Larvicidal activity of Xenorhabdus and Photorhabdus bacteria against Aedes aegypti and Aedes albopictus

Vitta, Apichat; Thimpoo, Punnawat; Meesil, Wipanee; Yimthin, Thatcha; Fukruksa, Chamaiporn; Polseela, Raxsina; Mangkit, Bandid; Tandhavanant, Sarunporn; Thanwisai, Aunchalee

doi:10.4103/2221-1691.221134

Cited by 37 publications

(29 citation statements)

References 6 publications

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“…The general interest on Photorhabdus and Xenorhabdus increased in recent years, not only because of their large number of SMs, but also due to their easy-to-handle cultivation under laboratory conditions in combination with the accessibility for genetic manipulations such as genomic integrations or deletions [14][15][16][17]. Furthermore, recently published studies focused on the possible application of Photorhabdus and Xenorhabdus as biological pest control agents with and without the corresponding nematodes [18,19].…”

Section: Introductionmentioning

confidence: 99%

Fabclavine diversity in Xenorhabdus bacteria

Wenski

Çimen

Berghaus

et al. 2020

Beilstein J. Org. Chem.

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The global threat of multiresistant pathogens has to be answered by the development of novel antibiotics. Established antibiotic applications are often based on so-called secondary or specialized metabolites (SMs), identified in large screening approaches. To continue this successful strategy, new sources for bioactive compounds are required, such as the bacterial genera Xenorhabdus or Photorhabdus. In these strains, fabclavines are widely distributed SMs with a broad-spectrum bioactivity. Fabclavines are hybrid SMs derived from nonribosomal peptide synthetases (NRPS), polyunsaturated fatty acid (PUFA), and polyketide synthases (PKS). Selected Xenorhabdus and Photorhabdus mutant strains were generated applying a chemically inducible promoter in front of the suggested fabclavine (fcl) biosynthesis gene cluster (BGC), followed by the analysis of the occurring fabclavines. Subsequently, known and unknown derivatives were identified and confirmed by MALDI–MS and MALDI–MS2 experiments in combination with an optimized sample preparation. This led to a total number of 22 novel fabclavine derivatives in eight strains, increasing the overall number of fabclavines to 32. Together with the identification of fabclavines as major antibiotics in several entomopathogenic strains, our work lays the foundation for the rapid fabclavine identification and dereplication as the basis for future work of this widespread and bioactive SM class.

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Section: Introductionmentioning

confidence: 99%

Fabclavine diversity in Xenorhabdus bacteria

Wenski

Çimen

Berghaus

et al. 2020

Beilstein J. Org. Chem.

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“…aegypti and Ae. albopictus larvae were susceptible to symbiotic bacteria, with high mortality after exposure to P. luminescens , X. nematophila (da Silva et al, ), X. stockiae (bNBP22.2_TH), X. indica (bKK26.2_TH) (Vitta et al, ), X. ehlersii bMH9.2_TH, X. stockiae (bLPA18.4_TH) (Fukruksa et al, ), X. stockiae (bNN112.3_TH), and P. luminescens subsp. akhurstii (bNN121.4_TH) (Yooyangket et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Cell suspensions of Xenorhabdus and Photorhabdus have also been reported as biocontrol agents against Aedes spp. in the laboratory (Da Silva et al, ; Fukruksa et al, ; Vitta et al, ; Yooyangket et al, ). Therefore, Xenorhabdus and Photorhabdus are interesting alternative larvicides for controlling Aedes mosquitoes.…”

Section: Introductionmentioning

confidence: 99%

Survey of entomopathogenic nematodes and associate bacteria in Thailand and their potential to control Aedes aegypti

Suwannaroj

Yimthin

Fukruksa

et al. 2020

J Applied Entomology

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Aedes aegypti is an insect vector that transmits several viruses affecting humans worldwide. Entomopathogenic nematodes (EPNs) and their symbiotic bacteria are organisms with the potential to control many insects. In this study, we did a survey aimed to identify EPNs and their symbiotic bacteria and evaluate the larvicidal activity of bacteria against Ae. aegypti. We collected 540 soil samples from 108 sites in Phitsanulok Province, lower northern Thailand. Baiting techniques and White traps were used to isolate EPNs from soil samples. By sequencing of 28S rDNA and internal transcribed spacer regions, 51 EPN isolates were identified as Steinernema surkhetense (35 isolates), Heterorhabditis indica (14 isolates) and Heterorhabditis sp. SGmg3 (two isolates). Based on sequencing of a partial region of the recA gene, 35 isolates of Xenorhabdus were identified as Xenorhabdus stockiae, and 20 Photorhabdus isolates were identified as Photorhabdus luminescens subsp. akhurstii (10 isolates), P. luminescens subsp. hainanensis (seven isolates) and P. asymbiotica subsp. australis (three isolates). Screening for larvicidal activity of bacteria against Ae. aegypti was performed in the laboratory. Xenorhabdus WB5.4 and Xenorhabdus WB12.5, which were closely related to X. stockiae, resulted in high mortality of Ae. aegypti (99.99% and 70%, respectively) at 96 hr after exposure. Comparing with control groups, mortality of Ae. aegypti larvae was low (1.11%–6.67%) after exposure for 24–96 hr. Our findings showed the potential of X. stockiae for controlling Ae. aegypti. Further studies are needed to elucidate the mechanisms through which these bacteria kill Ae. aegypti larvae.

