Larval stop, delayed development and survival in overcrowded cultures of Drosophila melanogaster: Effect of urea and uric acid

Botella, Luisa María; Moya, Andrés; González, M. C.; Ménsua, J. L.

doi:10.1016/0022-1910(85)90118-0

Cited by 81 publications

(86 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In studies of interspecific competition between Drosophila species, the inhibitory effects of larval biotic residues on both larval viability (Budnick & Brncic, 1974, 1976 and female fecundity (Aiken & Gibo, 1979) were observed. Subsequently, exposure to urea was shown to result in the slower development and reduced survivorship of D. melanogaster larvae (Botella et a!., 1983(Botella et a!., , 1985. In our laboratory, we have also observed an inhibition of fecundity in female D. melanogaster maintained as adults on…”

Section: Introductionsupporting

confidence: 64%

“…Although it is not the primary metabolic waste of Drosophila larvae, urea has been shown to accumulate in Drosophila culture vials during the larval phase (Botella et a!., 1985). In our laboratory, we have observed that the rate at which nitrogenous waste accumulates in the food medium is much higher in crowded cultures, although it is not clear at this time whether the principal form of nitrogenous waste building up in these cultures is urea or ammonia, because the technique used converts urea to ammonia in order to assay urea level (A. G. Mueller, unpublished data).…”

Section: Introductionmentioning

confidence: 99%

“…Initially, such studies focused on understanding the biology of traits either known, or thought, to be advantageous under conditions of extreme larval crowding. These traits ranged from primarily behavioural attributes such as pupation height (Sameoto & Miller, 1968;Markow, 1979), larval feeding rate (Burnet et a!., 1977) and the extent of larval locomotory activity while feeding (Sokolowski, 1985;Godoy-Herrera, 1986) to those that were predominantly physiological, such as larval efficiency at converting food to biomass (de Miranda & Eggleston, 1988) and larval tolerance to nitrogenous metabolic waste (Botella et a!., 1985). Subsequently, the relevance of many of these traits to the density-*Correspondence.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genetics of larval urea tolerance in Drosophila melanogaster

1996

View full text Add to dashboard Cite

The genetic control of larval tolerance to urea, a nitrogenous waste-product occurring naturally in crowded Drosophila cultures, was investigated in a set of five laboratory populations of D. melanogaster that had been successfully subjected to selection for increased larval urea tolerance. Larva to adult survivorship and development time at three different levels of urea were assayed on the five selected populations, their five matched controls and a set of 10 F1 hybrid populations derived from reciprocal crosses between pairs of selected and control populations. As expected from the results of previous studies, the selected populations exhibited greater larval tolerance to the toxic effects of urea, relative to their controls. Comparison of the hybrid and parental populations with respect to both survivorship and development time indicated that the genetic control of urea tolerance in the selected populations is largely dominant, and has a significant X-linked component. The data also suggested that females from the selected populations exercise a nongenetic maternal effect on the development time of their progeny, regardless of urea level.

show abstract

Section: Introductionsupporting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetics of larval urea tolerance in Drosophila melanogaster

1996

View full text Add to dashboard Cite

show abstract

“…One of the parameters which may account for the superiority or inferiority of strains is their higher or lower resistance to larval biotic residues which pollute the competition media. Although the presence of other biotic residues cannot be ruled out, attention was focused on uric acid because of its noxious effect on larvae, and because high levels accumulate in crowded cultures (B OTELLA et al, 1985). Urea effects were also studied.…”

Section: Resultsmentioning

confidence: 99%

Intra- and intergenotypic larval competition in Drosophila melanogaster : effect of larval density and biotic residues

CASTRO¹,

BOTELLA²,

MENSUA³

1987

Genet. Sel. Evol.

View full text Add to dashboard Cite

SummaryOptimal density (maximum adult production) was determined in 8 strains of Drosophila melanogaster reared on small amounts of food. The amount of uric acid excreted by each strain into the medium was also determined, as well as the resistance to toxic products (urea and uric acid) added to the media.Competitive dicultures between these strains were later established so that the total seeding density corresponded to optimal densities of each competitor. In these competitive systems, regressions higher than first order were found. The outcome for viability and developmental time in dicultures is explained in terms of the parameters studied in the monocultures : viability, developmental time, intrinsic mortality, optimal density, excretion of uric acid, and resistance to biotic wastes.

show abstract

“…Most previous studies on this topic have shown that HD conditions slow down the development rate (Lints & Lints, 1971;Boetella et a!., 1985), the theoretical assumption being that slower development somehow led to the long life span. However, Yonemura et a!.…”

Section: Larval Development and Adult Longevitymentioning

confidence: 99%

Larval regulation of adult longevity in a genetically-selected long-lived strain of Drosophila

et al. 1993

View full text Add to dashboard Cite

Our previous work has shown that the major genes involved in the expression of the extendedlongevity phenotype are located on the third chromosome. Furthermore, their expression is negatively and positively influenced by chromosomes 2 and 1, respectively. In this report we show that the expression of the extended-longevity phenotype is dependent on the larval environment. A controlled chromosome substitution experiment was carried out using a strain selected for long life (L) and its parent (R) strain. Twenty different combinations of the three major chromosomes were conducted and their longevities were determined under both high (HD) and low (LD) larval density conditions. The extended-longevity phenotype was only expressed under RD conditions. The chromosome interactions were not apparent under LD conditions. Density-shift experiments delineate a critical period for expression of the extended-longevity phenotype, extending from 60 h after egg laying (AEL) to 96 h AEL, during which the developing animal must be exposed to HD conditions if the extended-longevity phenotype is to be expressed. The change from HD to LD conditions is accompanied by statistically significant increases in body weight. The possible role of a dietary restriction phenomenon is examined and the implications of these findings discussed. It is now apparent, however, that the extended-longevity phenotype in Drosophila is a developmental genetic process.

show abstract

Larval stop, delayed development and survival in overcrowded cultures of Drosophila melanogaster: Effect of urea and uric acid

Cited by 81 publications

References 16 publications

Genetics of larval urea tolerance in Drosophila melanogaster

Genetics of larval urea tolerance in Drosophila melanogaster

Intra- and intergenotypic larval competition in Drosophila melanogaster : effect of larval density and biotic residues

Larval regulation of adult longevity in a genetically-selected long-lived strain of Drosophila

Contact Info

Product

Resources

About