Larvicidal activity of fatty acid methyl esters of Vitex species against Culex quinquefasciatus

Kannathasan, K.; Senthilkumar, A.; Venkatesalu, V.; Chandrasekaran, M.

doi:10.1007/s00436-008-1078-1

Cited by 66 publications

(33 citation statements)

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“…A survey of literature on control of different species of mosquito revealed that assessment of the efficacy of different phytochemicals obtained from various plants has been carried out by a number of researches on the field of vector control. A number of plant extracts have been reported to have mosquitocidal or repellent activities against mosquito vectors, but very few plant products have shown practical utility for mosquito control (Sukumar et al 1991;Shaalan et al 2005;Kalyanasundaram and Das 1985;Kalyanasundaram and Babu 1982;Kannathasan et al 2007Kannathasan et al , 2008Pandey et al 2007;Rahuman et al 2008a, b). The plant products can be obtained either from the whole plant or from a specific part by extraction with different types of solvents such as aqueous, methanol, chloroform, and hexane, depending on the polarity of the phytochemicals.…”

Section: Discussionmentioning

confidence: 96%

“…Phytochemicals derived from plant sources can act as larvicides, insect growth regulators, repellents, and oviposition attractants and can play an important role in the interruption of the transmission of mosquito-borne diseases at the individual as well as at the community level (Babu and Murugan 1998;Venketachalam and Jebasan 2001a, b;Mittal and Subbarao 2003;Rahuman et al 2008a;Senthilkumar et al 2008;Kannathasan et al 2008;Ghosh et al 2008;Bagavan et al 2008). Natural products of plant origin with insecticidal properties have been tried in the recent past for control of variety of insect pests and vectors.…”

Section: Introductionmentioning

confidence: 98%

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Larvicidal activity of Saraca indica, Nyctanthes arbor-tristis, and Clitoria ternatea extracts against three mosquito vector species

et al. 2008

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Screening of natural products for mosquito larvicidal activity against three major mosquito vectors Aedes aegypti, Culex quinquefasciatus, and Anopheles stephensi resulted in the identification of three potential plant extracts viz., Saraca indica/asoca, Nyctanthes arbor-tristis, and Clitoria ternatea for mosquito larval control. In the case of S. indica/asoca, the petroleum ether extract of the leaves and the chloroform extract of the bark were effective against the larvae of C. quinquefasciatus with respective LC(50) values 228.9 and 291.5 ppm. The LC(50) values of chloroform extract of N. arbor-tristis leaves were 303.2, 518.2, and 420.2 ppm against A. aegypti, A. stephensi, and C. quinquefasciatus, respectively. The methanol and chloroform extracts of flowers of N. arbor-tristis showed larvicidal activity against larvae of A. stephensi with the respective LC(50) values of 244.4 and 747.7 ppm. Among the methanol extracts of C. ternatea leaves, roots, flowers, and seeds, the seed extract was effective against the larvae of all the three species with LC(50) values 65.2, 154.5, and 54.4 ppm, respectively, for A. stephensi, A. aegypti, and C. quinquefasciatus. Among the three plant species studied for mosquito larvicidal activity, C. ternatea was showing the most promising mosquito larvicidal activity. The phytochemical analysis of the promising methanolic extract of the seed extract was positive for carbohydrates, saponins, terpenoids, tannins, and proteins. In conclusion, bioassay-guided fractionation of effective extracts may result in identification of a useful molecule for the control of mosquito vectors.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 98%

Larvicidal activity of Saraca indica, Nyctanthes arbor-tristis, and Clitoria ternatea extracts against three mosquito vector species

et al. 2008

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“…Changbunjong et al (11) showed that ethanolic extract of unripe S. xanthocarpum fruit had larvicidal activity against C. quinquefasciatus, with LC 50 and LC 90 values of 573.20 and 1,066.93mg/L, respectively, indicating that the extract was less toxic than that tested in this study. Kannathasan et al (12) evaluated the larvicidal activity of fatty acid methyl esters (FAMEs) obtained from 3 species of Vitex against C. quinquefasciatus larvae and reported LC 50 values ranging from 9.25 to 18.64mg/L. In this study, MEUF and MERF showed the lowest LC 50 values (3.72 and 2.16mg/L, respectively) of the test treatments, indicating more potent larvicidal activity than FAMEs isolated from Vitex species.…”

Section: Fruit Of S Lycocarpummentioning

confidence: 66%

Larvicidal activity of oils, fatty acids, and methyl esters from ripe and unripe fruit of Solanum lycocarpum (Solanaceae) against the vector Culex quinquefasciatus (Diptera: Culicidae)

Silva

Neto

Alves

et al. 2015

Rev. Soc. Bras. Med. Trop.