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“…Thus, new experiments are needed to determine whether the microbial isolates are producers of secondary metabolites with tick-control action or whether the microbial cells themselves produce an effect against these arachnids, and then define what is the best strategy for using these microorganisms as candidate agents for biological control against tick species.In this work, it was demonstrated that there are sustainable alternatives for the biological control of vectors that are responsible for diseases of great epidemiological impact and that affect thousands of people in Brazil, such as Dengue, Zika, Chikungunya and Yellow Fever.The larvicidal action of microbial suspensions on larvae of the mosquito Aedes aegypti was observed by the reduction of movement speed of the vectors when they come in contact with the suspensions, which possibly contain compounds of toxic action for the vectors. This toxicity can be caused mainly by the oral route in immature stages of the larvae, a mechanism also reported in 201812 , which was termed as oral toxicity by ingestion, in this case performed by the ingestion of microbial cells by larvae of the mosquito Aedes aegypti and not by contact of a chemical compound. Another possible action would be the interference with normal chemical reactions of the nervous system, disabling the normal movement of the vector, representing a mechanism of action similar to compounds of the classes of organophosphates, carbamates and pyrethroids.…”

mentioning

confidence: 85%

“…The efficacy of bacteria of the species Xenorhabdus indica, X. stockiae and Photorhabdus hainanensis in the control of Aedes aegypti mosquitoes was proven through the death of larvae due to exposure to bacteria. 12 In the same context, researchers evaluated the effect of the fungus Metarhizium anisopliae on larvae of the mosquito Aedes aegypti in its second larval stage (L2), with mortality ranging from 10 to 100% . 13 In the biological control used against ticks, strains of entomopathogenic fungi can be evaluated for their ability to interrupt the life cycle of various insects and arachnids.…”

Section: Introductionmentioning

confidence: 99%

Uso de microrganismos de efluente industrial no controle biológico de vetores

Favato

Cecchet

Cardeal

et al. 2020

Rev Epidemiol Control Infect

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Justificativa e Objetivos: a utilização de microrganismos como controle biológico de vetores sanitários pode ser considerada uma prática menos agressiva ao ambiente, em comparação com os produtos químicos utilizados. O presente estudo avaliou a eficiência de suspensões celulares de fungos e bactérias isolados de efluentes industriais têxteis no controle sanitário dos vetores naturais Aedes aegypti e Dermacentor nitens como alternativa sustentável de controle biológico. Métodos: foram avaliadas sete linhagens de fungos e seis de bactérias. Os isolados foram cultivados em caldo nutriente e caldo de batata, para bactérias e fungos, respectivamente. Alíquotas de 2 mL de cada suspensão microbiana foram adicionadas diretamente nas larvas dos mosquitos e nos carrapatos adultos. Foram analisadas alterações de movimentação e paralisação dos vetores em diferentes tempos de exposição entre zero e 20 minutos e três e 24 horas. Resultados: duas bactérias e um fungo promoveram uma desaceleração dos movimentos e/ou um aumento da movimentação dos ectoparasitas logo após a administração. Dois isolados bacterianos promoveram a paralisação dos movimentos de uma larva do mosquito Aedes aegypti em seu primeiro estágio de desenvolvimento, enquanto que um fungo provocou aumento da movimentação das larvas em seu estágio de desenvolvimento mais avançado. Conclusão: os microrganismos mostraram potencial uso no controle de vetores sanitários. Testes subsequentes de atividade dos possíveis metabólitos secundários produzidos e das formas de administração das culturas microbianas serão executados. Os resultados encontrados encorajam futuros estudos de otimização e caracterização dos extratos celulares, os quais poderão ser utilizados como ferramenta sustentável no controle biológico.

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Larvicidal activity of Xenorhabdus and Photorhabdus bacteria against Aedes aegypti and Aedes albopictus

Cited by 37 publications

References 6 publications

Fabclavine diversity in Xenorhabdus bacteria

Fabclavine diversity in Xenorhabdus bacteria

Survey of entomopathogenic nematodes and associate bacteria in Thailand and their potential to control Aedes aegypti

Uso de microrganismos de efluente industrial no controle biológico de vetores

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