Larval migration of the zoonotic parasite Anisakis pegreffii (Nematoda: Anisakidae) in European anchovy, Engraulis encrasicolus: Implications to seafood safety

Cipriani, Paolo; Acerra, Virginia; Bellisario, Bruno; Sbaraglia, Gian Luca; Cheleschi, Roberto; Nascetti, Giuseppe; Mattiucci, Simonetta

doi:10.1016/j.foodcont.2015.04.043

Cited by 98 publications

(84 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…from fish viscera to the muscle if the fish are stored whole. Cipriani et al 19. reported no significant increase in the A. pegreffii infection rates in anchovy muscle when fish were refrigerated at 2 °C when examined 24, 48 and 72 h after capture in the Mediterranean Sea.…”

Section: Discussionmentioning

confidence: 97%

“…Anisakis simplex s.l. (probably belonging to A. simplex s.s. and A. pegreffii , since mixed infection of both Anisakis species are commonly found in fish species inhabiting the Iberian Atlantic coast5) and A. pegreffii have been reported in European anchovy from the Gulf of Cádiz and Strait of Gibraltar (Iberian Atlantic coast)18, and from Mediterranean Sea1920, respectively, and their prevalence and intensity of infection in this fish may vary depending on the fishing area and season181920.…”

mentioning

confidence: 98%

See 1 more Smart Citation

Assessing the risk of an emerging zoonosis of worldwide concern: anisakiasis

Bao

Pierce

Pascual

et al. 2017

Sci Rep

Self Cite

141

110

View full text Add to dashboard Cite

Anisakiasis is an emerging zoonosis caused by the fish parasitic nematode Anisakis. Spain appears to have the highest reported incidence in Europe and marinated anchovies are recognised as the main food vehicle. Using data on fishery landings, fish infection rates and consumption habits of the Spanish population from questionnaires, we developed a quantitative risk assessment (QRA) model for the anchovy value chain. Spaniards were estimated to consume on average 0.66 Anisakis per untreated (non-frozen) raw or marinated anchovy meal. A dose-response relationship was generated and the probability of anisakiasis was calculated to be 9.56 × 10−5 per meal, and the number of annual anisakiasis cases requiring medical attention was predicted between 7,700 and 8,320. Monte Carlo simulations estimated post-mortem migration of Anisakis from viscera to flesh increases the disease burden by >1000% whilst an education campaign to freeze anchovy before consumption may reduce cases by 80%. However, most of the questionnaire respondents who ate untreated meals knew how to prevent Anisakis infection. The QRA suggests that previously reported figures of 500 anisakiasis per year in Europe is a considerable underestimate. The QRA tool can be used by policy makers and informs industry, health professionals and consumers about this underdiagnosed zoonosis.

show abstract

Section: Discussionmentioning

confidence: 97%

mentioning

confidence: 98%

Assessing the risk of an emerging zoonosis of worldwide concern: anisakiasis

Bao

Pierce

Pascual

et al. 2017

Sci Rep

Self Cite

141

110

View full text Add to dashboard Cite

show abstract

“…Certain geographic locations of fish stocks also develop reputations for being parasite‐rich due to the relative abundance of intermediate or definitive hosts and can be avoided by fishing vessels . After being caught, fish may be gutted immediately to prevent larvae migrating from the viscera into the flesh after the death of the fish and/or the increase in temperature . Some authors were unable to detect migration of Anisakis larvae to the muscles of fish post‐mortem, while others did find increased larvae in the muscles over time .…”

Section: Introductionmentioning

confidence: 99%

Anisakis – immunology of a foodborne parasitosis

Nieuwenhuizen

2016

Parasite Immunology

View full text Add to dashboard Cite

SummaryAnisakis species are marine nematodes which can cause zoonotic infection in humans if consumed in raw, pickled or undercooked fish and seafood. Infection with Anisakis is associated with abdominal pain, nausea and diarrhoea and can lead to massive infiltration of eosinophils and formation of granulomas in the gastrointestinal tract if the larvae are not removed. Re-infection leads to systemic allergic reactions such as urticarial or anaphylaxis in some individuals, making Anisakis an important source of hidden allergens in seafood. This review summarizes the immunopathology associated with Anisakis infection. Anisakiasis and gastroallergic reactions can be prevented by consuming only fish that has been frozen to −20°C to the core for at least 24 hours before preparation. Sensitization to

show abstract

“…The different relative abundance of these L3 among these microhabitats in fish can be affected by many factors such as the parasites species, the fish species, the fish age, and the environmental conditions (Lymbery and Cheah 2007). Although the mechanisms of larval penetration within fish hosts are still unknown, some studies revealed that L3 migrate from the visceral organs to the muscle after the death of the fish hosts and this postmortem migration is thought to be also affected by interactions among parasites, host, and external environments (Cipriani et al 2016;Lymbery and Cheah 2007). Thus, the risk of human infection can be enhanced if the fish are ingested whole or if the fish have been kept without evisceration for a period of time.…”

Section: Discussionmentioning

confidence: 99%

Genetic identification of anisakid nematodes isolated from largehead hairtail (Trichiurus japonicus) in Korea

2016

View full text Add to dashboard Cite

Background: The nematode species belonging to genus Anisakis occur at their third larval stage in numerous marine teleost fish species worldwide and known to cause accidental human infection through the ingestion of raw or undercooked fish or squids. They may also draw the attention of consumers because of the visual impact of both alive and dead worms. Therefore, the information on their geographical distribution and clear species identification is important for epidemiological survey and further prevention of human infection. Results: For identification of anisakid nematodes species isolated from largehead hairtail (Trichiurus japonicus), polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of internal transcribed spacers of ribosomal DNA were conducted. Mitochondrial cytochrome c oxidase subunit 2 gene was also sequenced, and phylogenetic analysis was conducted. From the largehead hairtail (n = 9), 1259 nematodes were isolated in total. Most of the nematodes were found encapsulated throughout the viscera (56.2 %, 708/1259) or moving freely in the body cavity (41.5 %, 523/1259), and only 0.3 % (4/1259) was found in the muscles. By PCR-RFLP, three different nematode species were identified. Anisakis pegreffii was the most dominantly found (98. 7 %, 1243/1259) from the largehead hairtail, occupying 98.7 % (699/708) of the nematodes in the mesenteries and 98.1 % (513/523) in the body cavity. Hybrid genotype (Anisakis simplex × A. pegreffii) occupied 0.5 %, and Hysterothylacium sp. occupied 0.2 % of the nematodes isolated in this study. Conclusions:The largehead hairtail may not significantly contribute accidental human infection of anisakid nematode third stage larvae because most of the nematodes were found from the viscera or body cavity, which are not consumed raw. But, a high prevalence of anisakid nematode larvae in the largehead hairtail is still in concern because they may raise food safety problems to consumers. Immediate evisceration or freezing of fish after catch will be necessary before consumption.

show abstract

Larval migration of the zoonotic parasite Anisakis pegreffii (Nematoda: Anisakidae) in European anchovy, Engraulis encrasicolus: Implications to seafood safety

Cited by 98 publications

References 40 publications

Assessing the risk of an emerging zoonosis of worldwide concern: anisakiasis

Assessing the risk of an emerging zoonosis of worldwide concern: anisakiasis

Anisakis – immunology of a foodborne parasitosis

Genetic identification of anisakid nematodes isolated from largehead hairtail (Trichiurus japonicus) in Korea

Contact Info

Product

Resources

About