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Introduction:The larvicidal activity of oils, fatty acids, and methyl esters of Solanum lycocarpum fruit against Culex quinquefasciatus is unknown. Methods: The larvicidal activity of samples of ripe and unripe fruit from S. lycocarpum was evaluated against third and fourth instar larvae of C. quinquefasciatus. Results: The oils, fatty acids, and methyl esters of S. lycocarpum showed the greatest larvicidal effect (57.1-95.0%) at a concentration of 100mg/L (LC 50 values between 0.70 and 27.54mg/L). Conclusions: Solanum lycocarpum fruit may be a good source of new natural products with larvicidal activity.Keywords: Bioassay. Mosquito. Pesticide. Synthetic insecticides are used to control mosquito vectors of diseases in several parts of the world. However, resistance to synthetic insecticides has recently become problematic in vector control programs. Thus is important the development of new products, with the capacity to prevent or minimize the resistance, to combat insects (1) . Biological products represent alternative approaches for preventing the development of resistance in mosquitoes.Plants contain bioactive compounds with insecticidal properties that could be suitable for mosquito control applications (2) . Several species of the genus Solanum have demonstrated larvicidal and pupicidal activities against the mosquito Culex quinquefasciatus (3) (4) (5) . Solanum lycocarpum A. St. Hil. (Solanaceae) is popularly known as the fruit of the wolf and is widely distributed in the Brazilian Cerrado (6) . Solanum lycocarpum is commonly used in traditional medicine as a sedative and a treatment for epilepsy, asthma, diabetes, obesity, abdominal pain, renal pain, and high cholesterol levels (7) . Despite the widespread medicinal usage of S. lycocarpum and the reported insecticidal properties of other Solanum species, no studies have been conducted on the insecticidal activity of oils, fatty acids, and methyl esters obtained from the ripe and unripe fruit of S. lycocarpum.Fruit of S. lycocarpum A. St. Hil. were collected in São Sebastião do Oeste, Minas Gerais, Brazil in August 2011. The plant material was identifi ed by Dr. Alexandre Salino. A voucher specimen (BHCB 159397) was deposited at the Instituto de Ciências Biológicas Herbarium, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. Samples of the dried and powdered unripe (170.01g) and ripe (250.58g) fruit were subjected to oil extraction using a Soxhlet extractor with petroleum ether as the solvent (Vetec®, São Paulo, Brazil; 700mL, 6h). The extracted oils were concentrated in a rotary evaporator at 50°C under reduced pressure to produce 26.95g of oil of unripe fruit (OUF) and 29.09g of oil of ripe fruit (ORF).Fatty acids and methyl esters were isolated from S. lycocarpum via transesterifi cation (8) . OUF and ORF (2g each) were refl uxed with 1.0 mol/L methanolic NaOH solution for 30 min and extracted with ethyl ether. The aqueous phases were acidifi ed with 1.0 mol/L HCl solution and extracted with ethyl ether to obtain fatty...

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“…Studies with fatty acid methyl esters are few, especially regarding their larvicidal activity against C. quinquefasciatus larvae. However, it was observed that FAMEs obtained from three species of Vitex showed larvicidal activity against C. quinquefasciatus (Kannathasan et al 2008). …”

Section: Introductionmentioning

confidence: 97%

The Influence of Fatty Acid Methyl Esters (FAMEs) in the Biochemistry and the Na+/K+-ATPase Activity of Culex quinquefasciatus Larvae

et al. 2016

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Culex quinquefasciatus is the main vector of lymphatic filariasis and combating this insect is of great importance to public health. There are reports of insects that are resistant to the products currently used to control this vector, and therefore, the search for new products has increased. In the present study, we have evaluated the effects of fatty acid methyl esters (FAMEs) that showed larvicidal activity against C. quinquefasciatus, on glucose, total protein, and triacylglycerol contents and Na(+)/K(+)-ATPase activity in mosquito larvae. The exposure of the fourth instar larvae to the compounds caused a decrease in the total protein content and an increase in the activity of the Na(+)/K(+)-ATPase. Furthermore, the direct effect of FAMEs on cell membrane was assessed on purified pig kidney Na(+)/K(+)-ATPase membranes, erythrocyte ghost membranes, and larvae membrane preparation. No modifications on total phospholipids and cholesterol content were found after FAMEs 20 min treatment on larvae membrane preparation, but only 360 µg/mL FAME 2 was able to decrease total phospholipid of erythrocyte ghost membrane. Moreover, only 60 and 360 µg/mL FAME 3 caused an activation of purified Na(+)/K(+)-ATPase, that was an opposite effect of FAMEs treatment in larvae membrane preparation, and caused an inhibition of the pump activity. These data together suggest that maybe FAMEs can modulate the Na(+)/K(+)-ATPase on intact larvae for such mechanisms and not for a direct effect, one time that the direct effect of FAMEs in membrane preparation decreased the activity of Na(+)/K(+)-ATPase. The biochemical changes caused by the compounds were significant and may negatively influence the development and survival of C. quinquefasciatus larvae.

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Larvicidal activity of fatty acid methyl esters of Vitex species against Culex quinquefasciatus

Cited by 66 publications

References 9 publications

Larvicidal activity of Saraca indica, Nyctanthes arbor-tristis, and Clitoria ternatea extracts against three mosquito vector species

Larvicidal activity of Saraca indica, Nyctanthes arbor-tristis, and Clitoria ternatea extracts against three mosquito vector species

Larvicidal activity of oils, fatty acids, and methyl esters from ripe and unripe fruit of Solanum lycocarpum (Solanaceae) against the vector Culex quinquefasciatus (Diptera: Culicidae)

The Influence of Fatty Acid Methyl Esters (FAMEs) in the Biochemistry and the Na+/K+-ATPase Activity of Culex quinquefasciatus Larvae

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