidal activity and their variations in response to solvents used for their extraction is underlined. Also, different methods such as physical, chemical, and biological for silver nanoparticle (AgNPs) synthesis, their mechanism of synthesis using plant extract, their potent larvicidal activity, and the possible mechanism by which these particles kill mosquito larvae are discussed. In addition, constraints related to commercialization of nanoherbal products at government and academic or research level and barriers from laboratory experiments to field trial have also been discussed. This comprehensive information can be gainfully employed for the development of herbal larvicidal formulations and nanopesticides against insecticide-resistant vector species in the near future.

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Larvicidal and Enzymatic Inhibition Effects of Annona Muricata Seed Extract and Main Constituent Annonacin against Aedes Aegypti and Aedes Albopictus (Diptera: Culicidae)

Cited by 19 publications

References 40 publications

Traditional Uses, Phytochemistry and Pharmacological Activities of Annonacae

Traditional Uses, Phytochemistry and Pharmacological Activities of Annonacae

Toxicity of plant extracts containing trypsin inhibitor to the larvae of Aedes aegypti

Biocontrol of mosquito vectors through herbal-derived silver nanoparticles: prospects and challenges

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