Larval oral exposure to thiacloprid: Dose-response toxicity testing in solitary bees, Osmia spp. (Hymenoptera: Megachilidae)

Claus, Gregor; Pisman, Matti; Spanoghe, Pieter; Smagghe, Guy; Eeraerts, Maxime

doi:10.1016/j.ecoenv.2021.112143

Cited by 17 publications

(13 citation statements)

References 35 publications

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“…Here we found changes in gene expression in response to two classes of neonicotinoids with thiacloprid exposure affecting the expression of more genes, including immune genes, than imidacloprid. This is in line with other studies that have looked at thiacloprid exposed red mason bees and observed impairment in immunity (Brandt et al, 2020) or larval development (Claus et al, 2021). For both neonicotinoids, we find significantly more genes up-regulated in response to pesticide exposure than expected, suggesting that overall the exposure to pesticides results in an active response of heightened gene expression as opposed to a mere passive shift in gene expression.…”

Section: Discussionsupporting

confidence: 92%

Genomic architecture and sexually dimorphic expression underlying immunity in the red mason bee, Osmia bicornis

Moellmann

Colgan

2021

Preprint

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Insect pollinators provide crucial ecosystem services yet face increasing environmental pressures. The challenges posed by novel and reemerging pathogens on bee health means we need to improve our understanding of the immune system, an important barrier to infections and disease. Despite its importance, for certain ecologically important species, such as solitary bees, our understanding of the genomic basis and molecular mechanisms underlying immune potential, and how intrinsic and extrinsic factors may influence immune gene expression is lacking. Here, to improve our understanding of the genomic architecture underlying immunity of a key solitary bee pollinator, we characterised putative immune genes of the red mason bee, Osmia bicornis. In addition, we used publicly available RNA-seq datasets to determine how sexes differ in immune gene expression and splicing but also how pesticide exposure may affect immune gene expression in females. Through comparative genomics, we reveal an evolutionary conserved set of more than 500 putative immune-related genes. We found genome-wide patterns of sex-biased gene expression, including immune genes involved in antiviral-defence. Interestingly, the expression of certain immune genes were also affected by exposure to common neonicotinoids, particularly genes related to haemocyte proliferation. Collectively, our study provides important insights into the gene repertoire, regulation and expression differences in the sexes of O. bicornis, as well as providing additional support for how neonicotinoids can affect immune gene expression, which may affect the capacity of solitary bees to respond to pathogenic threats.

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Section: Discussionsupporting

confidence: 92%

Genomic architecture and sexually dimorphic expression underlying immunity in the red mason bee, Osmia bicornis

Moellmann

Colgan

2021

Preprint

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show abstract

“…Here we found changes in gene expression in response to two classes of neonicotinoids with thiacloprid exposure affecting the expression of more genes, including immune genes, than imidacloprid. This is in line with other studies that have examined thiacloprid exposed red mason bees and observed impairment in immunity (Brandt et al, 2020) or larval development (Claus et al, 2021). For both neonicotinoids, we find significantly more genes up‐regulated in response to pesticide exposure than expected, suggesting that overall the exposure to pesticides results in an active response of heightened gene expression as opposed to a mere passive shift in gene expression.…”

Section: Discussionsupporting

confidence: 92%

Genomic architecture and sexually dimorphic expression underlying immunity in the red mason bee, Osmia bicornis

Möllmann

Colgan

2022

Insect Molecular Biology

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Insect pollinators provide crucial ecosystem services yet face increasing environmental pressures. The challenges posed by novel and reemerging pathogens on bee health means we need to improve our understanding of the immune system, an important barrier to infections and disease. Despite the importance of solitary bees, which are ecologically relevant, our understanding of the genomic basis and molecular mechanisms underlying their immune potential, and how intrinsic and extrinsic factors may influence it is limited. To improve our understanding of the genomic architecture underlying immunity of a key solitary bee pollinator, we characterized putative immune genes of the red mason bee, Osmia bicornis. In addition, we used publicly available RNA‐seq datasets to determine how sexes differ in immune gene expression and splicing but also how pesticide exposure may affect immune gene expression in females. Through comparative genomics, we reveal an evolutionarily conserved set of more than 500 putative immune‐related genes. We found genome‐wide patterns of sex‐biased gene expression, with greater enrichment of immune‐related processes among genes with higher constitutive expression in males than females. Our results also suggest an up‐regulation of immune‐related genes in response to exposure to two common neonicotinoids, thiacloprid and imidacloprid. Collectively, our study provides important insights into the gene repertoire, regulation and expression differences in the sexes of O. bicornis, as well as providing additional support for how neonicotinoids can affect immune gene expression, which may affect the capacity of solitary bees to respond to pathogenic threats.

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“… 2 Draft version available for Osmia species (Roessink et al, 2018). 3 Proposal for a test protocol available for two Osmia species (Claus et al., 2021 ). …”

Section: Effect Assessment In Lower Tiersmentioning

confidence: 99%

Revised guidance on the risk assessment of plant protection products on bees (Apis mellifera, Bombus spp. and solitary bees)

Adriaanse¹,

Arce²,

Focks³

et al. 2023

EFS2

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The European Commission asked EFSA to revise the risk assessment for honey bees, bumble bees and solitary bees. This guidance document describes how to perform risk assessment for bees from plant protection products, in accordance with Regulation (EU) 1107/2009. It is a review of EFSA's existing guidance document, which was published in 2013. The guidance document outlines a tiered approach for exposure estimation in different scenarios and tiers. It includes hazard characterisation and provides risk assessment methodology covering dietary and contact exposure. The document also provides recommendations for higher tier studies, risk from metabolites and plant protection products as mixture.

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Larval oral exposure to thiacloprid: Dose-response toxicity testing in solitary bees, Osmia spp. (Hymenoptera: Megachilidae)

Cited by 17 publications

References 35 publications

Genomic architecture and sexually dimorphic expression underlying immunity in the red mason bee, Osmia bicornis

Genomic architecture and sexually dimorphic expression underlying immunity in the red mason bee, Osmia bicornis

Genomic architecture and sexually dimorphic expression underlying immunity in the red mason bee, Osmia bicornis

Revised guidance on the risk assessment of plant protection products on bees (Apis mellifera, Bombus spp. and solitary bees)

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