Larvicidal activity of pectolinaringenin from Clerodendrum phlomidis L. against Culex quinquefasciatus Say and Aedes aegypti L. (Diptera: Culicidae)

Abstract

Larvicidal activity of 12 fractions and a compound of chloroform extract of Clerodendrum phlomidis L. (Lamiaceae) was assayed for their toxicity against the early fourth-instar larvae of the filarial vector Culex quinquefasciatus Say and dengue vector Aedes aegypti L. The fractions were tested at 100-, 50-, 25- and 12.5-ppm concentrations. The compound pectolinaringenin was tested at 5-, 2.5-, 1.0- and 0.5-ppm concentrations. Among the different fractions, fraction 5 recorded the lowest LC(50) and LC(90) values of 5.02, 61.63 ppm and 32.86, 73.62 ppm against C. quinquefasciatus and A. aegypti, respectively. The compound pectolinaringenin showed the lowest LC(50) and LC(90) values of 0.62, 2.87 ppm and 0.79, 5.31 ppm against C. quinquefasciatus and A. aegypti, respectively. This is the first report on the mosquito larvicidal activity of the isolated compound pectolinaringenin from C. phlomidis. The results of this study show that the chloroform extract of C. phlomidis can be used as a potent source and pectolinaringenin as a new natural mosquito larvicidal agent.

Larvicidal and Pupicidal Activities of Alizarin Isolated from Roots of Rubia cordifolia Against Culex quinquefasciatus Say and Aedes aegypti (L.) (Diptera: Culicidae)

“…In the present study, fraction 2 from the methanol extract of roots of R. cordifolia eluted with hexane:ethyl acetate (90:10) recorded good mosquitocidal activity against A. aegypti followed by fraction 19. LC 50 and LC 90 values for fraction 2 for third instars of C. quinquefasciatus and A. aegypti corroborated earlier findings of Muthu et al (2012) of the potential of plant extracts-Clerodendrum phlomidis-as sources of active molecules with insecticide activity against C. quinquefasciatus and A. aegypti.…”

Evaluation of fractions and 5,7-dihydroxy-4',6-dimethoxy-flavone fromClerodendrum phlomidis Linn. F. against Helicoverpa armigera Hub.

“…Extraction, fractionation and isolation of pectolinaringenin Extraction, fractionation and isolation of pectolinaringenin from C. phlomidis were similar as reported by Muthu et al (2012a).…”

“…Fraction 5 at 1000 ppm and pectolinaringenin at 100 ppm exhibited potent larvicidal activity with least LC 50 values of 198.23 and 27.31ppm, respectively against H. armigera in this investigation. Similarly, Muthu et al (2012a) reported that fraction 5 exhibited LC 50 5.02 and 61.63 ppm and pectolinaringenin LC 50 0.62 and 2.87 ppm against C. quinquefasciatus and A. aegypti, respectively. At lower concentration (12.5 ppm), the compound exhibited more than 38% larval mortality against H. armigera.…”

“…EI-MS was taken on SHIMADZU instrument at 70ev by the direct inlet method. All the chemicals and solvents used were of analytical grade (Muthu et al 2012a). …”

Bioefficacy of flindersine against Helicoverpa armigera Hübner, Spodoptera litura Fabricius, Anopheles stephensis Liston. and Culex quinquefasciatus Say.

“…quinquefasciatus and A. aegypti (Muthu et al 2012). Similarly, essential oil and their compounds sabinene, biofloratriene, borneol and β-Bisabolol from Clausena dentate (Rutaceae) exhibited larvicidal activity against A. aegypti (Rajkumar and Jebanesan 2010).…”